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Showing: 181 - 200 of 960 results

  2. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...N-Cas9. The N- and C-Cas9 fragments then dimerize to form a functional protein due to being fused with high-affinity..., dCas9 is released within minutes and red foci formed 20-40 min later. The CASANOVA system is versatile...combinations to optimize both fusion proteins. The best performing combination was NLS-dCas9-trCIB1 and NLSx3-CRYPHR-p65...the catalytic site of AcrIIA4, this unfolding conformational shift can interfere with activity. The resulting...

  3. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...good secondaries for cases where you need to pre-form primary-secondary antibody complexes before applying...determines what secondary antibody you should use when performing indirect immunoassays. The Fab regions, on the... is fairly easy to find these in a Fab fragment format. Reporter-conjugated anti-IgG Fab fragments make...applying them to samples, such as when performing IHC in a sample where your secondary antibody recognizes...considerations will help you narrow down which antibody format to use for your specific scenario.    References...

  4. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...0.27 to 4.6 per 10,000 people globally. Pustules form from the uncontrolled activation of the interleukin...will use Addgene’s RESOLUTE plasmid collection to perform a functional screen in mammalian cells to identify...cells with both human and murine targets thus informing potential for success in a patient-mutation bearing...

  5. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...from sharp equipment are one of the most common forms of lab accidents, so extra precaution should be ...

  6. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...cleavage. Instead of cleaving DNA at a defined site to form a clean DNA break, Cas3 locates its target sequence... many genome engineering endeavors. For more information on Cas9 variants, check out our blog post on ...specifically degrade target viral RNA. For more information on Cas13, check out our blog on RNA editing. ...

  7. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    ...Gronostajski who each are being awarded free plasmids form Addgene to accelerate their research. Read on to... of Glycosylation (APCDG) and an international platform for CDG research, named the CDG&Allies – Professionals...guanidinium cation (Gdm+) binding in the location formerly occupied by the Arg sidechain. Although not related...

  8. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...including imaging.  Diabodies are ~60 kDa proteins formed by two Fab fragments bound by short peptide linkers...When Placed in a Divalent Tumor Antigen Binding Format. Journal of Biological Chemistry. https://doi.org...

  10. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...in a loss of fluorescence, which, in its simplest form, can be described by a mono-exponential decay (analogous...predict how fluorophores perform in ‘real experiments’, it is advisable to perform time-lapse imaging of ...To determine photostability, an experiment is performed that measures fluorescence intensity over time...Therefore, photostability measurements can be performed with soluble fluorescent proteins or localized...photostability measurements reported in the literature are performed in different ways. We describe a number of issues...used to make these measurements and hope this information can help guide your understanding of reported...fluorescent protein. Photostability measurements can be performed on purified fluorescent proteins. To avoid diffusion...

  11. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ... for peer learning and knowledge sharing, in the form of workshops, presentations, and lectures. There...with next-generation sequencing data and the bioinformatics pipeline. It’s possible that we’ll find something... have members looking at how to re-use it as a platform for monitoring homemade microfluidic chips using...using the fantastic optics and fluidics platform it contains. Biotech equipment becomes obsolete amazingly...

  12. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...review and most create useful online content in the form of blogs or articles. Look for them on Twitter, ...for free.  Take advantage of this to get the information you need to choose well. What are their qualifications...the desired outcomes? Ask openly for clear information on costs. Can you suggest some coaches? Yes ...

  13. AAV for Neuronal Tracing

    Type
    Collection
    ...fill out our form to let us know what you would like to see available in viral prep format. Please note... protein, the virus can replicate but the newly formed viruses are unable to infect any neighboring cells... AAV can be used with modified rabies virus to perform retrograde neuronal tracing. Note : Addgene does...

  14. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...proteins produced by bacteria become insoluble, forming inclusion bodies that are difficult to extract ...expression vector that is then transformed into a bacterial cell. Transformed cells propagate, are induced...post-translational modifications including protein folding, formation of disulfide bridges, glycosylation and acetylation...

  15. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...monoclonal antibodies (mAbs) and their recombinant forms (rAbs). mAbs and rAbs have higher homogeneity than...non-target proteins), variability (separate batches can perform differently – happens when the antibody is produced...binding to off-target molecules), reliable (wouldn’t perform differently over time), compatible with the desired...images similarly to the negative control, that informs us that the primary antibody is very weak or with...the hippocampus compared to other regions, that informs us that this antibody has specific binding and ...

  16. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...cells. Now, Francois St-Pierre’s lab developed a new form of FACS-based cell screening, Single-cell Phenotypic...optogenetics. Optogenetic tools use light to induce conformational changes in a protein.    Cells expressing...reduced scale in screens without any reduction in performance compared to larger genome-wide libraries. Find...

  17. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ... by blogging. Writing a blog is not only a great form of scientific outreach, it also helps you to hone...talk – to win the audience’s applause with your performance. When your institute opens its doors to the public...cover their annual meetings. 8) Training – Get a formal education Some people get into science communication... Get your community involved in sampling or bioinformatics analysis or upload your data to Zooniverse....

  18. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...not occur or be detected even if the two proteins form a complex. To help troubleshoot your experimental...protein and are useful when a process affects the conformation of the probe. More on these biosensors later...would further increase the amount of correlated information available from a cellular process. FRET experiments...

  19. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...cautioned the distinction of “higher” and “lower” life forms as we shared millions of years of common ancestry...findings from structural studies on the ribosome performed in his lab. RNA is a structural and functional...that RNA in the ribosome is in a complicated conformation “not like DNA which has a boring structure.”...
Showing: 181 - 200 of 960 results