... inducible Hsp68 Mammalian Melton 15508 pBS-OMP-CreFNF OMP targeting vector with Cre FNF (neo-selectable...recombination has occurred, allowing for direct comparison of Cre+ and Cre- cells. While Cre-lox recombination...Mammalian Pelczar 51267 pCAG-Co-InCreN N-terminal component of the Co-InCre system CAG Mammalian Pelczar 51268... 51268 pCAG-Co-InCreC C-terminal component of the Co-InCre system CAG Mammalian Pelczar 51273 pCAG-Roxed-Cre...Eisenhoffer 131782 pME-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN, in middle entry ...Eisenhoffer 131785 pAAV-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN, in AAV donor/transfer...Eisenhoffer 131786 pLenti-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN, in lentiviral vector...

...major histocompatibility complex, class I, A FLJ26655, HLAA HLA-B major histocompatibility complex, class...major histocompatibility complex, class II, DO beta DOB HLA-DPA1 major histocompatibility complex, class... Name Synonyms C3 complement component 3 ARMD9, ASP, CPAMD1 C5 complement component 5 CPAMD4, FLJ17816...R33729_1, SF20 C3 complement component 3 ARMD9, ASP, CPAMD1 C3AR1 complement component 3a receptor 1 AZ3B...HNFAG09 C5 complement component 5 CPAMD4, FLJ17816, FLJ17822, MGC142298 C5AR1 complement component 5a receptor...major histocompatibility complex, class II, DM beta D6S221E, RING7 HLA-DOA major histocompatibility complex...

...capture protein:DNA complexes. After capture, the DNA is released from the complex and can be sequenced...is licensed to multiple companies for sale. Before purchasing, directly compare the product information... size comparison of peptides. Independent antibody validation This method validates by comparing your ...configuration of a protein or a protein:protein complex. Due to their increased manufacturing requirements...from antibody fragments (shown in Figure 2) to completely unrelated molecules. Examples include: scFv -...nanostructures. Figure 2: An antibody (IgG, far left) compared to alternative affinity reagents (left to right...conjugating and signaling molecules, as some buffer components can interfere with the conjugate or conjugation...

...detecting multiple genomic loci, and compatible with live cell imaging. Compared to techniques like fluorescence...nuclease pair for every genomic target. Due to its comparative simplicity and adaptability, CRISPR has rapidly...approach. Engineered CRISPR systems contain two components: a guide RNA (gRNA or sgRNA) and a CRISPR-associated...Cas protein). The gRNA is a short synthetic RNA composed of a scaffold sequence necessary for Cas-binding...it meets two conditions: The sequence is unique compared to the rest of the genome. The target is present... protein and the gRNA form a ribonucleoprotein complex through interactions between the gRNA scaffold ...homology with the target DNA. Once the Cas9-gRNA complex binds a putative DNA target, the seed sequence ...

...nuclease form a complex with each individual, unique crRNA. (4) Each crRNA:tracrRNA:Cas9 complex seeks out ...a protospacer adjacent motif (PAM). The CRISPR complex binds to the foreign DNA and cleaves it to destroy...out the DNA sequence complementary to the crRNA. In Type II CRISPR systems, a potential target sequence ...after where the crRNA would bind. (5) After the complex binds, Cas9 separates the double stranded DNA target...strands near the PAM. (6) The crRNA:tracrRNA:Cas9 complex unbinds after the double-strand break. CRISPR systems...classes based on the structure of the effector complex that cleaves the genomic DNA. Type II CRISPR was...following in 2015. Class 1 (Multi-subunit effector complex) Class 2 (Single multi-domain effector) Type I ...

...Determine the reverse complement of each guide sequence. Example reverse complement sequences of the Pim1...repair. Deletions have potential advantages as compared to single-site small indels given the efficiency...oligos for each guide and its associated reverse complement including additional nucleotides for cloning ...sequence and “AAAC” before the guide’s reverse complement for cloning into the pX330 vector using BbsI ...sequence. Add a C at the 3’ end of the reverse complement oligo ( e.g., sgRNA-A in Table 4 ). The resultant...not add C to the final position of the reverse complement oligo. In this case, the resultant oligos would...μl reaction mix for each guide and its reverse complement: 1.0 μl sgRNA 24- or 25-mer oligo (100 μM; see...

... kinase, is found in two complexes, mTORC1 and mTORC2. Each complex contains a unique subunit...tensin homolog Rictor RPTOR independent companion of MTOR, complex 2 SGK Serum/glucocorticoid regulated ... to find available plasmids for the gene. For components with multiple isoforms or subunits, individual...links to each gene page are provided below. mTORC1 Complex Symbol Name 4E-BP Eukaryotic translation initiation... RPTOR; Regulatory associated protein of MTOR, complex 1 Rheb Ras homolog enriched in brain S6K Also known...Hippel-Lindau tumor suppressor Return to top mTORC2 Complex Symbol Name AKT AKT1 AKT2 AKT3 v-akt murine thymoma...

...vectors with various resistances. Comprehensive multi-cloning site compatible with standard cloning using many...redirecting metabolic pathways to produce a therapeutic compound, there are many different ways to alter bacterial...expression of Cas9 nuclease gRNA. For a more comprehensive list of our bacterial CRISPR plasmids, see here...inducing double stranded breaks. For a more comprehensive list of our bacterial CRISPR plasmids, see here...bacterial gene disruption or knockdown. For a more comprehensive list of our bacterial CRISPR plasmids, see here...with an N-terminal 6x His tag and an MBP tag. Compatible with Gateway cloning. pDEST-periHisMBP 11086 ...disulfide bond formation or prevent toxicity. Compatible with Gateway cloning. pGTvL1-SGC 39188 pTac (...

...the New England Biolabs website. For a comprehensive comparison to GFP, please refer to this table. Technology...internal or surface proteins Single constructs are compatible with multiple applications (different fluorophores...allow for dual labeling and visualization of complementary protein function or localization. ACP and MCP...cell surface proteins with chemically diverse compounds. J Am Chem Soc. 126(29):8896-7. PMID:15264811 ...

...designed to specifically localize to cellular compartments or organelles. Addgene’s database includes many...imaging activity in neuronal populations and microcompartments. Nat Methods. 2019 Jul;16(7):649-657. Douglas...GCaMP6m/s in cells depdent on Cre, Flp, or Vcre Comprehensive Dual- and Triple-Feature Intersectional Single-Vector...;101(50):17404-9. Ca2+ indicators based on computationally redesigned calmodulin-peptide pairs. Chem Biol...sRGECO (constitutive or Cre- or Flp-dependent) Comprehensive Dual- and Triple-Feature Intersectional Single-Vector...locations. PLoS One. 2012;7(11):e49371. Direct comparison of a genetically encoded sensor and small molecule...NAD/NADH ratio changes in different cellular compartments. Biochim Biophys Acta. 2013 Nov 25. pii: S0304...

...most common is to use fluorescence microscopy to compare the localization of your protein of interest with...Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein Collection...table below to find markers of secretory pathway components. ID Plasmid Structure Targeting Gene/Signal Fluorescent...

...tetracycline on or rtTA is more appropriate. Basic components Components for a tetracycline off system are: Plasmid... your gene of interest tTA expression plasmid Components for a tetracycline on system are: Plasmid containing...can be made that continuously express a system component (e.g. tTA). Use of tetracycline or a derivative...toxicity, a known half-life (24 hours), and is compararably inexpensive. The induction profiles for other...

...parts can be combined to construct complex networks. This is accomplished through the use of assembly standards...suffix sequences that make it easy to combine components for synthetic biology research. Synthetic...meaning that the suffix will need to be reverse complimented for primer design. The restriction enzymes used...

...Activation Mediator (SAM) is an engineered protein complex for the transcriptional activation of endogenous...endogenous genes. It consists of three components: A nucleolytically inactive Cas9-VP64 fusion A sgRNA incorporating... : Expresses the MS2-P65-HSF1 activator helper complex with 2A GFP 61424 : sgRNA cloning backbone with...vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker 61427 : lentiviral...transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S*, Brigham MD*, Trevino AE, Joung J...

...containing 1 mM sodium azide. This buffer is not compatible for use in live cells and will interfere with...secondary antibody. The fluorescence pattern is compared to an untransfected control. Immunohistochemistry...secondary antibody. The tissue labeling pattern is compared to the mRNA expression pattern cited in the Tissue...HRP-conjugated secondary antibody. Protein expression is compared to an untransfected control and the protein size...

... mEGFP Fibrillarin Nucleolus (dense fibrillar component) 87428 MYH10-mEGFP AICSDP-24 mEGFP Non-muscle ...AICSDP-50 mEGFP Nucleophosmin Nucleolus (granular component) 109120 GJA1-mEGFP AICSDP-43 mEGFP Connexin-43...69 mTagRFP-T Nucleophosmin Nucleolus (granular component) 133963 UBTF-HaloTag AICSDP-80 HaloTag Nucleolar...mEGFP AICSDP-83 mEGFP EZH2 Polycomb repressive complex 2 164500 POLR2A-mEGFP AICSDP-117 mEGFP RPB1 RNA...

...Firefly luciferase. Plasmids are Gateway cloning compatible. Learn more about DULIP in our 27 Hot Plasmids...assess protein-protein interactions. NanoLuc® complementation : Protein-protein assays based on a split NanoLuc...more. nLuc/cLuc for split Firefly Luciferase complementation in plants. Bioluminescence Resonance Energy...Firefly luciferase fragment for split-luciferase complementation assay in plants using Gateway cloning. Use ...Fifrefly luciferase fragment for split-luciferase complementation assay in plants using Gateway cloning. Use ...

... Similar in principle to the ZF nuclease, the components of the array recognize individual nucleotides...Xanthomonas. The central TAL targeting domain is composed of 33-35 amino acid repeats. These repeats only...high. Since ZF targets are confined to sequences composed of triplets with corresponding zinc fingers, potential...TALEN kit. In this example, the final array is composed of 18 TAL effector repeats. The kit can be used...

.... With its rapid development (embryogenesis is complete in ~3 days), short generation time (~3 months)...dynamics in vivo. Bimolecular fluorescence complementation (BiFC) tools - Marco Morsch Lab. A vertebrate...Sauka-Spengler Lab. A genetic binary system for cell- and compartment-specific biotin labeling in zebrafish. pGCS Vector...GST and eGFP epitopes. Zebrafish Tol2 Gateway-compatible Kit for Neurodegenerative Disorders - Nicholas...

...EXOC3 EXOC4 EXOC5 EXOC6 EXOC7 EXOC8 Exocyst complex component FGFR FGFR1 FGFR2 FGFR3 FGFR4 Fibroblast growth... to find available plasmids for the gene. For components with multiple subunits or isoforms, individual...B1 RPTOR Regulatory associated protein of MTOR, complex 1 SAV1 Salvador family WW domain containing protein...