Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 297 results for: hal.2

Showing: 181 - 200 of 297 results

  1. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...retain their individual activities when separated (2), and that DBD and TAs from different systems could... Enzymol. 1983;100:293-308. PubMed PMID: 6312261. 2. Keegan, L., Gill, G., and Ptashne, M. Separation ...functional luciferase only when fusions of the two halves of the luciferase protein interact and enable intein...

  2. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...of these neurons via the channel Channelrhodopsin 2 (ChR2) reduced glutamate transmission from the mPFC...deliver Cre-dependent expression of  Channelrhodopsin 2 (ChR2), a modified receptor protein that activates...stay tuned!  References Alpár, Alán, et al. "Hypothalamic CNTF volume transmission shapes cortical noradrenergic...

  3. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ... ligands can elicit varying degrees of β-arrestin-2 recruitment to receptors, indicating their agonistic...fluorescent labels. In Nature Methods (Vol. 19, Issue 2, pp. 149–158). Nature Research. https://doi.org/10.1038...interchangeable labeling using the HaloTag® system, the new Janelia Fluor HaloTag Ligands expand the self-labeling... self-labeling proteins would be SNAP-tag® and HaloTag®. These consist of a genetically encoded enzyme...Hayashi-Takanaka et al., 2014). Other dyes may prove challenging to stain intracellular compartments in in vivo...2017). These dyes were adapted into ligands for HaloTag® and are currently offered at Promega. Due to the...

  4. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...six different research constellations: Doench/Root (2), Zhang (3), Wu (4), Yusa (5), Wang/Lander/Sabatini...Bioinformatics 33.7 (2016): 1099-1100. PubMed PMID: 28414855. 2. Doench, John G., et al. "Optimized sgRNA design to...that code for surface proteins. One of the first challenges with a custom screen approach is to identify ...PMCID: PMC4744125. 3. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide...essentiality profiling reveals gene networks and synthetic lethal interactions with oncogenic Ras." Cell 168.5 (2017...

  5. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...Almo, Institute for Protein Innovation  1:18:45 - 2:01:18 (end) - Panel Alex Tucker (Ginkgo Bioworks)...their curation of citable, linkable figures. Cause #2: Hidden “negative” data We all want to be able to ...Pamela Hines (Senior Editor at Science) Edward J. Hall (Professor of Philosophy at Harvard University) ...experiments they’re reading about weren’t changed halfway through in order to chase a positive result. Beyond...

  6. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    ...), 1668. https://doi.org/10.1038/s41467-019-09656-2 More resources on the Addgene blog CRISPR101: Validating... cancer immunotherapy targets but are embryonic lethal, hindering studies post embryogenesis. Using C-... in a developing or developed immune system was lethal (LaFleur et al., 2024).   Figure 1: Schematic...

  7. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    ...motion videos is very time consuming; producing a 1-2 minute video takes an entire week of our free time...cycle of one step. Challenges in the LEGO science video process One major challenge when we started was...will bring people together and make these daily challenges a little easier to overcome. We want to encourage...

  9. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...short-read sequencing are highlighted in Figure 2. Figure 2: Overview of short-read sequencing technology... up the pace Our QC team constantly faces the challenge of keeping up with the large amount of QC analysis...

  10. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...’re delivering their payload to.   Figure 2: Numbers of AAV tools, by category, available from... substrates, light, or engineered ligands (Figure 2). Biosensors, which include calcium and voltage sensors...depending on their serotype, are particularly challenging. Their efficacy in any given system can be affected...

  11. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...sequence is unique compared to the rest of the genome.  2) The target is present immediately upstream of a Protospacer...utilized allowing for PAM flexibility. Fig. 2: Prime editor engaging target DNA. The reverse transcriptase...essential genes whose knockout would cause cell lethality. The Krüppel associated box (KRAB) is a naturally...

  13. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    ...Transgenesis." PLoS ONE. 8(12): e83043 (20 December 2013). 2. Carola Engler et al. "A One Pot, One Step, Precision...practical terms, what GreenGate can do is “save you half a year,” in comparison to more tedious and time-...

  14. Let There Be LITE Plasmids

    Type
    Blog Post
    ...First, the light-responsive molecules of cryptochrome 2 are the chromophores flavin-adenine dinucleotide and...and CIB1 – which they borrowed from Arabidopsis thaliana. The results are light-inducible transcriptional...

  15. Plan Your Experiment

    Type
    Guide
    ...components (Figure 2), and the optimal system will likely depend on your cell type. Figure 2: Different delivery...either (1) a lack of gRNA and/or Cas9 expression or (2) a lack of efficient target cleavage in cells that...CRISPR-Based genome editing. Trends in Biotechnology , 36 (2), 173–185. https://doi.org/10.1016/j.tibtech.2017.11.006...generally higher than HDR pegRNA optimization can be challenging Prime Edit Repress or Interfere (CRISPRi) Reduce...

  16. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ... both substantial merit and national importance; (2) The foreign national must be well positioned to advance...outstanding achievements in your specific academic field, (2) that you have three 3 years of research experience..., Casseus Law suggests a mix of experts including 2 dependents –people who have worked with you i.e. your...for companies to submit an application on their behalf.  If an individual obtains a cap subject H-1B (...

  17. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...- Bacterial Expression mScarlet-I3 568 592 68 4.2 2 min Monomer pmScarlet-I3_C1 - Mammalian Expression...Maturation is the time for fluorescence to obtain half-maximal value. Structure describes the monomeric...

  18. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...similar optical quality (magnification of 140X and 2 micron resolution). I have worked many hours in a ...University). Right there on the floor outside the lecture hall. It was wonderful to see the ad-hoc ‘sciencing’ ...

  19. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    ...much has been done with CRISPR technology in just 2 years and excitement about how much more there is ...systems, modifications to the RNA components, and challenging target genomes, such as the malaria-causing Plasmodium...Addgene depositor, discussed some of the many challenges of creating biological circuits, and I can only...
Showing: 181 - 200 of 297 results