Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
Type
Blog Post
... Melina Fan...
We narrowed to 279 results for: mel
-
-
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post...status into account (Fig 1a). The laboratory of Karmella Haynes at Emory recently contributed a set of ... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog Post...MS, Rinaldi C, Slaymaker IM, Yen J, Young LE, Ciaramella G (2020) Directed evolution of adenine base editors... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog Post...authors by exploring author pages (for example, Melina Fan’s author page). These pages show all publications... -
Plasmids 101: Modular Cloning
TypeBlog Post...combinations. Even with current technologies, it can be extremely hard to predict how a single TU, or even an individual... -
Tips for Technical Support Calls
TypeBlog Post...response, however, consider following up. Ask for a timeline if you are not given one. Though an initial response... -
7 Simple Strategies to Resolve Conflicts with Difficult Supervisors
TypeBlog Post...your action item list and set some approximate timelines. Be sure that you keep your commitments, and if... -
Magnetic Control of Proteins: More than a Dream
TypeBlog Post...Dodson, C. A., Wallace, M. I., Mackenzie, S. R., Timmel, C. R., & Hore, P. J. (2016). Chemical amplification... -
Pooled Library Amplifications
TypeBlog Post...Re-Amplification Protocol (PDF, 71 KB) Barcode Watermelon Library 5,000,000 30 bp At least 200 ng... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post...Toolbox for Genome Editing in Cultured Drosophila melanogaster Cells.” G3 (Bethesda) 6(6) (2016):1777-85. PubMed... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog Post...line. Knock downs in Drosophila cell lines are extremely effective. Their Snapdragon tool helps you select... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog Post...your favor. For starters, Golden Gate cloning is extremely efficient due to the use of the versatile type... -
Plasmids 101: Protein Expression
TypeBlog Post... inducible promoters. Inducible promoters are extremely useful if a desired protein product is toxic to... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog Post...epigenetic modifications, we can start to build a timeline of events that surround changes in gene expression... -
PCR Applications for Copy Number Variation Assays
TypeBlog Post...not need to be run in triplicates. ddPCR is an extremely accurate application, even in areas of the genome... -
Academic vs. Industry Postdocs
TypeBlog Post...afford to spend too much time working on something extremely risky or exploratory. Getting a job afterwards... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog Post...Replacement by Compound Autosomes in Drosophila melanogaster. Genetics 74, 461–475 (1973). PubMed. More ... -
Plasmids 101: Multicistronic Vectors
TypeBlog Post... Melina Fan... -
Simplify Cloning with in vivo Assembly
TypeBlog Post...First design the template binding region, with a melting temperature (Tm) of around 60 °C for all primers... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog Post...genome-wide negative selection screens with both A375 (melanoma) and AT29 (colon cancer) cells and assessed the...