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  1. Gibson Assembly Protocol

    Type
    Protocol
    ...part, one part has half of the antibiotic gene and the adjacent part has the other half. Any colonies should...

  2. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...medium at a reduced antibiotic concentration (e.g., half the concentration that was used for the initial ...genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nature Methods. 2014 Aug;11(8):783-...

  3. Weighing Reagents Protocol

    Type
    Protocol
    ...Pro-Tip If you’re using weighing paper, fold it in half first to create a crease and then place it unfolded...

  6. AAV Production in HEK293 Cells

    Type
    Protocol
    ... until fully dissolved. Pro-Tip This step is challenging due to the high viscosity of PEG. Stirring under...

  7. CRISPR Library Amplification

    Type
    Protocol
    ...Quality Control (QC) Pooled libraries can be challenging and expensive to ensure adequate quality, but...
Showing: 1 - 7 of 7 results