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We narrowed to 599 results for: arl

Showing: 181 - 200 of 599 results

  1. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...that other technologies need to be discarded but clearly we are in an era of neuroscience research where...where optogenetics will be a standard tool used in nearly any neuroscience project or topic of interest. ...elsewhere (here and here for instance) [2], [3]. Karl Deisseroth of Stanford University , one of the key...

  2. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...brain along with high off-target expression, particularly in the liver, when administered intravenously...different efficacy and tropism between NHP species, particularly between New World and Old World monkeys. Selecting...but its neuron transduction encompasses more molecularly-defined neuronal subtypes and with less bias ... to AAV9 in the pigtail macaque brain slices. Similarly, AAV9-X1.1 outperformed the other capsids in the...

  3. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...search for "CRISPR", short-hand for Clustered Regularly Interspaced Short Palindromic Repeats, in Pubmed...the PAM sequence is immediately adjacent to the nearly-homologous target sites. The mere presence of additional...pluripotent stem cells differing exclusively at two early onset Parkinson Point mutations. Cell, 146(2), 318...

  4. Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More

    Type
    Blog Post
    ...Listen to Our Segment on mCyRFP1 Hot Plasmid 4: mScarlet - a new, bright, and monmeric red fluorescent ...fluorescent protein.  Listen to Our Segment on mScarlet Special thanks to our sound engineer/producer, Eddy Page...

  5. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    ...been an important animal model for research, particularly genetics research, for decades. Its short life...during our stay there. Yet capturing the flies is nearly impossible, due to their small size and inconspicuous...

  6. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...brain tissue is injected with compounds that intracellularly spread away from the site of application and...transport has been observed for AAV1, -5, -8 (McFarland et al., 2009), and bidirectional transport for...Neuroscience 193, 249–258. PubMed PMID: 21782903. McFarland, N.R., Lee, J.S., Hyman, B.T., and McLean, P.J...

  7. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    ...envelope vector pMD2.G This plasmid has been requested nearly 6,000 times (over 800 orders in 2017) and expresses...variety of cells and study their effects. To date, nearly 900 articles cite the use of pMD2.G obtained from...

  8. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    ...electricity! A (not so) shocking transition  In early July the Town of Watertown announced the creation... electricity will allow us to maintain these particularly energy-intensive systems in a more environmentally...

  9. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...Jordane Dimidschstein New serotype pAAV_hSyn-PdCO-mScarlet-WPRE AAV1 Optogenetics Ofer Yizhar New viral ...pAAV-Ef1a-fDIO hChR2(H134R)-EYFP AAVrg Optogenetics Karl Deisseroth New viral tool AiP13987: pAAV-AiE0452h...

  10. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...service with multiple serotypes pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE AAV1, AAV5 Optogenetics Yizhar New...with multiple serotypes pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE AAV1, AAV5 Optogenetics Yizhar New viral ...

  11. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    ...., 2024), developed and deposited at Addgene by Arlene Sharpe’s lab, enables in vivo screens in naïve ...here! Marty LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and...

  12. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    ...Newland. Newland is General Counsel at Atalanta, an early-stage biotech company that is developing RNAi therapeutics...up going straight to law school. This was in the early 80s, and biotech was really just taking off. I found...

  13. Newly Updated AAV Data Hub!

    Type
    Blog Post
    ...more. A great submission includes: Images that clearly show viral spread and cellular tropism* (min. 300...serotype An abstract that summarizes your experiment clearly and concisely* *required for a DOI. Reports that...

  14. Science Communication for Everyone

    Type
    Blog Post
    ... of Toronto. The Addictive Brain was founded in early 2018 with the goal of communicating science to non-scientists... eLife ambassador, SfN community leader, and an early research career panelist at BioTechniques.     Additional...

  15. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ...Scientists by Jennifer Tsang These tips were written early on in the pandemic. Later in the year, Joanne Kamens...methods for extracting DNA without a kit too. 1. Early Career Researcher Toolbox: Free Online Molecular...

  16. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    ...mouse, fruit fly, yeast, zebrafish, and worm. 4. Early Career Researcher Toolbox: Free Tools for Making...from the community. Are there any topics you particularly like or maybe something you’d like to see more...

  18. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    ... systems, developed and deposited at Addgene by Arlene Sharpe’s lab, allow for wide deployment of in vivo...here!   Marty LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and...
Showing: 181 - 200 of 599 results