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We narrowed to 562 results for: pac

Showing: 181 - 200 of 562 results

  1. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    ...this time more visible and thereby have a positive impact on science. ReFigure saves time, knowledge and ...definitely give you credit for it and increase your impact! Download ReFigure extension and visit ReFigure.org... with Joe Akin. She envisions a world where the impact of teaching and doing science is rapid, concrete...

  2. Advice for Choosing a Research Project

    Type
    Blog Post
    ...the focus shifts to choosing an unexplored and impactful research topic that aligns with your interests...when selecting a research project. What will the impact be in a best case scenario? If you get your dream...what will your paper look like? What will be the impact on your field? You want to choose a research question...

  3. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    ...around the world were closed and not receiving packages. So the entire team mobilized to figure out how...subsequent fluctuations in distribution. I don’t have space in this blog to call out every Addgene Team because...workforce for when we need to be working at full capacity to serve the scientific community. First, we applied...

  4. What the HEK?

    Type
    Blog Post
    ... here.   Virus production HEKs are the go-to ‘packaging cells’ for viral production because they express...need only transfect the ‘helper’ plasmids (viral packaging machinery) along with the viral plasmid containing...the cargo of interest, and HEKs will produce and package viral particles. The viral particles are then filtered...

  5. TALEN Plasmids and Kits

    Type
    Collection
    ...Construction and Evaluation Accessory Pack Takashi Yamamoto This accessory pack contains modified pFUS array vectors...

  6. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ... SARS-CoV-2 are the lentiviral production and packaging vectors VSV-G and psPAX2, which were used in a...Repositories around the world facilitate the rapid pace of SARS-CoV-2 research by removing logistic hurdles...and financial support to maintain repositories’ capacity for storing, validating, and distributing such...

  7. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...the human interactors we found in our map have an impact on virus replication. While we and our collaborators...one are in a pLVX vector background, so you can package them into lentiviral vectors if you need to. Additionally...get notified!) Attacking coronavirus, one FedEx package at a time Since the publication of our paper, our...

  8. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...keep viruses frozen during shipment was having an impact on the samples. We therefore devised a few experiments...solution thawed, as the CO2 was confined to the tube headspace when the samples were frozen. This was clear by...tubes prior to thaw released the CO2 from the headspace, thus mitigating the pH effects of dry ice storage...

  9. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...individually overexpressed and available for lentiviral packaging and delivery to target cells as a pooled library... 2023). Fig. 1: Barcoded TF library was packaged in lentiviral particles and used to transduce ...so it is an invaluable tool to be used in this capacity. Similar to the Human TFome Library, the MORF ...

  10. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...Biology and, on occasion, would meet in the same space where BosLab exists today. Back then, it consisted...Kendall square, but we saw the potential in the space and decided to reactivate it. We started out by ...projects at BosLab. Some are entrepreneurs using our space to perform early-stage experiments. For example,...

  11. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...Archives allots for 25 MB of space per user, but, if you need more space, you can sign up for a plan. ... by iGreenEarth AMINO is a very simple app that packs important biological information, amino acid abbreviations...

  12. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ... applied, but these methods can have a negative impact on cellular health. Another limitation of traditional... them capable of diffusing in the extracellular space; they also have low immunogenicity and cellular ...less likely to be sequestered in the extracellular space. In addition, rAAV2-retro’s altered amino acid sequence...diffuse long distances within the extracellular space along an axon, this does not seem to be the case...

  13. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...Usually Destroyed Yes, Cpf1 cleaves 5' of the protospacer Multiplexing CRISPR-Cas9 options before Cpf1...which are often more difficult to transfect or package into viral vectors. Cas9 multiplexing vectors are...bind and cleave the lentiviral RNA, preventing packaging of the virus.  Reversing the orientation of the...

  14. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...Discrepancies in transduction efficiency can have a major impact on experimental design and interpretation of results...transduced by the vector. One method for minimizing the impact of this phenomenon is to use a retrograde recombinase-expressing...cell bodies of the projection neurons reside.   Impact for scientists While the mechanisms by which retrograde...

  15. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ... by spaces. File names with more than one word should be named in the camelCaseFormat since spaces between...the basics. However, these notes take up a lot of space and don’t tell you why you did something or what...

  16. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ... resulting in variable gene expression that can impact observed phenotypes. Factors such as the growth...medium, growth temperature, and growth rate can all impact plasmid copy number in a cell. One can attempt... from a plasmid to the genome without greatly impacting the level of gene expression. For example, the...

  17. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...at some point, decide early on what to do in a namespace collision. It may even be worth it to have extra... you're solving, but consistency is key. Some namespace collisions you may encounter: vector, sequence...Use tried-and-true tools We use Python, Django, Apache, and MariaDB. Using tried-and-true tools means ...

  18. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...repeats separated by an 8-bp long asymmetric core spacer sequence. The asymmetry in the core sequence gives... double-strand DNA break occurs within the core spacer of each loxP site and the two strands are ligated...been created, which contain a unique asymmetric spacer "NNNTANNN", where "N" indicates which bases may...

  19. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ... and impact the field - its encouraged to dream big! It is crucial that the goal and the impact of the...research plan Most grant applications restrict the space available to describe the proposed work. Thus, every...

  20. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    .... https://pubmed.ncbi.nlm.nih.gov/24390935/ Hypercompact genome editor CRISPR-Casɸ By Melina Fan The ...toolbox with their discovery of CRISPR-Casɸ, a hypercompact genome editor from the Biggiephage clade of ...the Doudna Lab. Scientists looking for more compact genome editors due to delivery constraints and ...
Showing: 181 - 200 of 562 results