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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 376 results for: bre

Showing: 181 - 200 of 376 results

  2. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...Nihongaki, et al., 2015) resulting in a double strand break (DSB) that can be repaired by either non-homologous...References Bubeck F, Hoffmann MD, Harteveld Z, Aschenbrenner S, Bietz A, Waldhauer MC, Börner K, Fakhiri ...

  3. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...targeted known residues on the outer surface to break dimerization interfaces (affecting many of the RFPs...of cytosine to thymine without a double stranded break. However, the method was initially limited by off-target...

  4. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...Single Strand Annealing (SSA) in the event of a DNA break. Next, the gRNA being tested is expressed along ... simplifying experiments when additional cross-breeding steps are undesired or infeasible. Finally, an...

  5. CRISPR Guide

    Type
    Guide
    ...discovered. Browse Plasmids: Single-Strand Break (Nick) , Double-Strand Break (Cut) Increasing Cas9 PAM flexibility...the target DNA. This results in a double-strand break (DSB) within the target DNA, located ∼3–4 nucleotides...small precision edits. NHEJ directly ligates the break ends without the need for a homologous template.... experimentally. Browse Plasmids: Double-Strand Break (Cut) Multiplex Genome Engineering Many CRISPR experiments...Cas3 Rather than initiating DNA cleavage to form a break, Cas3 initiates single-strand DNA degradation. This...running into other proteins bound to DNA, and the break can be repaired by the cell’s endogenous repair ...conversion in RNA or DNA without a DNA double-strand break Cas C RISPR As sociated Protein, includes nucleases...
Showing: 181 - 200 of 376 results