We narrowed to 10 results for: bon

...pAdEasy-1 Adenoviral backbone plasmid that lacks E1 and E3. pAdEasy-2 Adenoviral backbone plasmid that lacks...transgene of interest and both ITRs. Adenoviral backbone plasmid (e.g. pAdEasy) — containing the adenoviral...containing pAdEasy. pAdEasy is a ~33 kb adenoviral backbone plasmid containing the adenoviral genes necessary...production. The shuttle plasmid and the adenoviral backbone plasmid have matching left and right homology ...recombination of the transgene into the adenoviral backbone plasmid. One can also co-transform standard BJ5183...transgene has been inserted into the adenoviral backbone plasmid, and that other patterns of recombination...equivalent across multiple viruses. The adenoviral backbone plasmid pAdEasy-1 (Addgene #16400) is suitable...

...nucleotides on the opposing strands of DNA form hydrogen bonds, where A always pairs with T, and C always pairs...as from a chromosome to a plasmid or vector DNA backbone. These tools are essential to the field of recombinant...of a DNA fragment with a plasmid or vector DNA backbone generates a recombinant DNA molecule, which can...unique and not located elsewhere in the plasmid backbone. In expression plasmids, the MCS is often located...entry vectors and TOPO cloning vectors. Empty Backbones , Cloning Kits Expression Plasmids Used for gene...promoter is used to initiate transcription. Empty Backbones Gene Knock-down Plasmids Used for reducing the...a helicase, the enzyme that breaks the hydrogen bonds between nucleotides on opposing strands. A DNA polymerase...

...been created in AAV backbones for in vivo experiments and in a retroviral backbone for delivery to cells... gRNA and Cas9 protein, which will form a ribonucleoprotein complex through interactions between the gRNA...disrupt Cas9’s interactions with DNA phosphate backbone HypaCas9 — increase Cas9 proofreading and discrimination...endonuclease. Nature , 513 (7519), 569–573. PMID: 25079318 Bondy-Denomy, J., Pawluk, A., Maxwell, K. L., & Davidson...system. Nature , 493 (7432), 429–432. PMID: 23242138 Bondy-Denomy, J., Garcia, B., Strum, S., Du, M., Rollins... Maddalo, D., Manchado, E., Concepcion, C. P., Bonetti, C., Vidigal, J. A., Han, Y., Ogrodowski, P., Crippa...Marino, N. D., Pinilla-Redondo, R., Csörgő, B., & Bondy-Denomy, J. (2020). Anti-CRISPR protein applications...

...compatible restriction enzymes that cut your insert or backbone at only the desired location for your cloning ... your gene of interest (insert) into a plasmid backbone. Created with BioRender.com. Gateway Recombination...disadvantage of TOPO cloning is that very few plasmid backbones are available TOPO-ready, and it is not feasible...overhangs can be efficiently ligated into any plasmid backbone. A drawback of this system is that Gibson assembly...Cloning (MoClo) Guide Choosing Your Perfect Plasmid Backbone Genome Eengineering Guide Addgene Protocols Plasmid...

...terminators, 4 nonfunctional "dummy" parts, 34 backbones, and 6 destination vectors for use in E. coli ...including CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for genome editing in monocots ...expression Christopher Dupont 95 plasmids including backbones, fluorescent proteins, protein tags, and signal...

...10.1038/npp.2012.251 PMID: 23303063 Gomez, J. L., Bonaventura, J., Lesniak, W., Mathews, W. B., Sysa-Shah, ..., Harvey, B. K., Dannals, R. F., Pomper, M. G., Bonci, A., & Michaelides, M. (2017). Chemogenetics revealed...science.1206606 PMID: 21885782 Magnus CJ, Lee PH, Bonaventura J, Zemla R, Gomez JL, Ramirez MH, Hu X, Galvan...

...the Cas enzyme and gRNA, or as a functional ribonucleoprotein (RNP). Created with BioRender.com. When choosing...delivery Lastly, you can deliver Cas-gRNA ribonucleoprotein (RNP) complexes directly into your target ...

... Plasmids within a given library have the same backbone, but they express or target different genes. Some...

... Plasmids within a given library have the same backbone, but they express or target different genes. Some...

...there are other envelope gene options, including: Gibbon Ape Leukemia Virus (GALV) — T and B cell transduction...