Skip to main content

We narrowed to 399 results for: nans

Showing: 181 - 200 of 399 results
  1. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...project involved every Addgenie in the company. Our Finance, Business and Legal teams had to pave the way for...export and shipping on dry ice. The Business and Finance Teams continue to facilitate the formation of partnerships...
  2. Antibodies 101: Isotypes

    Type
    Blog Post
    ...classify antibodies: monoclonal, polyclonal, scFvs, nanobodies, and the list goes on. But have you heard of ..., but it’s also found in tissues. IgG is the predominant isotype during a secondary immune response, which...
  3. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    ...one often works with parts in the micromolar or nanomolar concentration range. Reactions to happen under...compartments, or fused to other effectors from nanobodies to fluorescent proteins. So, I hope you will ...
  4. Addgene: The First Twenty Years

    Type
    Blog Post
    ... we could use the same principles to provide recombinant antibodies, another plasmid-based product that... In March 2022, we launched our ready-to-use recombinant antibodies service, part of the Neuroscience ...
  5. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...not a “Fab” fragment, Fvs are often produced recombinantly and engineered to be joined by a linker peptide...conjugation.  Finally, Fvs are the most amenable to recombinant expression and will have the highest tissue penetration...
  6. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...The introduction of antibody fragments, such as nanobodies, has introduced new options for increasing signal... on Addgene.org Find plasmids encoding recombinant antibodies at Addgene  ...
  7. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ... Part 1 of this series). In any case, Cre is a dominant phenotype, while you’ll want the floxed gene to...836. PubMed PMID: 29768166. Zickler, Denise, and Nancy Kleckner. "Recombination, pairing, and synapsis ...
  8. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...setting. Molecular diagnostics for the Zika virus predominantly look at viral load in patient blood and urine... carries out research in synthetic biology, nanomaterials science, and self-assembly for applications ...
  9. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...PMC84922. 5. Ugai, Hideyo, et al. "Stability of a recombinant adenoviral vector: optimization of conditions...Heidelberg, 2001. 291-306. PubMed PMID: 10634178. 8. Murnane, John P., Michael J. Yezzi, and Barbara R. Young...
  10. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...surface and “receiver” cells express an anti-GFP nanobody on the cell surface. When the cells interact, ...fluorophore/antibody pairs. Because the anti-GFP nanobody cross-reacts with BFP, sender cells expressing...
  11. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...Phenol-chloroform extraction removes remaining contaminant proteins and RNase A from the DNA sample. When... This will help to remove proteins and other contaminants from the plasmid DNA. It is also advisable to...
  12. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...several micrograms of target DNA from just a few nanograms of template DNA through several cycles of denaturation...number of µl of dH 2 O equal to the number of nanomoles of DNA times 10. For example, if your lyophilized...
  13. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ... concentrations of iodixanol to separate out contaminants from an impure AAV preparation. 15%, 25%, 40%...R.A., & Samulski, R.J. (2007). Production of recombinant adeno-associated viral vectors for in vitro and...
  14. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...wipes) before and after your work. Keep your contaminants away While it’s likely that you’re the main ...microscope will help you identify bacterial and yeast contaminants. Set up a routine to split cells Don’t be lazy...
  15. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...Figure 1). Through this method, we found key determinants of prime editing efficiency such as the length...efficiencies using sequence features and DNA repair determinants. Nature Biotechnology 2023, 1–11. https://doi.org...
  16. DNA Purification Without a Kit

    Type
    Blog Post
    ...into this mixture to absorb the nucleic acids. Containinants bind cellulose less tightly than nucleic acids...placing the dipstick into a wash solution removes contaminants while nucleic acids stick around. Finally, purified...
Showing: 181 - 200 of 399 results