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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 388 results for: nans

Showing: 181 - 200 of 388 results

  3. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...surface and “receiver” cells express an anti-GFP nanobody on the cell surface. When the cells interact, ...fluorophore/antibody pairs. Because the anti-GFP nanobody cross-reacts with BFP, sender cells expressing...

  4. Mouse Modeling, Part 2: Breeding and Crossing Mice

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    Blog Post
    ... Part 1 of this series). In any case, Cre is a dominant phenotype, while you’ll want the floxed gene to...836. PubMed PMID: 29768166. Zickler, Denise, and Nancy Kleckner. "Recombination, pairing, and synapsis ...

  5. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...setting. Molecular diagnostics for the Zika virus predominantly look at viral load in patient blood and urine... carries out research in synthetic biology, nanomaterials science, and self-assembly for applications ...

  6. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...PMC84922. 5. Ugai, Hideyo, et al. "Stability of a recombinant adenoviral vector: optimization of conditions...Heidelberg, 2001. 291-306. PubMed PMID: 10634178. 8. Murnane, John P., Michael J. Yezzi, and Barbara R. Young...

  8. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...Figure 1). Through this method, we found key determinants of prime editing efficiency such as the length...efficiencies using sequence features and DNA repair determinants. Nature Biotechnology 2023, 1–11. https://doi.org...

  10. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...wipes) before and after your work. Keep your contaminants away While it’s likely that you’re the main ...microscope will help you identify bacterial and yeast contaminants. Set up a routine to split cells Don’t be lazy...

  11. Tips for a 1st time AAV user (by a Rookie AAV user)

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    Blog Post
    ... concentrations of iodixanol to separate out contaminants from an impure AAV preparation. 15%, 25%, 40%...R.A., & Samulski, R.J. (2007). Production of recombinant adeno-associated viral vectors for in vitro and...

  12. Great Results Start with Great Standard Curves

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    Blog Post
    ...sure that the sample is pure and free of any contaminants that could affect the measurements. You'll need...Addgene's Ready-to-Use Antibody collection Addgene's Recombinant Antibody Plasmids collection  ...

  14. DNA Purification Without a Kit

    Type
    Blog Post
    ...into this mixture to absorb the nucleic acids. Containinants bind cellulose less tightly than nucleic acids...placing the dipstick into a wash solution removes contaminants while nucleic acids stick around. Finally, purified...

  15. Tips for arabidopsis transformation

    Type
    Blog Post
    ... plant selection markers (basta, hygromycin, kananamycin, tunicamycin) (Nakagawa et al., 2007). Alternatively...at Stanford University. She studies cell fate maintenance in the stomatal lineage and has made countless...

  16. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ... through Addgene. Anti-V5 [IPI-SV5-Pk1] is a recombinant version of the original V5 tag mouse monoclonal...different antibody-based applications.  IPI’s recombinant version is a fusion of mouse variable and rabbit...

  17. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...-2 research   By Ashley Waldron Ready-to-use recombinant antibodies were recently added to the Addgene...Image from Addgene. These antibodies are recombinant versions of mouse monoclonal antibodies generated...

  18. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...Kalamegam, G., Ahmed, F., Abbas, M., Sait, K., Anfinan, N., Al-Wasiyah,  mohammad khalid, Huwait, E., ...Conventional vs. Flow Cytometry Plasmid-based Recombinant Monoclonal Antibodies Introduction to Antibodies...

  19. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...fluorescent biosensors. Aptabeacons Fluorescence resonance energy transfer (FRET) technology has been studied...PMC4441765. 4. Guo, Peixuan AND Haque, Farzin. RNA Nanotechnology and Therapeutics. Boca Raton: CRC Press, 2014...
Showing: 181 - 200 of 388 results