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  1. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...of interest is essential, a true knockout can be lethal, and you'd instead want to create a conditional...depositing with Addgene! Figures were adapted from: Hall B, A Limaye, & AB Kulkarni. (2009) "Overview: Generation...

  4. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ... Biology (Kimball et al., 2020). Benefits and Challenges CUREs inspire students to take ownership of their...experience on their resumes). CUREs are not without challenges. Real research is messy, and it can be difficult...

  5. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...samples in a given state or point in time. You want to halt degradative processes while maintaining the structure...fixation-artifacts-and-how-to-minimize-them/  Goldenthal KL, Hedman K, Chen JW, et al (1985) Postfixation...

  6. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...3) they have limited toxicity. However, a key challenge of using AAVs for neuroscience research is the...the Gradinaru Lab at Caltech stepped up to the challenge. Find the PHP Plasmids Here! Their Solution: ...

  7. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...generating the standard curve for qPCR can be challenging to get right. Here at Addgene, we use a linearized...droplet digital PCR for vaccine evaluation in challenged monkeys and TB diagnosis." Emerging microbes ...

  9. CRISPR Antimicrobials

    Type
    Blog Post
    ...may provide a method for doing just that. While challenges remain in the delivery of these agents, CRISPR...CRISPR-induced double-stranded break (DSB) in the genome is lethal. If this DSB occurs on a plasmid, the plasmid will...

  10. Plasmids 101: Protein tags

    Type
    Blog Post
    ...purify proteins can face any number of technical challenges depending on their protein of interest. After...After several decades of trying to address these challenges, researchers have amassed a considerable molecular...

  12. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...transgenic hairy root lines in tissue cultures takes half a year, and generating a transgenic plant can take...Luciferases usually have shorter mRNA and protein half-lives then GUS and are therefore good temporal reporters...

  14. Lab to Office Culture Shock

    Type
    Blog Post
    ...counter-intuitive, but I find office work more mentally challenging than lab work at times.” Sometimes a desk job...to non-scientists can be a fun and gratifying challenge. You’re also getting the opportunity to learn ...

  15. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...relationships. Personally, I find this to be the most challenging and time-intensive part of relationship building...research-intensive careers. In addition, this skill is a hallmark for jobs focusing on: Science Policy, Business...

  16. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...antibodies. Assay development, however, can be challenging since scientists must first identify two independent...Longer protocolIncreased chance of cross-reactivityChallenging to develop assay Competitive Appropriate...

  17. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...earlier Voltron indicators (a fusion of Ace2N and HaloTag, Figure 4), they determined that an A122D mutation...changing the dye that becomes conjugated with its HaloTag domain.  Find Voltron2 plasmids here! Abdelfattah...

  18. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...from flow cytometry experiments can be a little challenging to understand if you’ve never done any flow. ...., Sait, K., Anfinan, N., Al-Wasiyah,  mohammad khalid, Huwait, E., Gari, M., & Al-Qahtani, M. (2018)....

  19. Bioinformatics at Addgene

    Type
    Blog Post
    ...300,000 individual nucleotide sequences. More than half of these sequences were generated by Addgene as ...on-site, but now we were presented with a new challenge: automating the bioinformatics steps needed to...

  20. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...suited for recombinant protein expression. Many challenges can arise when over-expressing a foreign protein...functional RNaseE which results in longer transcript half-life F- ompT lon hsdSB (rB- mB-) gal dcm rne131...
Showing: 181 - 200 of 429 results