We narrowed to 45 results for: nsf

...and plasmid construction was funded by NSF IUSE 1726932 and NSF RCN-UBE 2119918....

...pOpen-EcoRIM M.EcoRI DNA methyltransferase 165517 pOpen-Eco31IA Eco31IA DNA methyltransferase 165518 pOpen-NotIM...pOpen-NotIM M.NotI DNA methyltransferase 165527 pOpen-Eco31IB Eco31IB DNA methyltransferase 165536 pOpen-PstIM...pOpen-PstIM M.PstI DNA methyltransferase 165538 pOpen-SfiIM M.SfiI 165538 DNA methyltransferase 165547 pOpen-SapIM1...pOpen-SapIM1 M1.SapI DNA methyltransferase 165557 pOpen-SapIM2 M2.SapI DNA methyltransferase 165558 pOpen-EcoRVM...pOpen-EcoRVM M.EcoRV DNA methyltransferase 165577 pOpen-XbaIM M.XbaI DNA methyltransferase 165578 pOpen-NcoIM ...into an expression vector of their choice and transforming into E. coli bacteria. This can help reduce ...an expression vector of their choice and then transform into E. coli for expression and purification (...

...Vector Core Viral Vectors Lentiviral Transfer Vectors Adenoviral Transfer Vectors Resources Kazuhiro Oka and...lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors Within this set of...packaging these transfer vectors and generating new viral vectors. Download the Lentivirus Transfer Vector Production...from the PGK promoter Adenoviral Transfer Vectors The adenoviral transfer vectors contained within this ... of lentiviral transfer vectors you can find reporter plasmids to test whether or not you've efficiently...coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase) pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP...Flp recombinases. Genes can be cloned into these transfer vectors using shuttle vectors pICPIS-EF1 (Addgene...

...Tips for Arabidopsis Transformation Save Time with Transient Plant Leaf Transformations Engineering the Plant...System Plasmids for rapidly assembling plant transformation constructs, based on the Golden Gate method...set of functional parts for assembling plant transformation vectors, compatible with MoClo. Golden Gate...Daniel Voytas 1000000236 MoClo Plant Parts III: Transformation & Genome Engineering Kit Collection of MoClo...MoClo Level 0 parts for plant transformation, including regulatory elements, coding sequences, basic CRISPR...cloning of synthetic operons into chloroplast transformation vectors. Golden Gate (MoClo) Scott Lenaghan...Stuttmann 1000000236 MoClo Plant Parts III: Transformation & Genome Engineering Kit Collection of MoClo...

...envelope, and transfer plasmids... Viral Plasmids Lentiviral Plasmids Lentiviral Plasmids Transfer Plasmids Packaging...a virion) are divided among multiple plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA ...third-generation lentiviral packaging, envelope, and transfer plasmids available at Addgene. Read our Lentiviral...safety. Lentiviral Vector Guide Popular Lentiviral Transfer Plasmids Looking for lentiviral plasmids to express... nef . Can be used with 2nd and 3rd generation transfer plasmids. Bob Weinberg 12251 pMDLg/pRRE 3rd Contains... gag and pol . Can be used with 3rd generation transfer plasmids along with a plasmid encoding rev . Didier...

...service gives you even more options. AAV Transfer Plasmids Transfer plasmids encode the genetic information...Browse AAV transfer, packaging, and helper plasmids in Addgene's repository. Viral...Plasmids Adeno-Associated Viral (AAV) Plasmids AAV Transfer Plasmids AAV Packaging Plasmids AAV Helper Plasmids...vectors, researchers need to use three plasmids: A transfer plasmid (or cis plasmid) containing the transgene...This collection provides a curated selection of transfer, packaging, and helper plasmids that can be used... This table contains a general list of all the transfer plasmids in Addgene's repository. You can search...Biosafety Resources Addgene Protocols General Transfection AAV Production in HEK293T Cells AAV Purification...

...Resonance Energy Transfer (FRET) is a process by which energy is non-radiatively transferred from an excited...energy transfer mCerulean, mVenus, and Amber (mVenus Y67C) constructs for measuring energy transfer to multiple...FLIM) or FLIM-FRET, where the increased energy transfer through FRET reduces the donor lifetime. FRET ...2006) Quantitative understanding of the energy transfer between fluorescent proteins connected via flexible...Koushik et al. (2009) Anomalous surplus energy transfer observed with multiple FRET acceptors mTurquoise2... acceptor for Bioluminescence Resonance Energy Transfer (BRET) . Browse Addgene's Luciferase Plasmid Collection...

... latent transforming growth factor beta binding protein 1 MGC163161 LTBP2 latent transforming growth factor...thyroglobulin AITD3, TGN TGFA transforming growth factor, alpha TFGA TGFB1 transforming growth factor, beta 1 ...TGFbeta TGFB2 transforming growth factor, beta 2 MGC116892, TGF-beta2 TGFB3 transforming growth factor...containing) transforming protein 2 SCK, SHCB, SLI SHC3 SHC (Src homology 2 domain containing) transforming protein...MGC138230 TGFB1 transforming growth factor, beta 1 CED, DPD1, TGFB, TGFbeta TGFB2 transforming growth factor...TGF-beta2 TGFB3 transforming growth factor, beta 3 ARVD, FLJ16571, TGF-beta3 TGFBR1 transforming growth factor...protein 2 C14orf141, LTBP3, MSTP031 LTBP3 latent transforming growth factor beta binding protein 3 DKFZp586M2123...

...and transfer plasmids.... Viral Plasmids Gamma-Retroviral Plasmids Gamma-Retroviral Plasmids Transfer Plasmids... been split into multiple plasmids for safety: Transfer plasmid — contains transgene, sgRNA, or shRNA ...collection highlights popular gamma-retroviral transfer, packaging, and envelope plasmids. Read our Gamma-retroviral...Gamma-Retroviral Vector Guide Popular Gamma-Retroviral Transfer Plasmids Want a retroviral plasmid with your gene...of interest? Search our database for retroviral transfer plasmids that have inserts. ID Plasmid 5'LTR Description...

...Bioluminescence Resonance Energy Transfer (BRET) Similar to FRET, energy transfer from the luciferase reaction...white screening can be used for screening of transformants in cloning process. Lynne Postovit 64034 pGL4Luc-RLuc...constitutive promoter can be used to monitor transfection or viral infection efficiency, to study changes... Renilla luciferase is used to monitor viral transfection Feng Zhang 21471 pLenti PGK V5-LUC Neo (w623... expressing firefly luc for monitoring plant transfection. Daniel Voytas 133312 pMKV057 Firefly AtUbi10...and IPT, with firefly luc for monitoring plant transfection. Daniel Voytas 181934 pC0037 Gaussia, Cypridina...

...efficiently transfect. However, note that this method is most useful for cell lines that you can transfect at ...CRISPR system into mammalian cells is to directly transfect expression plasmids encoding your Cas protein ...gRNA, which you generally perform by chemical transfection or electroporation. Expression can be transient...Lentiviral vectors are a good option for difficult-to-transfect cells. You can direct expression to specific cell...These methods are more transient than plasmid transfection or viral vectors, reducing the risk of accumulating...method is helpful in cells that are difficult to transfect, transduce, or may not express common promoters...

...expression vectors, human embryonic kidney cells are transfected with the plasmid(s) encoding the heavy and light... The fluorescence pattern is compared to an untransfected control. Immunohistochemistry (IHC) Tissue sections...extracted from cells, separated by SDS-PAGE, transferred to a membrane, and labeled with the antibody ...antibody. Protein expression is compared to an untransfected control and the protein size is confirmed. Residual...contain low levels of co-purified plasmid from transfection. This residual plasmid is extracted and amplified...

... Material Transfer Agreements Most Addgene orders must be accompanied by a material transfer agreement...agreement (MTA), including transfers to for-profit entities. The MTA is a legal agreement between the recipient... the depositing institution(s). It governs the transfer of biological materials and intellectual property...Please see the Sample Agreements on our Technology Transfer Information page for more information. To learn...

...of the applicable transfer plasmid. We will help you gain legal usage of the transfer plasmid from the ...service lets scientists choose from over 4,000 transfer plasmids to be packaged in their choice of seven...chemogenetic, optogenetic, recombinase, and biosensor transfer plasmids in our repository are eligible for this...several important steps: MTA facilitation for the transfer plasmid, including legal approval and an implementation...

...E2F transcription factor ECT2 Epithelial cell transforming 2 EGFR Epidermal growth factor receptor EIF4EBP... related tyrosine kinase 3 FNT FNTA FNTB Farnesyltransferase, CAAX box FOS FBJ murine osteosarcoma viral...protein 2 ICMT Isoprenylcysteine carboxyl methyltransferase INSR Insulin receptor INSRR Insulin receptor-related... SHC4 SHC (Src homology 2 domain containing) transforming protein SHOC2 SHOC2 leucine-rich repeat scaffold...

...Gate-assembled TALEN, both in microinjected embryos and transfected cells. TALEN expression is driven by the strong...expression in mammalian systems including hard-to-transfect cell lines. Features include (i) Esp3I (BsmBI)...on pTAL7a and pTAL7b for enrichment of double-transfected mammalian cells. In addition, a T7 promoter was...alternative. Based on transient double-selection of transfected cells, the system has successfully been employed...

...reasons: Transfer plasmids with sequence from the 5′ to the 3′ ITR exceeding 4.7 kb Transfer plasmids ... Terms of Purchase and the applicable material transfer agreements (MTAs) and limited use label licenses...

...of viral vectors and find viral production and transfer plasmids for a wide array of research purposes...containing the genetic information to be delivered (transfer or shuttle plasmid) and one or more plasmids containing...capsids (packaging and envelope plasmids). When transfected into in vitro cell cultures, these plasmids direct...

...listing of transfer status for viral preps.... Viral Vector Packaging Service Penn Vector Core Transfer Viral ...Viral Vector Packaging Service: Penn Vector Core Transfer The University of Pennsylvania Vector Core (Penn...inventory , which includes all viral vectors transferred from the Penn Vector Core. Chemogenetics (including...

... educational resources, and efficient material transfer solutions that help advance science while reducing...Promega collection are available in two formats: pretransformed in bacteria as an agar stab 20 µg purified ...NanoBRET (Link opens in a new window) is an energy transfer technique designed to measure molecular proximity...