We narrowed to 304 results for: pET

1. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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   Blog Post

   ... and help each other practice meeting people – compete for fastest to set up a coffee date or most cards...

2. To Each HIS Own

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   ...then eluted using a high imidazole buffer that outcompetes the interaction between the protein of interest...

3. Summer SciComm: Preprints

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   Blog Post

   ...//doi.org/10.1242/bio.059310 Fraser N, Mayr P, Peters I (2021) Motivations, concerns and selection biases...

4. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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   Blog Post

   ...used along other thiol-containing acceptors in competition assays. “I believe that improved biosensors or...

5. CRISPRainbow and Genome Visualization

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   Blog Post

   ...(GAL4) was supplied. In addition to labeling repetitive telomeric sequences, Chen et al. successfully...

6. Addgene's Tips for Plasmid Quality Control

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   ...primers that bind within or must read through repetitive regions (such as LTRs or ITRs), hairpins, or ...

7. 5 Steps to Writing Grant Proposals for Grad Students

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   Blog Post

   ...assume that their advisor alone applies for these competitive grant applications; however, in some cases, your...

8. A Look at Addgene's QC Process

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   Blog Post

   ... platform, such as GC-rich regions and highly repetitive sequences and/or features. Most of these regions...

9. Adenoviral Vector Production and Troubleshooting

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   Blog Post

   ...response against AdV. Importantly, recombination-competent adenovirus (RCA) can be generated if the E1 gene...

10. Hot Plasmids and Viral Preps - January 2021

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    Blog Post

    ...cells. Casɸ from Biggiephage can eliminate competing mobile genetic elements. Image by Basem Al-Shayeb...

11. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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    Blog Post

    ...discovered that science here is more stressful and competitive than back home. Scientists are often under a ...

12. Viral Vectors 101: Chemogenetics

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    Blog Post

    ... This blog post was originally written by Maya Peters Kostman in May 2020 and updated by Jason Nasse ...

13. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    Blog Post

    ...Charlton J, Jung EJ, Mattei AL et al. (2020) TETs compete with DNMT3 activity in pluripotent cells at thousands...

14. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    Blog Post

    ... CR, Chu HY, Tortorici MA, Veesler D, Murphy M, Pettie D, King NP, Balazs AB, Bloom JD (2020) Protocol...

15. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    Blog Post

    ...validation experiments performed will be useful in perpetuity.  Second, plasmids are easy to store and share...

16. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    Blog Post

    ...cloning strain DH5α. For plasmids with highly repetitive sequences (prone to recombination in bacteria...

17. Plasmids 101: Modular Cloning

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    Blog Post

    ...Hurt for his help on this piece. Rob is postdoc in Peter Schultz’s group at Scripps Research, where he works...

18. Cpf1 Update: Comparison to Cas9 and NgAgo

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    Blog Post

    ... Up On Your Cpf1 Background Read About CRISPR Competitor NgAgo Learn How You Can Use piggyBac Vectors ...

19. Plasmids 101: Terminators and PolyA signals

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    Blog Post

    ... weaker poly(A) signals like BGH.  References Peters JM, Vangeloff AD, Landick R. Bacterial Transcription...

20. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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    Blog Post

    ...pathways are involved in RNA based surveillance of repetitive gene families and provide a way to guide inheritance...