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We narrowed to 227 results for: cas9 expression plasmid

Showing: 181 - 200 of 227 results

  1. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ... Check out All the Plasmids from the Collin's Lab Find Synthetic Biology Plasmids for Your Next Experiment...amplification, new toehold switch designs, CRISPR/Cas9-mediated genotyping, as well as our electronic reader...Components." Cell (2016). PubMed PMID: 27160350. Find Plasmids from the publication at Addgene 2. Green, Alexander...switches: de-novo-designed regulators of gene expression." Cell 159.4 (2014): 925-939. PubMed PMID: 25417166...Experiment Deposit Your Plasmids with Addgene ...

  2. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...tables below for CRISPR-FokI plasmids. Plasmids are available for expression in mammalian systems and Drosophila...Drosophila. Mammalian ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter...Yeast ID Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids that should...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR... CRISPR: dCas9-FokI Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...Tools CRISPR Blog Posts A catalytically inactive Cas9 (dCas9) is fused to FokI nuclease. When FokI dimerizes... be added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve...

  3. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...CRISPR/Cas9 Tips for a first time AAV user Resources on Addgene.org Find Adenovirus Plasmids Adenovirus...for model organisms.    Q11: How consistent is expression is when injecting CAP-B10 or CAP-B22 in marmosets...

  4. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...epitopes. Detection of overexpressed (tagged or untagged) protein When expression plasmids are available, or...of interest when expressed at endogenous levels (Laflamme et al., 2019). CRISPR-Cas9 approaches are commonly...flow cytometry, and mRNA expression.   If your protein of interest is expressed at similar levels in ... involves the transient transfection of expression plasmids in a given cell line. For flow cytometry, ...useful to produce a sample with mixed expression, with the expression measurable through an epitope tag such...differences between endogenous expression and transfected overexpression, this approach cannot confirm ...caused by overexpression. Additionally, if the cell line used has endogenous levels of expression, this can...

  5. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...nuclease-deficient Cas9 can bind loci in the genome that are defined by a gRNA. Since Cas9 is a protein, a... "Live cell imaging of phosphoinositides with expressed inositide binding protein domains." Methods 46.3...protein fusions Browse the Fluorescent Proteins Plasmid Kits ...

  6. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...activity. To drive selective A → T editing, they co-expressed Polη, a translesion synthesis polymerase known...inserted the deaminase into the C-terminal domain of Cas9, which narrowed the editing window and reduced A...site. Like in most other base editor designs, the Cas9 nicks the non-edited strand to encourage the cell...reporters Resources on Addgene.org Browse CRISPR plasmids for base editing Get a broad overview with our.... gGBE consists only of engineered MPG fused to nCas9. First, MPG removes the G base from DNA to create...

  7. Lentivirus Production

    Type
    Protocol
    ...DNAs: Lentiviral Packaging plasmid(s), Envelope plasmid, and Transfer plasmid encoding your insert of interest... cm dish). Using transfer plasmid pHAGE TRE dCas9-KRAB (total μg of plasmid DNA 27.8 μg), this would be...transfection plasmids into OptiPro SFM: Reagent Amount per 10 cm dish* Packaging Plasmid ( psPAX2 ) 1.3...1.3 pmol Envelope Plasmid ( pMD2.G ) 0.72 pmol Transfer Plasmid* 1.64 pmol OptiPro SFM to total volume...volume 500 μL *Plasmid concentrations and ratios should be optimized for each transfer plasmid. Pro-Tip Endotoxins...therefore, plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, ... 293T cells were transfected with the GFP-expression plasmid pRosetta using μg total DNA to μg PEI ratios...

  8. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...Unpublished or pre-publication plasmids": Unpublished plasmids or plasmids that will not be published are...and entering a plasmid name,plasmid type, and plasmid description/experimental purpose (right). On the...with your plasmids, and adding all the plasmids at once increases the likelihood that the plasmids are given...easy for you to share plasmids. To achieve this goal, we will archive any plasmids you've deposited with...Spreadsheet". Start your plasmid deposit There are two options to begin your plasmid deposit. Figure 2: Choose... Choose to submit plasmid(s) online or by using a spreadsheet. The plasmid information can be entered ...than 10 plasmids but we recommend using the spreadsheet option for deposits of 10 or more plasmids.   ...

  9. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...below for CRISPR Cascade-Cas3 plasmids. Plasmids are available for expression in mammalian systems, bacteria...the Cascade complex to express equally. To combat this, some CRISPR-Cas3 plasmids take an all-in-one approach...plants. Mammalian ID Plasmid Gene/Insert Promoter PI Publication Bacteria ID Plasmid Gene/Insert Promoter...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions. CRISPR... CRISPR Cas3 CRISPR Plasmids: Cascade-Cas3...Cascade-Cas3 Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...

  10. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...for mammalian RNAi Browse all plasmids for RNAi Check out Addgene's plasmids and resources for plant biology...transgene. Typically, the desired target of RNAi is expressed as inverted repeats separated by a loop region... easiest approach to induce RNAi involves the expression of long hairpin RNA (hpRNA). This method is facilitated...construct has unique features, such as inducible expression of the hpRNA and/or fusing your gene target sequence...harvested instead of microscopic isolation. The plasmids required to conduct an APT-based RNAi experiment... target sequence, as well as essential control plasmids. Many thanks to our guest blogger Robert Orr...Bassik MC (2016) Systematic comparison of CRISPR/Cas9 and RNAi screens for essential genes. Nat Biotechnol...

  11. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...popular split-FP plasmids from our catalog to help you find the right split-FP plasmid for your experiment... vitro, and can be used for measuring protein expression or localization in cells, or even single-particle...below, and click the Article link to find all the plasmids from the associated paper:   Color Base FP...and experimental design. We hope you find these plasmids useful. Good luck, and happy experimenting! References...Split-horseradish peroxidase (Alice Ting Lab 2016)  Split-Cas9 for inducible genome editing (Feng Zhang Lab 2015...

  12. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ... in a plasmid with loxP sites such that their expression is turned on or off in cells expressing Cre. ...allows the visualization of fluorescent proteins expressed in the specimen.  2. Fluorescence Activated Cell...immune lineage, scientists could easily track GFP expressing cells. For example, by harvesting lymph nodes...efforts have been made to create transgenic mice expressing fluorescent protein reporter constructs in the...above depicts the infiltration of neutrophils expressing LysM-GFP (green) into a draining lymph node after...other technologies such as optogenetics and CRISPR/Cas9 will help immunologists create even better tools...Check out Our Fluorescent Protein Guide Pages Find Plasmids for Subcellular Localization Find Fluorescent ...

  13. CRISPR Plasmids - gRNAs

    Type
    Collection
    ... any of these gRNA plasmids and review the publication associated with each plasmid for more information...gRNA Plasmids Please note that the following table lists both published and unpublished plasmids containing...information on the experimental use of these plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...species or PAM binding variant of Cas9. For instance, wild-type SpCas9 must be used with targets that are...gRNA expression vectors available from Addgene. CRISPR... CRISPR gRNAs CRISPR Plasmids: gRNAs Browse CRISPR Plasmids...

  14. Hot Plasmids - August 2020

    Type
    Blog Post
    ...Addgene #140702) The pMAK461-64 plasmids were expressed in SHuffle T7 Express competent E.coli (NEB) using...all-in-one dCas9-KRAB and dCas9-VPR targeting plasmids can be found at Addgene. Check out these plasmids for ...approaches rely on continual expression of both dCas9 and the gRNA. This expression can vary significantly ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...too high expression of these markers can lead to non-specific labeling. They generated plasmids tagged ...easier to clone, express, and purify compared to antibodies. Nanobodies are easily expressed in the cytosol...

  15. CRISPR Guide

    Type
    Guide
    ...Browse Plasmids: Purify Cas9 Alternatives for CRISPR Genome Engineering S. pyogenes Cas9 (SpCas9) is the...more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease... available with multiple Cas9 variants, including high fidelity Cas9s, Cas9s with various PAM requirements...in which Cas9 is included in the gRNA-containing plasmid, or 2-vector systems, in which Cas9 must be delivered...to either co-infect with a Cas9-producing lentivirus or use a Cas9-expressing stable cell line. After applying...strand SpCas9-HF1 - disrupt Cas9’s interactions with DNA phosphate backbone HypaCas9 - increase Cas9 proofreading...new tools. Figure 3: Overview of Cas9n Nickase and dead Cas9 The two Cas9 nuclease domains, RuvC and HNH...

  16. Plan Your Experiment

    Type
    Guide
    .../or Cas9 expression or (2) a lack of efficient target cleavage in cells that do express both Cas9 and ...Figure 1). You will decide how to express Cas9, the delivery system for Cas9 and the guide RNA (gRNA), the...transfect, transduce, or may not express common promoters. Browse Cas9 plasmids for RNP delivery . As mentioned...non-homologous end joining (NHEJ) at the cut site Cas9 or Cas9 nickase High-fidelity Cas enzymes increase specificity...insertions, by copying from a DNA donor template Cas9 or Cas9 fusions with proteins that promote HDR Requires...site. Consider alternative Cas9 enzymes with different PAM sequences or Cas9 enzymes with flexible PAM ...design. Addgene offers many plasmids containing validated gRNAs . These plasmids contain gRNAs that have ...

  17. Modular Cloning Guide

    Type
    Guide
    ...Yeast Expression, Insect Expression Chen Davidovich 41 plasmids to construct multigene expression vectors...methylation-switching. 4G-Cloning Plasmid Kit Bacterial Expression, Insect Expression, Mammalian Expression Stephan Gruber...Other, Yeast Expression, Plant Expression, Bacterial expression Christopher Dupont 95 plasmids including ...You may also like... Plasmid Kits Blog: Plasmids 101 Modular Cloning Blog: Plasmids 101 Modular Cloning...Cloning System Plant Expression Jan Lohmann 56 plasmids to create plant expression vectors containing several...cerevisiae expression. Multiplex Yeast Toolkit (MYT) Yeast Expression, CRISPR Tom Ellis 96 plasmids to extend...Cloning (FTK) Fungal Expression, CRISPR Arnold Driessen 96 plasmids (including CRISPR/Cas9 components) for ...

  18. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...below for CRISPR plasmids to help purify genomic loci. Plasmids are available for expression in mammalian ...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... for enChIP, as well as an anti-Cas9 antibody. Biotin tagging of dCas9 can be achieved by fusing a biotin...biotin acceptor site to dCas9 and co-expressing BirA biotin ligase, as seen in the CAPTURE system. The ...Catalytically inactive CRISPR plasmids for purification of target loci. CRISPR...Purify Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...

  19. Neurodegeneration Research Collection

    Type
    Collection
    ...Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant ORFs... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...linked to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and more....bioRxiv. 2022 Dec 2. Use PiggyBac plasmids with tet-inducible expression of transcription factors for sensory...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ...

  20. Pooled Library Amplifications

    Type
    Blog Post
    ...recombined plasmid to overtake full-size plasmids (or for smaller plasmids to overtake larger plasmids, if the...How many unique plasmids does your library contain?), the variance of size of plasmids in your library...lead to issues such as recombination and loss of plasmid diversity, so Addgene strives to distribute aliquots...library (How important is it that every single plasmid in your library is present, and how will you ensure...30 to 200 times as many colonies as individual plasmids in your library. As an example, if you want 100x... your library, and your library contains 5,000 plasmids, your goal for your transformation would be to...libraries often contain thousands, if not millions, of plasmids, they are generally electroporated into electrocompetent...
Showing: 181 - 200 of 227 results