We narrowed to 303 results for: pET

1. Summer SciComm: Preprints

   Type

   Blog Post

   ...//doi.org/10.1242/bio.059310 Fraser N, Mayr P, Peters I (2021) Motivations, concerns and selection biases...

2. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

   Type

   Blog Post

   ...used along other thiol-containing acceptors in competition assays. “I believe that improved biosensors or...

3. CRISPRainbow and Genome Visualization

   Type

   Blog Post

   ...(GAL4) was supplied. In addition to labeling repetitive telomeric sequences, Chen et al. successfully...

4. To Each HIS Own

   Type

   Blog Post

   ...then eluted using a high imidazole buffer that outcompetes the interaction between the protein of interest...

5. Addgene's Tips for Plasmid Quality Control

   Type

   Blog Post

   ...primers that bind within or must read through repetitive regions (such as LTRs or ITRs), hairpins, or ...

6. 5 Steps to Writing Grant Proposals for Grad Students

   Type

   Blog Post

   ...assume that their advisor alone applies for these competitive grant applications; however, in some cases, your...

7. Hot Plasmids and Viral Preps - January 2021

   Type

   Blog Post

   ...cells. Casɸ from Biggiephage can eliminate competing mobile genetic elements. Image by Basem Al-Shayeb...

8. Meet an Addgenie: Insights into Grad School, Career Paths, and More

   Type

   Blog Post

   ...discovered that science here is more stressful and competitive than back home. Scientists are often under a ...

9. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

   Type

   Blog Post

   ...Charlton J, Jung EJ, Mattei AL et al. (2020) TETs compete with DNMT3 activity in pluripotent cells at thousands...

10. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type

    Blog Post

    ... CR, Chu HY, Tortorici MA, Veesler D, Murphy M, Pettie D, King NP, Balazs AB, Bloom JD (2020) Protocol...

11. Adenoviral Vector Production and Troubleshooting

    Type

    Blog Post

    ...response against AdV. Importantly, recombination-competent adenovirus (RCA) can be generated if the E1 gene...

12. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    ...cloning strain DH5α. For plasmids with highly repetitive sequences (prone to recombination in bacteria...

13. Plasmids 101: Modular Cloning

    Type

    Blog Post

    ...Hurt for his help on this piece. Rob is postdoc in Peter Schultz’s group at Scripps Research, where he works...

14. Viral Vectors 101: Chemogenetics

    Type

    Blog Post

    ... This blog post was originally written by Maya Peters Kostman in May 2020 and updated by Jason Nasse ...

15. A Look at Addgene's QC Process

    Type

    Blog Post

    ... platform, such as GC-rich regions and highly repetitive sequences and/or features. Most of these regions...

16. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type

    Blog Post

    ...validation experiments performed will be useful in perpetuity.  Second, plasmids are easy to store and share...

17. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    ... Up On Your Cpf1 Background Read About CRISPR Competitor NgAgo Learn How You Can Use piggyBac Vectors ...

18. Plasmids 101: Terminators and PolyA signals

    Type

    Blog Post

    ... weaker poly(A) signals like BGH.  References Peters JM, Vangeloff AD, Landick R. Bacterial Transcription...

19. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type

    Blog Post

    ...pathways are involved in RNA based surveillance of repetitive gene families and provide a way to guide inheritance...

20. Gaining Leadership Skills Volunteering at a Professional Organization

    Type

    Blog Post

    ...Postdoctoral Association (NPA) outlines 6 Core Competencies that all postdocs (but also PhDs) should develop...