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We narrowed to 457 results for: nsf

Showing: 181 - 200 of 457 results

  1. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...produces pigment from x-gal (included in the transformation plate medium), turning the bacterial colony...for blue-white screening Use a good control: Transform the backbone plasmid without insert. All colonies...components, and are rescued by genes supplied on the transformed plasmid. Such plasmids may contain genes that...

  2. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ... still in its infancy. A long timeline from transformation to testing and a lack of well-characterized...s made even more difficult by the fact that transformation methods have also been patented. In order to.... We will also be working with open source, transformation technologies like those pioneered by Ewen Mullins...

  3. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...base editor is split into two plasmids and co-transfected into cells for reconstitution. Image from Levy...promoters, CRISPRi parts, selectable markers, transformation acceptor vectors, and sequences for genomic...plasmids using the Cyanogate Kit and subsequent transformation into cyanobacteria. Image from Vasudevan et...

  5. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ...microenvironment. This results in increased expression of transforming growth factor-β signaling, possibly inducing... (micro RNA) and other molecules that can be transferred to other cells, inducing epigenetic changes in.... "Intercellular nanovesicle‐mediated microRNA transfer: A mechanism of environmental modulation of hepatocellular...

  6. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...separate envelope and packaging plasmids as well as transfer plasmids. This concept ensures safe handling and...Gamma-retroviral vectors Popular gamma-Retroviral Transfer Vectors   Expressing your gene of interest pMKO...interest. Lentiviral vectors Popular Lentiviral Transfer Vectors    Expressing your gene of interest pLenti-puro...

  7. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ... the E1 gene from the packaging cell line is transferred into the AdV by recombination. It is therefore...correct recombinant pAdV plasmid is identified, re-transform into a bacterial strain not prone to recombination... adenoviral stock (rAdV-S). HEK293 cells are transfected with the AdV plasmid construct from step #1 and...

  8. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...looks like the genetic cargo in one plasmid (the transfer plasmid), the envelope (Env) gene on a separate... plasmid). Viral production then proceeds by transfection of all three plasmids into a producer cell line...Env, Gag, and Pol genes are not encoded in the transfer plasmid, the component that physically integrates...

  9. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...blot Run your gel and membrane transfer as normal. Immediately after transfer, dry the membrane to maximize...from having to run a whole new gel and membrane transfer. Let’s get started! Before you begin In the best...

  11. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...control. A good positive control is bacteria transformed with the same backbone plasmid. If this control...strain. A good negative control strain is an untransformed culture of the same strain of bacteria you used...Cloning Technique Learn to Perform a Bacterial Transformation Deposit Your Positive Clones with Us ...

  12. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...interactions. In later years, improvements to yeast transformation protocols also helped establish the yeast two-hybrid...applied to the bait in a single population (e.g. transforming both fusion plasmids, or mating two single haploid... haploid yeast fusion plasmid transformants into a diploid strain for screening), or arrayed on a 96 well...

  13. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ...constitutively expressed to identify successfully transfected cells. C-to-T editing by the APOBEC-Cas9 editosome... to cells segregated solely by a reporter of transfection, which only report the efficiency of plasmid...frequency of base edits of interest than solely transfected cells. “Gene On” (GO)- a functional reporter ...

  14. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...using mCherry for selection of sgRNA plasmid transformants, they avoid M. abscessus’ high rate of spontaneous...indels after DNA repair. B) M. abscessus is first transformed with Cas9 on an integrative plasmid containing...resistance selection marker KanR. This strain is then transformed with the sgRNA cassette on a plasmid expressing...

  16. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...sequencing or restriction digest! Then, you can transform or transfect the cells that you want to use for your... reactions are performed, the next step is to transform competent E. coli cells and select the positive...

  17. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...errors like uneven pipetting or irregular membrane transfer. Figure 1: A loading control is a type .... No pipetter is perfect, and proteins may not transfer precisely evenly across the entire blot. So how... in the loading control are due to loading and transfer errors — not to any factors from before the proteins...

  18. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...Measuring local temperature variations and heat transfer at the subcellular level requires a probe with...fast measurement of temperature and local heat transfer. Image reproduced from Lu et al. 2022, under CC-BY-NC-ND...plasmids here!   Lu K, et al. Intracellular Heat Transfer and Thermal Property Revealed by Kilohertz Temperature...

  19. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...Emerging CRISPR/Cas9 editing technologies have transformed the palette of experiments possible in a wide...CRISPR technology into C. elegans has been a transformative innovation for our community. Developments ... genomic locus (CRISPR-ChAP-MS) could further transform the range of experiments imaginable in C. elegans...

  20. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...writers have been curious about the capability of transforming the genetic code of living organisms and its...that Streptococcus pneumoniae bacteria could be transformed from one organism to the other. This is now famed...THE CHEMICAL NATURE OF THE SUBSTANCE INDUCING TRANSFORMATION OF PNEUMOCOCCAL TYPES. The Journal of Experimental...
Showing: 181 - 200 of 457 results