We narrowed to 314 results for: bon

... make your own Fragmid components! Ensure your backbone and genes contain only the Bbsl cut sites needed...

...100–115 mM = 0.7%–0.8% (v/v) Breaks disulfide bonds HCl Adjust pH to 6.7–6.8 Adjust pH ...

...at the Faculty of Sciences of NOVA University in Lisbon, Portugal. She leads the Glycoimmunology group ...

...only occur when galactose replaces glucose as the carbon source. Student results have shown that most, but...

...gRNA expression can be achieved using lentiviral backbones expressing blasticidin or puromycin resistance...

...primary cells. Although cell entry of a Cas9 ribonucleoprotein (RNP) was limited (likely due to its long,...

.../10.1038/s41598-019-41088-2 Magnus C, Lee P, Bonaventura J, Zemla R, Gomez J, Ramirez M, Hu X, Galvan ...

...reader-effector proteins. b) Golden Gate assembly (backbones available at Addgene) with examples of potential...

...eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins (RNPs) failed to induce off-target editing...

...Schnütgen F, Doerflinger N, Calléja C, Wendling O, Chambon P, and Ghyselinck NB. A directional strategy for...

...before the event... Choose a moderator with a backbone. It is nice to have a subject area expert, but...

...diluted from 10X and containing both Hepes and bicarbonate) is brought to room temperature and placed in...

...Protein Timers Browse All Fluorescent Protein Empty Backbones Check Out Our Fluorescent Protein Guide ...

...Check Out Our CRISPR Guide Pages Find Empty gRNA Backbones for Your Experiments Get Ready-to-Use CRISPR Lentivirus...

... include ribosomes, RNA polymerase, tRNAs, ribonucleotides and amino acids. Cell-free expression systems...

...the dsDNA template to a point where the hydrogen bonds break between the base pairs. This results in the...

...what do you want to make?   References: Jerome Bonnet et al. "Amplifying genetic logic gates." Science...

...is irreversible — FP fragments become covalently bonded by associating with one another — which means it...

...extraction protocol) disrupts the DNA’s hydrogen bonds with water molecules and coaxes it into sticking...

...periplasmic space to be oxidized and form disulfide bonds (Frenzel et. al., 2013). If you’re using a mammalian...