We narrowed to 267 results for: gnal

1. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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   Blog Post

   ...molecule. A higher brightness increases the detected signal and therefore high brightness is an important feature...

2. Multiplex Genome Editing with CRISPR-Cpf1

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   Blog Post

   ...endonucleases, like Csy4, or the inclusion of processing signals, such at tRNAs, to process the array into functional...

3. Screening for Successful Genome Editing with Digital PCR

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   Blog Post

   ...individually assessed for a positive or negative (digital) signal at the end of the reaction (Figure 1). Partitions...

4. Why and how to start studying chromatin (with Addgene's help)

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   Blog Post

   ...complex to chromatin after T lymphocyte receptor signaling. Zhao K, Wang W, Rando OJ, Xue Y, Swiderek K, ...

5. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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   ...CasRx and AdmCas13d fused to a nuclear localization signal (NLS). In a HEK293 mCherry reporter assay, CasRx...

6. Viral Vectors 101: Viruses as Biological Tools

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   Blog Post

   ...They accomplish this through nuclear localization signals which can traffic their viral particles inside ...

7. Hot Plasmids - February 2022

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   Blog Post

   ...can recruit multiple frankenbodies to amplify the signal.  Liu et al. Genes Cells. 2021. https://doi.org...

8. Antibodies 101: Monoclonal Antibodies

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   Blog Post

   ...ligand is bound, then you’ll get a false negative signal - the antibody can’t recognize the protein even...

9. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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   Blog Post

   ...transparent and rigorous studies will give false signals from time to time. False positives in original ...

10. Hot Plasmids - May 2022

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    Blog Post

    ...The researchers validated their work with the “signalome,” a 12-plex mixed-subclone system using mStrawberry...

11. Top Requested Lentivirus and AAV of 2016

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    Blog Post

    ...investigate the effects of activating particular signaling pathways in specific cell types (typically neurons...

12. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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    Blog Post

    ...targeted to the cytosol when expressed without a signal sequence and to the mitochondria via citrate synthase...

13. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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    Blog Post

    ...feedforward loop (iFFL). In general, iFFLs use an input signal that both positively and negatively controls the...

14. CRISPR 101: Cas9 vs. The Other Cas(s)

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    Blog Post

    ...particularly useful in generating transient edits of signaling molecules (RNA) as opposed to permanent edits ...

15. Plasmid Tools for Microbiome Studies

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    ...translation of the reporter gene resulting in GFP signal. With the addition of standards, the assay is semi-quantitative...

16. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    Blog Post

    ...receptor. However, in some cases the fluorescent signal may be critical for the experiment. Since the fused...

17. Antibodies 101: Affinity Reagents

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    Blog Post

    ... extremely small at 12-15 kD but can have weak signals due to their monovalent nature. They are stable...

18. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    Blog Post

    ...bright, exhibits good localization, has high signal:noise ratio, high sensitivity, responds quickly, is...

19. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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    Blog Post

    ...increased expression of transforming growth factor-β signaling, possibly inducing the secretion of growth promoting...

20. CRISPRainbow and Genome Visualization

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    Blog Post

    ...dynamics throughout the cell cycle. To optimize the signal to noise ratio of the system, they modified GFP-dCas9...