...signaling molecules, as some buffer components can interfere with the conjugate or conjugation process. Sodium...used as an antimicrobial agent. Other chemical interferences with conjugating reactions are possible. It ... antibody to specifically bind to a protein is powerful, but in order to utilize it in the lab, researchers...fluorophores. Horseradish peroxidase (HRP) HRP performs a chemiluminescent reaction in the presence of...activated and its output measured. This method can be performed using the indirect detection method via a conjugated...protein complexes for downstream analysis. They are powerful tools for understanding protein or protein interactions...above. Reverse the crosslink, isolate DNA, and perform downstream analysis. Special considerations One...

...within the genome. In a perfect world, your gRNA target sequence would have perfect homology to your target...Design gRNA Publications Get Started CRISPR is a powerful system that enables researchers to manipulate ... particular model organism. There may not be a perfect plasmid for your specific application, and in such...can make a limited set of mutations. Repress or Interfere (CRISPRi) Reduce expression of a particular gene...nucleotide at the same position in spite of being a perfect match for the target sequence. Many gRNA design...

...scalable genome editing technologies, making CRISPR perfect for genome-wide screens. This guide will provide.... Ideally, a gRNA targeting sequence will have perfect homology to the target DNA with no homology elsewhere...compatible with truncated gRNAs to increase specificity SuperFi-Cas9 - increases fidelity with reduced nuclease...for sequences > 10 kb! Currently, these systems perform best in bacterial species, but newer versions engineered...More robust transcriptional repression (CRISPR interference, or CRISPRi) or activation (CRISPRa) of downstream...chromosome. Browse Plasmids: Activate , Repress/Interfere Epigenetic Modifications Using CRISPR A variation...transcription of a target gene CRISPRi CRISPR Interference; using a dCas9 or dCas9-repressor with a gRNA...

... Pooled Libraries Pooled libraries represent a powerful tool for forward genetic screening, or identifying...in the selection NEGATIVE screen: Apply library Perform Next Generation Sequencing (NGS) on a control sample...library Apply selection; most cells will survive Perform NGS on the surviving cells Compare NGS results ...

... (from Chloromonas oogama ), and SdChR (from Scherffelia dubia ). Synthetic variants have been created...Chloromonas subdivisa CheRiff Channelrhodopsin from Scherffelia dubia Channelrhodopsins: cation channels- chimeras... opsin is reached. This approach is especially powerful when combined with site specific recombinase technology...

...with purified enzymes, it takes advantage of the powerful recombination abilities of yeast. Similar to Gibson...than 10kb. Another advantage is the ability to perform oligonucleotide stitching, in which pieces of DNA...

... in gene therapy research to reduce promoter interference and/or drive high expression of a transgene ...

...respect to the precise nature of experiments being performed, and your biosafety office can provide more information...

...Plasmids 101: Antibiotic Resistance Choosing Your Perfect Plasmid Backbone 6 tips for analyzing and troubleshooting...