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We narrowed to 284 results for: cas9 plasmid

Showing: 181 - 200 of 284 results

  1. Plant Plasmids and Resources

    Type
    Collection
    ... Plasmid Collections Plant Plasmids Plant Plasmids and Resources...addition to individual plasmids, Addgene carries several useful toolkits and plasmid collections for use ...for a full selection of plasmids expressing different functional variants of Cas9 and empty gRNA backbones... an intronized Cas9 series of vectors and DNA modules for assembly of CRISPR/Cas9 constructs - Sylvestre...CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants - Niko Geldner. CRISPR-Cas9 vector...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...resources: Find plasmids for use in plants. Plasmid...

  2. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing...transcription none, need Cas9 plasmid Chen Do you have suggestions for other plasmids that should be added...following CRISPR plasmids have been designed for use in Xenopus. Cut Fully functional Cas9 enzymes designed...surrounding the DSB is introduced along with the Cas9 and gRNA plasmids, the cell may instead repair the break ...CRISPR plasmids that have been designed for use in Xenopus. CRISPR... CRISPR Xenopus CRISPR Plasmids: Xenopus Browse... Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation...

  3. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Validated In Resistance Co-expressed Cas9 Depositing...Ampicillin none, need Cas9 plasmid Qi Do you have suggestions for other plasmids that should be added ...gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter...gRNA expression plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert Promoter...population. ID Plasmid Promoter PI Publication Purify A catalytically inactive Cas9 (dCas9) can be used ...Kanamycin none, need Cas9 plasmid Marraffini 42876 pCas9 BsaI E. coli, S. pneumoniae Chloramphenicol yes, cut...efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids By Function Genome...

  4. Editor's Choice, August 2016

    Type
    Blog Post
    ... can use PCR to make small sequence changes to plasmid DNA. This an oldie but a goodie, and in my personal...experience, is a quick way to generate many mutated plasmid constructs. The second post was contributed by ...Viral Transductions Single Base Editing with CRISPR Cas9 Activators: A Practical Guide 10 Steps to a Perfect...uses catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions at...

  5. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...selectable marker, and whether the plasmid contains Cas9. ID Plasmid Expression System Promoter Guide RNA...the tables below for CRISPR prime editing plasmids. Plasmids are available for expression in mammalian...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Drosophila ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...prime editor plasmids for (search and replace) genome editing. CRISPR... CRISPR Prime Edit CRISPR Plasmids: Prime Edit...

  6. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ... tools yet. As a genome editing tool, the CRISPR/Cas9 technology has been surrounded by a whirlwind of...with the post-design process such as primer and plasmid design. Examples:  “multiplex design”, “multi-method... parasite Plasmodium falciparum using the CRISPR-Cas9 system." Nature biotechnology(2014). PubMed PMID... et al. "DNA targeting specificity of RNA-guided Cas9 nucleases." Nature biotechnology 31.9 (2013): 827...Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation." Nature biotechnology...with R. For the Java enthusiasts, take a look at sgRNAcas9. Top tools for transferring CRISPR technology ...

  7. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...the usage of the pSpCas9(BB) plasmid (pX330) (Addgene plasmid ID 42230). This plasmid allows for the simultaneous...utilized, such as pX458 (Addgene plasmid ID 48138) or pX459 (Addgene plasmid ID 48139), which include GFP ...This protocol involves the delivery of CRISPR/Cas9 plasmids by electroporation 4 . This protocol is described.... However, transient transfection of CRISPR/Cas9 plasmids may be successfully adapted to numerous cell... with a PCR cloning kit) into a plasmid vector. Transform the plasmid into DH5α E. coli cells and plate...genomic deletions in mammalian cell lines using CRISPR/Cas9 CRISPR...Mammalian Cell Lines Other Addgene Resources CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud...

  8. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...   It’s not an overstatement to say that CRISPR/Cas9 editing is one of the key tools now providing access...plans and in particular, that they all share the plasmids created under this programs via Addgene. New organisms...sexual reproductive life cycles. One can inject plasmid DNA in the eggs to get transgenic animals. They...-Mediated Ovary Transduction of Cargo)  delivers Cas9 RNP to the arthropod germline by injection into ...

  9. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...without end-point sequencing assays. These plasmids use “Cas9-mediated adenosine transient reporter for... CRISPR plasmids are always being added to the repository. To find all of the CRISPR plasmids available... Xiao Wang Lab has recently deposited a set of plasmids that helps identify base edited cell populations...available from Addgene, head over to our CRISPR Plasmids and Resources page. Here are some highlights from the...Nasse We regularly add new viral aliquots from our plasmid collection to provide ready-to-use viral preps....

  10. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

    Type
    Collection
    ...specifity of Staphylococcus aureus Cas9 (SaCas9 KKH variant) Engineered CRISPR-Cas9 nucleases with altered PAM...the Cas9 endonuclease) that cleaves the target DNA. The Joung lab recently described gRNA and Cas9 expression...CRISPR-Cas/RGN expression plasmids for use in human cells CRISPR-Cas/RGN expression plasmids for use in zebrafish...Guide RNA expression plasmids for the EGFP Reporter Gene Guide RNA expression plasmids for endogenous human...reagents: Cpf1 expression plasmids (AsCpf1 and LbCpf1) for use in mammalian cells SpCas9 variants with no detectable...RNA-Guided Nuclease (RGN) Plasmids Genome... Genome Engineering CRISPR Joung Lab CRISPR Plasmids Joung Lab CRISPR-Cas...

  11. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...SnapGene powered plasmid maps at Addgene  Three factors make our SnapGene powered plasmid maps far easier...easier for you to share plasmid information with colleagues, or explain plasmid features to a lab member... on the main plasmid page under the "Resource Information" section. Analyze your plasmid sequence For ...over from our users: "Please, please improve your plasmid maps!" After thoughtful design, vetting, and tweaking...tweaking, we’re excited to announce that our plasmid and sequence displays are now powered by GSL Biotech's...software and extensive feature library, our updated plasmid and sequence displays are now much easier to interpret...how they’ll make it easier for you to find the plasmids you need. We'll be monitoring and improving the...

  12. Zebrafish Plasmid Collection

    Type
    Collection
    ... Plasmid Collections Zebrafish Plasmids Zebrafish Plasmids and Resources... of the plasmids and tools available at Addgene for use in zebrafish. Browse Plasmids Plasmid Tools for...for plasmids containing your zebrafish gene of interest? Search Addgene's collection for plasmids that...highlights plasmids that contain tools for use in zebrafish research. This includes plasmids for genome...and Cre expression plasmids. Large collections or kits are described below . ID Plasmid Description Gene...CRISPR resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples...Using CRISPR/Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith Joung...

  13. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...need Cas9 plasmid Chen and Wente 42250 DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid Joung...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids that have been designed for use in zebrafish. CRISPR... CRISPR Zebrafish CRISPR Plasmids: Zebrafish...Zebrafish Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional ...Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed for use in zebrafish. Cut Fully...

  14. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...Resources on the Addgene Blog: Plasmids 101: The Promoter Region Plasmids 101: Cre-lox Light-Switchable...Promoter Subtype Promoter Example Activator Addgene Plasmid/Kit Alcohol-regulated AlcA promoter AlcR pGGA008...depositors have developed heat shock-inducible Cre and Cas9 for easy genome engineering in species like C. elegans...use light to regulate transcription. Red flame plasmid pDawn contains the blue-light sensing protein YFI...Systems for E. coli Browse other blog posts from our Plasmids 101 series Resources on Addgene.org: Inducible...Expression Systems at Addgene Cre-lox Guide and Plasmids     ...

  15. Important Considerations When Using AAVs

    Type
    Blog Post
    ...Currently, AAVs are being used to deliver CRISPR/Cas9 but have the potential to deliver other therapeutic...used with new exciting technologies (such as CRISPR/Cas9) as they allow for gene delivery in an efficient...Expression in Mammals A Match Made in Heaven: CRISPR/Cas9 and AAV Viral Vector Elements Additional Resources...Ready-to-Use AAV Preps Browse All of Our Viral Plasmids and Resources ...

  16. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...), 339-350 (2015). PubMed. Find the Plasmids @Addgene: Plasmids for CRISPRi are described here: http...) model consists of a catalytically dead Cas9 protein (dCas9) that lacks endonuclease activity coupled...constructs to be introduced simultaneously in a single plasmid. The functionality of the RNA scaffolds follows...http://www.addgene.org/crispr/qi/ Plasmids described in  Zalatan et al. Cell  (2015) can be found here...https://www.addgene.org/browse/article/9593/ Plasmids described in Qi et al. Cell  (2013) can be found.... Using dCas9 as an on/off switch The multiple scRNAs all individually utilize the same dCas9 protein....modulate dCas9 expression, multiple scRNA transcriptional programs can be regulated with dCas9 as a master...

  17. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...CAPTURE 2.0 to be used with primary cells. Find plasmids for CAPTURE 2.0! When compared to the original...1st time CRISPR users Learn how to Choose the Best Cas9 Variant for Your Next Experiment Learn More About... Additional Resources on Addgene.org Find Other Cas9 Variants for Purifying Genomic Regions of Interest...co-expressed, the gRNA targets the dCas9 to the loci of interest and dCas9 is biotinylated by BirA. From here...To increase the resolution, the Xu lab created a dCas9-based CAPTURE (CRISPR Affinity Purification in situ... cell line that co-expresses three components: dCas9 with an added biotin acceptor site BirA, a biotin...be pulled down via streptavidin IP. Biotinylated dCas9 goes along for the ride throughout all of these ...

  18. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...from single or multiplex CRISPR-Cas9 double-strand DNA breaks using SpCas9. More features will be added ...Currently, ICE is configured for Cas9 fromStreptococcus pyogenes (SpCas9). ICE Score - The editing efficiency...Addgene.org Check out our CRISPR guide Find CRISPR plasmids for your research Find other CRISPR software resources...Addgene Blog Browse our CRISPR featured topic page xCas9: Engineering a CRISPR Variant with Pam Flexibility...

  19. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...over 100 requests for the plasmids and the lab eventually deposited these plasmids at Addgene. While deposits...The mission to share critical reagents such as plasmids and viral stocks for researchers around the world...Therapeutics and is an Addgene depositor. KSQ uses a CRISPR-Cas9 platform to systematically test the function of ...

  20. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...used with many types of proteins, including Cas9 and dCas9-transcription factors, for which low expression...light-responsive system is spread across three plasmids, with the response regulator encoded in the genome...et al. condensed the system to two streamlined plasmids. The utility of these TCSs was limited by a few...These bulky systems were spread over a number of plasmids, with one RR (ompR) encoded chromosomally. Leakiness...constructs have been greatly streamlined; only two plasmids are required per system. Applications of these...many strains of E. coli, as well as with other plasmid-based systems, which will enable the assembly of...sb500273n. Epub 2014 Oct 11. PubMed. Find the plasmids from this publication at Addgene. Synthetic biology...
Showing: 181 - 200 of 284 results