Addgene

We narrowed to 299 results for: pET

Showing: 181 - 200 of 299 results

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Addgene's Tips for Plasmid Quality Control

Type

Blog Post

...primers that bind within or must read through repetitive regions (such as LTRs or ITRs), hairpins, or ...
5 Steps to Writing Grant Proposals for Grad Students

Type

Blog Post

...assume that their advisor alone applies for these competitive grant applications; however, in some cases, your...
Adenoviral Vector Production and Troubleshooting

Type

Blog Post

...response against AdV. Importantly, recombination-competent adenovirus (RCA) can be generated if the E1 gene...
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

Type

Blog Post

...cloning strain DH5α. For plasmids with highly repetitive sequences (prone to recombination in bacteria...
Meet an Addgenie: Insights into Grad School, Career Paths, and More

Type

Blog Post

...discovered that science here is more stressful and competitive than back home. Scientists are often under a ...
Plasmids 101: Modular Cloning

Type

Blog Post

...Hurt for his help on this piece. Rob is postdoc in Peter Schultz’s group at Scripps Research, where he works...
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

Type

Blog Post

...Charlton J, Jung EJ, Mattei AL et al. (2020) TETs compete with DNMT3 activity in pluripotent cells at thousands...
A Look Back at One Year of Plasmid Sharing for COVID-19 Research

Type

Blog Post

... CR, Chu HY, Tortorici MA, Veesler D, Murphy M, Pettie D, King NP, Balazs AB, Bloom JD (2020) Protocol...
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

Type

Blog Post

...validation experiments performed will be useful in perpetuity. Second, plasmids are easy to store and share...
Hot Plasmids and Viral Preps - January 2021

Type

Blog Post

...cells. Casɸ from Biggiephage can eliminate competing mobile genetic elements. Image by Basem Al-Shayeb...
Viral Vectors 101: Chemogenetics

Type

Blog Post

... This blog post was originally written by Maya Peters Kostman in May 2020 and updated by Jason Nasse ...
A Look at Addgene's QC Process

Type

Blog Post

... platform, such as GC-rich regions and highly repetitive sequences and/or features. Most of these regions...
Cpf1 Update: Comparison to Cas9 and NgAgo

Type

Blog Post

... Up On Your Cpf1 Background Read About CRISPR Competitor NgAgo Learn How You Can Use piggyBac Vectors ...
Plasmids 101: Terminators and PolyA signals

Type

Blog Post

... weaker poly(A) signals like BGH. References Peters JM, Vangeloff AD, Landick R. Bacterial Transcription...
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

Type

Blog Post

...pathways are involved in RNA based surveillance of repetitive gene families and provide a way to guide inheritance...
Gaining Leadership Skills Volunteering at a Professional Organization

Type

Blog Post

...Postdoctoral Association (NPA) outlines 6 Core Competencies that all postdocs (but also PhDs) should develop...
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

Type

Blog Post

...attend conferences makes it very challenging to compete in today’s tough academic or industry job market...
Tips for a 1st time AAV user (by a Rookie AAV user)

Type

Blog Post

...bacterial strains, like NEB Stable. NEB Stable competent cells lack RecA, a protein that aids in the homologous...
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

Type

Blog Post

.... M., Pallaseni, A., Kuzmin, I., Raudvere, U., Peterson, H., Liberante, F. G., & Parts, L. (2023). Prediction...
PCR Applications for Copy Number Variation Assays

Type

Blog Post

... RQ value of 1.5 means you have either made a pipetting error or an incredible discovery. The range of...

Showing: 181 - 200 of 299 results

Results per page:

Addgene's Tips for Plasmid Quality Control

5 Steps to Writing Grant Proposals for Grad Students

Adenoviral Vector Production and Troubleshooting

Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

Meet an Addgenie: Insights into Grad School, Career Paths, and More

Plasmids 101: Modular Cloning

Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

A Look Back at One Year of Plasmid Sharing for COVID-19 Research

Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

Hot Plasmids and Viral Preps - January 2021

Viral Vectors 101: Chemogenetics

A Look at Addgene's QC Process

Cpf1 Update: Comparison to Cas9 and NgAgo

Plasmids 101: Terminators and PolyA signals

Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

Gaining Leadership Skills Volunteering at a Professional Organization

Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

Tips for a 1st time AAV user (by a Rookie AAV user)

PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

PCR Applications for Copy Number Variation Assays

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