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We narrowed to 957 results for: omp

Showing: 181 - 200 of 957 results

  1. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...lead to comparable experimental results. Using PiggyBac transposons, the Woltjen Lab compared different...robust engineering. These components are provided in Golden Gate compatible backbones that can be combined...latest hot plasmid updates!     Tol2 Gateway-compatiable toolbox for studying the nervous system The... Cole lab recently deposited the Tol2 Gateway-Compatible Toolobox, which will allow scientists to quickly... neuorodgenerative diseases. The Tol2 Gateway-Compatible Toolbox is based on the original Tol2kit generated...vectors that have standard cloning sites or gateway compatible cloning sites for easy cloning of your genes ...adds new neuronal tools to the expanding gateway compatible vectors currently shared amongst the zebrafish...

  2. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ...with increasingly complex experimental conditions. The ability to construct more complex, multi-fragment...fragments in a single “one-pot” reaction to form complex, multi-insert modular assemblies that enable biosynthetic...as enzyme efficiency, stability, and buffer compatibility have placed practical limits on single- or two-step...enzyme cutting results in 5’ four base overhangs complementary to the overhangs of the assembled inserts. After...assembly Intermediate 5- or 12-fragment assembly More complex 24-fragment assembly Single insert cloning efficiencies...Sequencing of blue colonies showed the expected complete sequence for the lacI/lacZ genes (1), while sequencing...setting up assembly reactions in which a single component was purposefully omitted. Since any assembly is...

  3. Bioinformatics at Addgene

    Type
    Blog Post
    ... Nextflow script. With our completed pipeline, we could adjust the compute resources when needed, for ... number of deposited plasmids increased, the accompanying sequencing data collection and analysis eventually...needed to transform the raw sequencing data into a complete plasmid sequence that would be ready for our QC...data pipeline is to perform several sequential computational tasks for which existing script(s) have been...containers are they are designed to ‘contain,’ in a complete package, all of the pieces of software that depend...is sequestered from other programs running on a computer, so it safely operates in isolation, and can be... be easily shared with others without any complicated setup. We created, tested, and debugged each of ...

  4. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...before vitrification, they can be completely denaturated (left) or complexes may dissociate  (right). B) Protein...determine structures at comparable or higher resolution than using more complex protective methods or detergents...evolved optimizations for prime editing CHARM: A compact epigenetic silencer Recombinant antibodies for ...for low-abundance targets or natively isolated complexes. To address these limitations, the Funabiki Lab...linker histone H1.8 in Xenopus nucleosomes and in complex with its chaperone NPM2, but the technique should...tardigrade LEA, RvLEAMshort, protected samples and was compatible with the buffer additive MgCl2 and the crosslinking...other viral delivery methods, allowing all the PE components to be delivered in a single package. In a recent...

  5. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...tasks that can be accomplished quickly and things that will take longer. Completing the quick and useful...project they’re a part of, completing them will give me some sense of accomplishment. I will prioritize which...its utility) How much time it will take you to complete the task When picking which tasks to actually...will swiftly give you the wonderful feeling of accomplishment while the longer tasks will give you larger...larger payoffs later. Don’t be afraid to accomplish some quick but moderately useful tasks at the expense ...the long and highly useful tasks. The quick accomplishments will likely energize you. For example, let'...experiment. The first way, it will take you 3 days to complete the entire experiment, but you’ll have to spend...

  6. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...user to compare the emission spectra between all of the markers in the panel. This complexity index ranges...choice for overlapping markers. Compensation/reference control(s) Compensation or “reference” controls are...detector for PE. In order to correct for this, compensation has to be applied to “subtract” this spillover...up a small portion of that spectra to use for compensation.     Figure 1: The emission spectra... discern one marker from another. Instead of compensation, which relies on user adjustment, spectral flow...cytometry relies on “unmixing.” Unmixing uses a complex mathematical algorithm and single stained reference...series of histograms flipped onto its side and compressed into a heat map. BV421 peaks in V1, with secondary...

  7. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ... were used to express the necessary components in E. coli (Klompe et al., 2019). Recently, the lab introduced... Sternberg and Zhang labs (Peters et al., 2017; Klompe et al., 2019; Strecker et al., 2019), which direct... a Type I-F CRISPR-Cas system. The four major components required for RNA-guided DNA integration are (... (Fig. 1A, top):  A CRISPR RNA (crRNA) comprising a 32 nt spacer sequence specifying the target site....sequences (R and L ends).  Figure 1: (A) Components involved in each type of CRISPR-Transposon system...multiple Cas proteins to form the Cascade targeting complex is a hallmark of Type I CRISPR-Cas systems, in ...nuclease activity. Instead, it forms a novel co-complex with the transposition protein TniQ for targeting...

  8. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...which can compare up to five editing experiments at a time, and batch analysis, which compares hundreds...edits performed over multiple experiments and comparing the robustness, accuracy, and speed with existing...the accuracy of ICE analysis results was highly comparable (within r2=0.96) to that of NGS data. Advantages... functional KO of the targeted gene.   Analyze Complex Edits   Traditional Sanger sequencing-based analysis...analysis tools are unable to detect or analyze complex edits, such as those generated by multiplexed delivery... How ICE works   Following delivery of CRISPR components into target cells, genomic DNA from both edited...populations is PCR-amplified and Sanger sequenced. ICE compares these sequence traces to give a detailed analysis...

  9. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    ... the “damage” and producing a complete plasmid. Besides being compatible with any vector, the SLIC protocol...overhangs perfectly match up. They just need enough complementary ssDNA to hold the plasmid together, even if ...if there are gaps or flaps remaining. This incomplete plasmid looks like a homologous recombination intermediate...either by annealing a mixture of products or from incomplete PCR — although higher DNA concentrations are ... separate PCR products (middle branch); or by incomplete PCR, which skips the final extension step to ...single-stranded overhangs (right branch). The mixed PCR and incomplete PCR methods yield a minority of usable product...the linearized vector by T4 digestion; then the components are combined and annealed. The resulting recombination...

  10. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...tissue and compare the IHC results to both NeuroMab and the Allen Brain Atlas. The comparison with two ...what you’re comparing your sample to. For example, we use our negative control to compare exposure times...cannot be reproduced and projects that cannot be completed. A 2015 review analyzing and explaining the antibody...reliable (wouldn’t perform differently over time), compatible with the desired applications (such as immunohistochemistry...techniques on rodent brains and is expanding to more complex brain systems such as non-human primates and humans...polyclonal antibodies because of their immunoglobin component that can be produced in large “copies.” This homogeneity...monoclonal antibodies; Addgene collaborates with these companies to create and provide general availability of ...

  11. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...is mediated via individual neurons. Neurons are composed of a cell body, with dendrites receiving incoming...tracing experiments, brain tissue is injected with compounds that intracellularly spread away from the site...distant brain areas. It is desirable for tracing compounds to outline neurons in the direction of information...proteins facilitate transport of neuronal tracing compounds and this is an area that requires further study... neuronal tracing methods Traditional tracing compounds either produce a colorimetric product or are fluorescent...health. Another limitation of traditional tracing compounds is that their diffusion across the synaptic cleft... These methods are less neurotoxic and better compatible with other neuroscience methods such as electrophysiological...

  12. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...more about the company’s program. Once you’ve found a few positions to apply for, compare and contrast ...eventually work at the company at which you’re doing your postdoc? Some companies, such as AstraZeneca,... open up. Other companies, such as Genentech, will not. The point is that each company has a unique approach...people involved is staggering. The process is complicated. If you’re interested in the drug discovery process... publish, but this can vary depending on which company you choose. If publishing is important to you, ...that your project will not involve proprietary compounds or information. Where publication is expected,...meetings. How do I get an industry postdoc? Many companies have industry postdoc positions, including AstraZeneca...

  13. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    ...these components are necessary to efficiently reconstitute a functional viral polymerase complex and utilize...proteins are provided in trans (see Other Important Components of EBOV Minigenome System). However, minigenomes...RNA viruses (reviewed in 2, 3). Other important components of the Zaire ebola virus minigenome system Similar...with the viral polymerase, which is formed by a complex of two proteins: the large (L) catalytic protein...-stranded RNA minigenome. The viral polymerase complex then recognizes the encapsidated minigenome as ... genes in this plasmid would be more difficult compared to if each gene were encoded in separate (and ...particular observation can be easily studied as each component can be examined independently. In addition, experiments...

  14. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...antibody can be used. In a competitive ELISA a multiwell plate is coated with a competing antigen (green).  The...antibodies are used.  Competitive ELISAs Scientists typically use a competitive or inhibition ELISA when...converts into a detectable product. Scientists compare the signal intensity of the sample to that of a... of ELISA are direct, indirect, sandwich and competitive. In a direct ELISA a reporter enzyme conjugated...sample, the less antibody available to bind to the competing antigen. In direct, indirect and sandwich ELISA... level of antigen in the sample whereas in a competitive ELISA the reporter signal is inversely proportional...molecules that may bind non-specifically to assay components and increase background staining. Direct ELISAs...

  15. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...issues in the past, or how completely different teams are tackling comparable conflicts. Is this level ...cooperation: avoiding, competing, accommodating, collaborating, and compromising. Though there may be a...Skills series, this post is not meant to be a comprehensive guide to Conflict Resolution. There are countless...conflict is actually critical in the business world. Companies and organizations that avoid all conflict can ...with whom you strongly disagree, is a critical component of conflict resolution. Having empathy does not...course many other types of conflict resolution. In compromise, you are trying to come to a consensus that all...parties will accept. If you are able to broker a compromise well, you can help everyone walk away from a ...

  16. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...so simple, and engineering complex cellular behaviors requires making complex manipulations to cells. For...assembly. Simply put, it assembles individual DNA components into larger units or circuits with functional...Transcription units (TUs), which are functional units composed of multiple parts transcribed together. They can...and end with a terminator. A simple TU could be comprised of just three parts: promoter, gene, and terminator...MoClo. By a similar principle, TUs can be further complexed into multi-TU constructs, known as multigene constructs...promoters, UTRs, genes, tags, etc.) into MoClo-compatible vectors. Level 1: Assembly of multiple Level ...interact with other proteins and with DNA, and these complexes regulate the transcription of specific genes. ...

  17. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ... homolog of the yeast TREX-2 mRNA export complex: components and anchoring nucleoporin. Lu et al. Plant...generally known as Bimolecular Fluorescence Complementation (BiFC). It can provide a qualitative or quantitative... or even single-particle tracking of the bound complex. There are so many FPs available, with many ways... In several cases an FP(1–10) fragment may be compatible with multiple FP(11) fragments, even from a different...because they are used with an already-available complementary fragment. Next, let's look at a round-up of...protein fragments for bimolecular fluorescence complementation analysis under physiological conditions. Shyu...

  18. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    ...the key components of Nanoblades, how their editing efficiency and off-target effects compare to existing...the following four components into 293T cells. Figure 1: The key components of Nanoblades. Image... primary cells. Comparing Nanoblades to Existing CRISPR Delivery Methods When compared head-to-head with...for HDR editing and mouse-model generation. Key components of Nanoblades Nanoblades are a Cas9 RNP delivery...Nanoblades also had lower off-target effects when compared to DNA transfection of gRNA and Cas9 expression...of RNPs generally having lower off-target edits compared to DNA transfection.  Homology directed repair...helps form DNA and retroviral-particle or VLPs complexes. The team found that "all-in-one" Nanoblades facilitated...

  19. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...Starch-based compostable bags for kit components Recyclable Yes, all kit components and packaging... is completely degraded. Cloning Grade DNA (high quality/low quantity) – You can usually complete a cloning...packaging Yes, most kit components and packaging Yes, most kit components and packaging Parts Sold...can use to test or compare kits before deciding which one to buy. Table 3: Comparison of popular plasmid...a relative term — even armed with the highest-competency cells you will still struggle to get transformants... very similar. However, keep in mind that kit components (i.e. buffers, columns, or beads) are designed...can affect the lysis buffer ratio and result in incomplete lysis. You may be able to scale the amount of...

  20. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...at major companies.  The majority of these meetings acted as a platform for significant company advancement...their 20’s who’ve built multibillion dollar tech companies using only laptops and some server space. But... find an extra layer of difficulty launching a company in the health sciences without those critical three... into commercial products and next generation companies.  We launched in the early spring of 2015 and ...Before even beginning the process of launching a company, you need to determine whether or not your idea...your idea, you will rarely succeed in launching a company without partners.  You need a dedicated team that...funding bodies shy away from one man teams.  The composition of the management team is more important than...
Showing: 181 - 200 of 957 results