We narrowed to 310 results for: bon

1. Cloning Mammalian Cells with the Agarose Method

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   Blog Post

   ...diluted from 10X and containing both Hepes and bicarbonate) is brought to room temperature and placed in...

2. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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   ...what do you want to make?   References: Jerome Bonnet et al. "Amplifying genetic logic gates." Science...

3. Immunology Research Plasmids and Resources

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   ...BMP5 bone morphogenetic protein 5 MGC34244 BMP6 bone morphogenetic protein 6 VGR, VGR1 BMP7 bone morphogenetic... MGC34632 BMP1 bone morphogenetic protein 1 FLJ44432, PCOLC, PCP, TLD, pCP-2 BMP10 bone morphogenetic ...protein 10 MGC126783 BMP15 bone morphogenetic protein 15 GDF9B, ODG2, POF4 BMP2 bone morphogenetic protein ...protein 2 BMP2A BMP3 bone morphogenetic protein 3 BMP-3A BMP4 bone morphogenetic protein 4 BMP2B, BMP2B1, MCOPS6...protein 7 OP-1 BMP8A bone morphogenetic protein 8a FLJ14351, FLJ45264 BMP8B bone morphogenetic protein...BMPR1A bone morphogenetic protein receptor, type IA 10q23del, ACVRLK3, ALK3, CD292, SKR5 BMPR1B bone morphogenetic...

4. Antibodies 101: Producing Recombinant Antibodies

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   ...periplasmic space to be oxidized and form disulfide bonds (Frenzel et. al., 2013). If you’re using a mammalian...

5. DNA Purification Without a Kit

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   ...extraction protocol) disrupts the DNA’s hydrogen bonds with water molecules and coaxes it into sticking...

6. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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   .... S. cerevisiae) Plasmid Type: Choose an empty backbone if you intend to clone your own insert, or use...

7. Simplify Cloning with in vivo Assembly

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   ...immediate co-transformation with linearized vector backbone. DpnI digestion - 1 μl DpnI is added to the...

8. Plasmids 101: Secondary Nanobody Toolbox

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   ...Maleimide-labeling occurs when a maleimide group forms a covalent bond with a sulfhydryl group (–SH), such as that found...

9. Isolating Genomic Regions of Interest with the CRISPR System

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   ...enCh IP technology using recombinant CRISPR ribonucleoproteins." Genes to Cells 21.4 (2016): 370-377. PubMed...

10. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    Blog Post

    ...is irreversible — FP fragments become covalently bonded by associating with one another — which means it...

11. Cpf1: A New Tool for CRISPR Genome Editing

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    ...eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins (RNPs) failed to induce off-target editing...

12. Plasmids 101: Protein tags

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    ...such as Thioredoxin A (TrxA) that improve disulfide bond formation in order to help keep your protein soluble...

13. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    ...addition, we have recently developed the oligoribonucleotide (ORN) interference-PCR (ORNi-PCR) method ...

14. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    ...Click here to view Addgene’s collection of empty backbones for constructing fluorescent fusions. Transcription...

15. CRISPR Plasmids - Empty gRNA Vectors

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    ...S. pyogenes de Bono sgRNA with rpr-1 promoter 48961 Worm EcoRI none S. pyogenes de Bono pMB60 47941 Worm... cut S. pyogenes Puro Zhang sgRNA(MS2) cloning backbone 61424 Mammalian BbsI none S. pyogenes Zhang pSpCas9n...none S. pyogenes Puro Zhang lenti sgRNA(MS2)_zeo backbone 61427 Mammalian/Lentiviral BsmBI none S. pyogenes...BsmBI none S. pyogenes Joung AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR 68347 Mammalian... cut S. pyogenes mCherry Kuhn AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR 60229 Mammalian/AAV SapI... SapI none S. pyogenes Zhang AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR 60231 Mammalian...AAV SapI none S. pyogenes Zhang AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR 60226 Mammalian...

16. Plasmids 101: Shuttle Vectors

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    ...Resources on Addgene.org Collection of empty plasmid backbones ...

17. Plasmids 101: Stringent Regulation of Replication

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    ...BioRender.com. Once DnaA breaks the local hydrogen bonds holding the DNA together, it recruits helicases ...

18. Viral Production at Addgene

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    ...responsive element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for...

19. Viral Vectors 101: Preparing Pooled Libraries

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    ...with a protocol for a library of similar size and backbone.  Verification Once your library has been amplified...

20. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    ...making it a more flexible cutter with the added bonus of low off-target effects. The SpG variant, discovered...