We narrowed to 406 results for: pho

1. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

   Type

   Blog Post

   ... to students, like how to pour a gel [for] electrophoresis or doing a dissection video. I did that in ...themselves. “You open up the box and there’s a gel electrophoresis tray in there. Or the dissection specimens....

2. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

   Type

   Blog Post

   ...the overlap between groups.  They found that photostimulating the Kolmer-Agduhr cells was sufficient to ...Kolmer-Agduhr cells on one side of the body required photostimulation in order to drive the full symmetrical response...

3. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

   Type

   Blog Post

   ...affordable biosensors that detect herbicides, such as glyphosate, in water. He aims to leverage a glycine oxidase...oxidase reaction to indirectly detect glyphosate via H2O2, one of the reaction’s metabolites. One useful...

4. Understanding GPCR Signaling Using TRUPATH

   Type

   Blog Post

   ...FRET), in which a donor fluorophore is used to excite an acceptor fluorophore. The difference for BRET...

5. An Addgene Summer Internship

   Type

   Blog Post

   ...and undigested samples of the clones on an electrophoresis gel in order to differentiate which clone had...Once we had gotten our results back from the electrophoresis, my manager and I were unsure if there were...

6. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

   Type

   Blog Post

   ...element (tyrosine sulfation consensus sequence, fluorophore, or APEX2), allowing for quantitative analysis...fused with GFP, YFP, mCerulean, or other related fluorophores. Find all of the nanobody toolkit plasmids here...

7. RUBY-Red Siliques

   Type

   Blog Post

   ... Figure 1: An adult plant expressing RUBY. Photos by Andrew Willoughby   Using RUBY as a selection...green tissues is slightly darker or slightly brown. Photos by Andrew Willoughby The ease of observing ...

8. Antibodies 101: Chimeric Antibodies

   Type

   Blog Post

   ...use a red fluorophore-tagged anti-rat IgG1 secondary to detect Protein A and green fluorophore-tagged anti-mouse...

9. New and Upcoming Viral Vectors - September 2019

   Type

   Blog Post

   ...scientists access to a wide array of promoters and fluorophores for validating and testing purposes. We will...specificity of influence measurements by reducing photostimulation of the axons and dendrites of non-targeted...

10. Plasmids 101: Gram Negative and Positive Bacteria

    Type

    Blog Post

    ...Image credit: Mark Perkins https://www.flickr.com/photos/occbio/7092385559 For more specific procedural...in marine biology and minoring in studio arts photography.  References and resources References Silhavy...

11. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type

    Blog Post

    ...these TFs to see if the differentiated cells had morphologies consistent with the predicted cell type (e.g...neuron predicted differentiation), and 15/17 morphologically aligned. Documentation of the network that ...

12. Plasmids 101: Inducible Promoters

    Type

    Blog Post

    ...sensing protein YFI. When light is absent, YFI phosphorylates FixJ, which binds to the FixK2 promoter to ...

13. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type

    Blog Post

    ...designed by the Toettcher Lab, OptoNBs are photoswitchable engineered nanobodies whose binding of their...cascade. This signaling is a lot like a game of telephone, until the message reaches the nucleus where gene...

14. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type

    Blog Post

    ...transmission within minutes. Constructs using the photodimerizer system CRY2-CIB1 to regulate botulinum neurotoxin...gas-filled protein structure that water-bound photosynthetic organisms use for buoyancy. These proteins ...

15. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type

    Blog Post

    ...tumorigenesis assays, drug epistasis assays, and morphological profiling can be used as generalizable strategies... are described in this paper Systematic morphological profiling of human gene and allele function ...

16. Multiplex Genome Editing with CRISPR-Cpf1

    Type

    Blog Post

    ...example of how this works. Make sure to order 5’ phosphorylated oligos or treat with T4 PNK. Delivery options...

17. Sweating the Small Stuff: Details in the Lab

    Type

    Blog Post

    ..., a large set of headphones can act as a Do Not Disturb sign, even if the headphones aren’t actually playing...

18. Antibodies 101: Flow Cytometry Controls

    Type

    Blog Post

    ...should have a single color control for every fluorophore you intend to use in your panel. Using single...have the same Ig subclass, and be in the same fluorophore (e.g., if you are staining for anti-mouse Ly6C...

19. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type

    Blog Post

    ...inactivation maximum of 590 nm light. Aurora and Phobos optogenetic tools - A collection of bi-stable anion... medicine, evolution of body plans, synthetic morphology, and even artificial intelligence," says Michael...

20. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    ...selecting a bright fluorescent protein and a photostable fluorescent protein. In the third post of this...verify a number of key properties (brightness, photostability, oligomerization) of a number of promising ...