We narrowed to 312 results for: bon
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TypeCollection...BMP5 bone morphogenetic protein 5 MGC34244 BMP6 bone morphogenetic protein 6 VGR, VGR1 BMP7 bone morphogenetic... MGC34632 BMP1 bone morphogenetic protein 1 FLJ44432, PCOLC, PCP, TLD, pCP-2 BMP10 bone morphogenetic ...protein 10 MGC126783 BMP15 bone morphogenetic protein 15 GDF9B, ODG2, POF4 BMP2 bone morphogenetic protein ...protein 2 BMP2A BMP3 bone morphogenetic protein 3 BMP-3A BMP4 bone morphogenetic protein 4 BMP2B, BMP2B1, MCOPS6...protein 7 OP-1 BMP8A bone morphogenetic protein 8a FLJ14351, FLJ45264 BMP8B bone morphogenetic protein...BMPR1A bone morphogenetic protein receptor, type IA 10q23del, ACVRLK3, ALK3, CD292, SKR5 BMPR1B bone morphogenetic...
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Cloning Mammalian Cells with the Agarose Method
TypeBlog Post...diluted from 10X and containing both Hepes and bicarbonate) is brought to room temperature and placed in... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog Post...Protein Timers Browse All Fluorescent Protein Empty Backbones Check Out Our Fluorescent Protein Guide ... -
Plasmids 101: Protein Expression
TypeBlog Post... include ribosomes, RNA polymerase, tRNAs, ribonucleotides and amino acids. Cell-free expression systems... -
Polymerase Chain Reaction Overview and Applications
TypeBlog Post...the dsDNA template to a point where the hydrogen bonds break between the base pairs. This results in the... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog Post...what do you want to make? References: Jerome Bonnet et al. "Amplifying genetic logic gates." Science... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...S. pyogenes de Bono sgRNA with rpr-1 promoter 48961 Worm EcoRI none S. pyogenes de Bono pMB60 47941 Worm... cut S. pyogenes Puro Zhang sgRNA(MS2) cloning backbone 61424 Mammalian BbsI none S. pyogenes Zhang pSpCas9n...none S. pyogenes Puro Zhang lenti sgRNA(MS2)_zeo backbone 61427 Mammalian/Lentiviral BsmBI none S. pyogenes...BsmBI none S. pyogenes Joung AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR 68347 Mammalian... cut S. pyogenes mCherry Kuhn AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR 60229 Mammalian/AAV SapI... SapI none S. pyogenes Zhang AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR 60231 Mammalian...AAV SapI none S. pyogenes Zhang AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR 60226 Mammalian... -
Simplify Cloning with in vivo Assembly
TypeBlog Post...immediate co-transformation with linearized vector backbone. DpnI digestion - 1 μl DpnI is added to the... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog Post...Maleimide-labeling occurs when a maleimide group forms a covalent bond with a sulfhydryl group (–SH), such as that found... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog Post...enCh IP technology using recombinant CRISPR ribonucleoproteins." Genes to Cells 21.4 (2016): 370-377. PubMed... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...is irreversible — FP fragments become covalently bonded by associating with one another — which means it... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog Post...periplasmic space to be oxidized and form disulfide bonds (Frenzel et. al., 2013). If you’re using a mammalian... -
DNA Purification Without a Kit
TypeBlog Post...extraction protocol) disrupts the DNA’s hydrogen bonds with water molecules and coaxes it into sticking... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog Post.... S. cerevisiae) Plasmid Type: Choose an empty backbone if you intend to clone your own insert, or use... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog Post...eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins (RNPs) failed to induce off-target editing... -
Plasmids 101: Protein tags
TypeBlog Post...such as Thioredoxin A (TrxA) that improve disulfide bond formation in order to help keep your protein soluble... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog Post...addition, we have recently developed the oligoribonucleotide (ORN) interference-PCR (ORNi-PCR) method ... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog Post...Click here to view Addgene’s collection of empty backbones for constructing fluorescent fusions. Transcription... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post...interesting behaviors. There is diversity in the social bonds that they form including genetic mechanisms that... -
Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog Post...O, Jinek M (2016) Structural basis for the endoribonuclease activity of the type III-A CRISPR-associated...