...in a loss of fluorescence, which, in its simplest form, can be described by a mono-exponential decay (analogous...predict how fluorophores perform in ‘real experiments’, it is advisable to perform time-lapse imaging of ...To determine photostability, an experiment is performed that measures fluorescence intensity over time...Therefore, photostability measurements can be performed with soluble fluorescent proteins or localized...photostability measurements reported in the literature are performed in different ways. We describe a number of issues...used to make these measurements and hope this information can help guide your understanding of reported...fluorescent protein. Photostability measurements can be performed on purified fluorescent proteins. To avoid diffusion...