We narrowed to 22 results for: mel
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TypeProtocol... 2019 Estimate of time required: ~3 h Workflow Timeline Plate set-up: 2 h qPCR run: 1.5 h Data analysis...°C 30 sec / read plate/ repeat 39x from step 3 / melt curve Example of plate set-up: 1 2 3 4 5 6 7 8 A...accurately determine differences between samples. Melt curve analysis: a single peak should be seen. The...
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What is Polymerase Chain Reaction (PCR)
TypeProtocol...Set annealing temperature 5°C below the primer melting temperature (Tm). Set extension step at 1-2 minutes... 3’ primers should be designed to have similar melting temperatures (Tm). Set the annealing temperature... -
Protocol - pLKO.1 – TRC Cloning Vector
TypeProtocol... Qiaquick gel extraction kit Qiagen #28704 Low melting point agarose Sigma #A9414 Luria Broth Agar (LB...37°C for 2 hours. Run digested DNA on 0.8% low melting point agarose gel until you can distinctly see ... -
Protocol - How to Design Primers
TypeProtocol...60% G/C content Start and end with 1-2 G/C pairs Melting temperature (Tm) of 50-60°C Primer pairs should... -
Ligation Independent Cloning
TypeProtocol...Use web-based primer design software to ensure a melting temperature between 50-60°C for your PCR primers... -
Fluorescence Titering Assay
TypeProtocol... institution’s biosafety regulations. Workflow Timeline Day 0: Seed 293T cells Day 1: Transduce cells ... -
General Transfection
TypeProtocol... and different transfection reagents. Workflow Timeline Day 0: Seed HEK293T cells (or a subclone of HEK293T... -
Immunocytochemistry
TypeProtocol...science. Last Update: January 20, 2022 Workflow Timeline Day 1: Seed cells Day 3-4: Fix and label cells... -
Lentivirus Production
TypeProtocol... such as stable-cell line generation. Workflow Timeline Day 0: Seed 293T packaging cells Day 1 (pm): Transfect... -
Colony Formation Titering Assay
TypeProtocol...cannot be used for later experiments. Workflow Timeline Day 0: Seed and transduce cells Day 2: Replace... -
Transfection for Recombinant Antibodies
TypeProtocol...regulations. Last Update: February 18, 2022 Workflow Timeline Day 1: Seed cells Day 2: Transfect cells Day 3... -
AAV Purification by Iodixanol Gradient Ultracentrifugation
TypeProtocol...column gradient for AAV purification. Workflow Timeline Day 1: Purify Day 2: Buffer exchange and concentration... -
Virus Protocol - Generating Stable Cell Lines
TypeProtocol...be adapted to alternative cell lines. Workflow Timeline Day 0: Seed and transduce Cells Day 2–3 (am): ... -
Plasmid Cloning by PCR (with Protocols)
TypeProtocol...should select an annealing temperature based on the melting temperature (Tm) of the portion of the primer that... -
Coomassie Purity Stain of Recombinant Antibodies
TypeProtocol...in science. Last Update: June 14, 2023 Workflow Timeline Day 1: Run SDS-PAGE and stain gel Day 1 or later... -
Affinity Purification of Recombinant Antibodies with Protein A or Protein G
TypeProtocol...affinity purify recombinant antibodies. Workflow Timeline Day 1: Purify antibody Day 2 or later: Buffer ... -
Western Blot
TypeProtocol...approaches. Last Update: January 24, 2022 Workflow Timeline Day 1: Prepare lysates, run SDS-PAGE, transfer... -
Antibody Validation Using the Indirect ELISA Method
TypeProtocol...area and avoid breathing in the vapors. Workflow Timeline Day 1: Antigen Coating Day 2: Blocking Day 3: ... -
Isolating a Monoclonal Cell Population by Limiting Dilution
TypeProtocol...individual cells with cloning cylinders. Workflow Timeline This protocol begins with a stable cell pool. ... -
AAV Production in HEK293 Cells
TypeProtocol...handle a large number of T-175 flasks. Workflow Timeline Day 0: Seed cells in CS2 Day 2: Seed cells in ...