We narrowed to 277 results for: mel

1. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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   ..., X. laevis (frog), D. rerio (zebrafish), D. melanogaster (fly), C. elegans (nematode), S. cerevisiae ...

2. Fluorescent Proteins 101: Introduction to FRET

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   ... The first genetically encoded FRET biosensor, Cameleon (Miyawaki et al., 1997), was designed to measure...

3. Plasmids 101: Modular Cloning

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   ...combinations. Even with current technologies, it can be extremely hard to predict how a single TU, or even an individual...

4. Hot Plasmids and Viral Preps - January 2021

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   ...24390935/ Hypercompact genome editor CRISPR-Casɸ By Melina Fan The Doudna Lab adds to the CRISPR-Cas toolbox...

5. Hot Plasmids - October 2020

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   ...targeting Cas system that can be used in Drosophila melanogaster. Get the plasmids at Addgene The Cas Hybrid...

6. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

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   ...your action item list and set some approximate timelines. Be sure that you keep your commitments, and if...

7. PCR Applications for Copy Number Variation Assays

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   ...not need to be run in triplicates. ddPCR is an extremely accurate application, even in areas of the genome...

8. Magnetic Control of Proteins: More than a Dream

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   ...Dodson, C. A., Wallace, M. I., Mackenzie, S. R., Timmel, C. R., & Hore, P. J. (2016). Chemical amplification...

9. Pooled Library Amplifications

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   ...Re-Amplification Protocol (PDF, 71 KB) Barcode Watermelon Library 5,000,000 30 bp At least 200 ng...

10. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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    ...epigenetic modifications, we can start to build a timeline of events that surround changes in gene expression...

11. Academic vs. Industry Postdocs

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    ...afford to spend too much time working on something extremely risky or exploratory. Getting a job afterwards...

12. Plasmids 101: 5 factors to help you choose the right cloning method

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    ...your favor. For starters, Golden Gate cloning is extremely efficient due to the use of the versatile type...

13. Plasmids 101: Protein Expression

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    ... inducible promoters. Inducible promoters are extremely useful if a desired protein product is toxic to...

14. Fluorescent Tagging of Endogenous Genes with SapTrap

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    ...Toolbox for Genome Editing in Cultured Drosophila melanogaster Cells.” G3 (Bethesda) 6(6) (2016):1777-85. PubMed...

15. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    ...line. Knock downs in Drosophila cell lines are extremely effective. Their Snapdragon tool helps you select...

16. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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    ...Replacement by Compound Autosomes in Drosophila melanogaster. Genetics 74, 461–475 (1973). PubMed. More ...

17. Plasmids 101: Multicistronic Vectors

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    ... Melina Fan...

18. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    ...Resources References Hwang, J. M., Piccinini, T. E., Lammel, C. J., Hadley, W. K., & Brooks, G. F. (1986). ...

19. An Introduction to Adenovirus

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    ...targeted cells is less likely for adenoviral vectors – namely due to the removal of certain adenoviral genes ...

20. Viral Vectors 101: The Retroviral Lifecycle

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    ...have their own unusual sort of virulence as well — namely that they can cause cancer via insertional mutagenesis...