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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 7 results for: bre

Showing: 1 - 7 of 7 results

  1. CRISPR Guide

    Type
    Guide
    ...discovered. Browse Plasmids: Single-Strand Break (Nick) , Double-Strand Break (Cut) Increasing Cas9 PAM flexibility...the target DNA. This results in a double-strand break (DSB) within the target DNA, located ∼3–4 nucleotides...small precision edits. NHEJ directly ligates the break ends without the need for a homologous template.... experimentally. Browse Plasmids: Double-Strand Break (Cut) Multiplex Genome Engineering Many CRISPR experiments...Cas3 Rather than initiating DNA cleavage to form a break, Cas3 initiates single-strand DNA degradation. This...running into other proteins bound to DNA, and the break can be repaired by the cell’s endogenous repair ...conversion in RNA or DNA without a DNA double-strand break Cas C RISPR As sociated Protein, includes nucleases...

  2. Molecular Biology Reference

    Type
    Guide
    ...bases, adenine, thymine, cytosine, and guanine (abbreviated to A, T, C, and G respectively) that are organized...process of molecular cloning enabled scientists to break chromosomes down to study their genes, marking the...series covers topics ranging from the newest breakthroughs in plasmid technologies and research, to overviews...

  3. Promoters

    Type
    Guide
    ...series , covers topics ranging from the newest breakthroughs in plasmid technologies and research to overviews...

  4. Cloning

    Type
    Guide
    ...protocol for restriction cloning and an in-depth breakdown of restriction digests on our website. Restriction...

  5. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...v3060677 PMID: 21994751 Mekkaoui, L., Tejerizo, J. G., Abreu, S., Rubat, L., Nikoniuk, A., Macmorland, W., Horlock...

  6. Adenovirus Guide

    Type
    Guide
    ... Chimpanzee adenoviral vectors as vaccines for outbreak pathogens . Human Vaccines & Immunotherapeutics...

  7. Antibody Guide

    Type
    Guide
    ...UV light. Shear the samples using sonication to break DNA up into fragments of 300-1000 bps in length....
Showing: 1 - 7 of 7 results