Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 636 results for: erf

Showing: 181 - 200 of 636 results

  1. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ...have taken the CRISPR world by storm. Like RNA interference, these enzymes can knock down RNA without altering...nuclease domains characteristic of the Cas13 superfamily. Yan et al. proceeded to study orthologs from...multiple RNA regulating methods: small hairpin RNA interference, dCas9-mediated transcriptional inhibition (...

  2. Hot Plasmids - February 2022

    Type
    Blog Post
    ...-based method for screens. Cas12a’s ability to perform multiplex editing within a single expression cassette...used to create a genome-wide library capable of performing similarly to Cas9 screens in a compacted library... via a nuclease-active CasMINI. This small yet powerful system opens up new possibilities in both cell...

  3. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ...couple of promising variants to check how they perform under your experimental conditions. This is time...combinations of biology and equipment. Because the performance of a fluorescent protein strongly depends on ...endogenous tagged genes) can be used to verify performance under different imaging conditions (e.g. different...

  4. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...target DNA. Conventional thermal cycling is then performed to amplify the target using PCR. Instead of tracking...mutation status. The drop-off probe is designed to perfectly complement the wild type sequence and is not capable...design such assays, in addition to testing their performance (2). Remember that although digital PCR is a ...

  5. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    ...but it doesn’t really matter if those overhangs perfectly match up. They just need enough complementary ... To overcome this limitation, one option is to perform a hierarchical assembly, assembling fragments in...Choosing a Molecular Cloning Technique Choosing Your Perfect Plasmid Backbone ...

  7. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ... forces scientists to use invasive measures to perform in vivo optogenetic experiments. Intracranial injections...correlated with the model’s predictions.  Three top performers, named ChRger1, ChRger2, and ChRger3, were then...minimally-invasive optogenetics While ChRgers performed well in a tissue culture dish, that doesn’t guarantee...

  8. To Each HIS Own

    Type
    Blog Post
    ...step to remove the tag in order to prevent it interfering with protein function. In contrast, the polyhistidine... it is, and the greater the risk that it will interfere with protein function.  Additional Benefits of...this one developed by Dr. James Trimmer’s lab, to perform assays that they otherwise would not be able to...

  9. All in a Twist: dsRNA

    Type
    Blog Post
    ...stranded? Well, that’s just for DNA – right? RNA performs almost all of its biological functions in our ...sense dsRNA and respond accordingly. While not perfect, these sensing systems work to distinguish between...blog CRISPR 101: RNA Editing with Cas13  RNA Interference in Plant Biology  ...

  10. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ... from Addgene’s repository that can be used to perform screens in human cells. General information regarding...Liu at Oxford University, we are now providing a powerful CRISPR-based screening source for the Drosophila...resources for fly labs who wish to harness this powerful genome engineering tool. Bassett, et al. J Genet...locus causes that locus to be methylated. This powerful tool has been used to direct methylation to and... that high-throughput tagging projects can be performed. Schwartz, et al. Genetics. 2016. PubMed PMID:...

  11. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...cloning strategy designed by synthetic biologists to perform large scale, one-tube cloning reactions. In this...courtesy of Rob Hurt. What makes MoClo systems so powerful is their modularity — many parts can be assembled...used to assemble multigene constructs capable of performing production and subsequent necessary modification...

  13. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...specific to lasers or optogenetics so cheaper, less powerful alternatives to the Master-9 that only control...published first. Because behavioral optogenetics is performed in freely moving animals, the fiber optic patch...into some of the important considerations when performing optogenetics behavioral experiments. Thank you...

  14. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ... capture of chromatin interactions The Xu lab performed a proof-of-concept experiment to show that CAPTURE...original CAPTURE method. Both CAPTURE methods outperformed other 3D chromatin methods such as ChIA-PET ...Wang, K. (2018). 3C and 3C-based techniques: the powerful tools for spatial genome organization deciphering...

  15. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    ...consider Gibson cloning. In this technique, you first perform a round of PCR to generate DNA fragments with 15...function while 5’ adenosine overhangs left by Taq perfectly anneal with the topoisomerase I generated overhangs...available cloning kits that one could choose from to perform these experiments. In most cases, there will be...

  16. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ... have a much better chance of creating a high-performing team - which is every manager’s dream. Learn ...often associate feedback with end-of-the-year performance review, but feedback should be given throughout...the year and in a timely manner. If someone’s performance or behavior is inadequate, you need to address...

  17. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ... here!). The only issue with these GEVIs? They perform poorly in two-photon conditions, which are necessary...the fluor via FRET. This system is particularly powerful for in vivo imaging especially since JFs can cross...type of interest. Viral pseudotyping is also a powerful tool that swaps capsid proteins from different...

  18. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...thousands of designs in parallel. To do this, we performed high-throughput ‘self-targeting’ screens with ...PRIDICT2.0 score. For the best prime editing performance, we suggest that users select some of the best...learning–based model with high generalization performance. Science Advances, 5(11), eaax9249. https://doi.org...

  19. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...One of the most powerful strategies to investigate a gene's function is to inactivate, or "knockout",...arms. When we select cells later, we will first perform a positive selection for the antibiotic resistance... knockout and new CRISPR tools continue to be perfected, the production of cells and mouse knockout lines...
Showing: 181 - 200 of 636 results