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Showing: 181 - 200 of 404 results
  1. The Importance of High Titer for AAV Transductions

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    Blog Post
    ...References 1.Clark, K. Reed. "Recent advances in recombinant adeno-associated virus vector production." Kidney...PMID: 11841606. 2. Schultz BR, Chamberlain JS. Recombinant Adeno-associated Virus Transduction and Integration...
  2. R Bodies: Membrane-Rupturing Microscopic Tools

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    Blog Post
    ...F. R. Organization and expression of genetic determinants for synthesis and assembly of type 51 R bodies...trait: Absence of toxicity of R-body producing recombinant E. coli on paramecia. European Journal of Protistology...
  3. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...their first protocol is available now. It is for recombinant expression of phosphoserine-including proteins...Site-specific Incorporation of Phosphoserine into Recombinant Proteins in Escherichia coli. 12(21). https:/...
  4. Antibodies 101: Isotypes

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    Blog Post
    ...classify antibodies: monoclonal, polyclonal, scFvs, nanobodies, and the list goes on. But have you heard of ..., but it’s also found in tissues. IgG is the predominant isotype during a secondary immune response, which...
  5. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...project involved every Addgenie in the company. Our Finance, Business and Legal teams had to pave the way for...export and shipping on dry ice. The Business and Finance Teams continue to facilitate the formation of partnerships...
  6. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    ...one often works with parts in the micromolar or nanomolar concentration range. Reactions to happen under...compartments, or fused to other effectors from nanobodies to fluorescent proteins. So, I hope you will ...
  7. Addgene: The First Twenty Years

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    Blog Post
    ... we could use the same principles to provide recombinant antibodies, another plasmid-based product that... In March 2022, we launched our ready-to-use recombinant antibodies service, part of the Neuroscience ...
  8. Antibodies 101: Fab Fragments

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    Blog Post
    ...not a “Fab” fragment, Fvs are often produced recombinantly and engineered to be joined by a linker peptide...conjugation.  Finally, Fvs are the most amenable to recombinant expression and will have the highest tissue penetration...
  9. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...The introduction of antibody fragments, such as nanobodies, has introduced new options for increasing signal... on Addgene.org Find plasmids encoding recombinant antibodies at Addgene  ...
  10. Mouse Modeling, Part 2: Breeding and Crossing Mice

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    Blog Post
    ... Part 1 of this series). In any case, Cre is a dominant phenotype, while you’ll want the floxed gene to...836. PubMed PMID: 29768166. Zickler, Denise, and Nancy Kleckner. "Recombination, pairing, and synapsis ...
  11. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...surface and “receiver” cells express an anti-GFP nanobody on the cell surface. When the cells interact, ...fluorophore/antibody pairs. Because the anti-GFP nanobody cross-reacts with BFP, sender cells expressing...
  12. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...setting. Molecular diagnostics for the Zika virus predominantly look at viral load in patient blood and urine... carries out research in synthetic biology, nanomaterials science, and self-assembly for applications ...
  13. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...PMC84922. 5. Ugai, Hideyo, et al. "Stability of a recombinant adenoviral vector: optimization of conditions...Heidelberg, 2001. 291-306. PubMed PMID: 10634178. 8. Murnane, John P., Michael J. Yezzi, and Barbara R. Young...
  14. Kit Free RNA Extraction

    Type
    Protocol
    ...sample(s) using a spectrophotometer (such as a Nanodrop), agarose gel, or bioanalyzer. For more information...
Showing: 181 - 200 of 404 results