...)/~500 (off) PhoCl Photocleavable protein engineered from a green-to-red photoconvertible fluorescent ...enhancements include: Increased photocurrent amplitude Examples: iChloC, SwiChRca, Phobos, Aurora Browse Channelrhodospin...reduces photocurrent amplitude 490 ChR/T159C T159C mutation; displays increased photocurrents 470 SFO/...bacterial photoreceptors to create protein systems controlled by light. These “photoswitchable” proteins...Kv) channels fused to LOV2 photoswitch. Illumination causes the photoswitch to unfold, lowering the toxin's...synthetic, photosensitive kinase, which uses the ubiquitous flavin mononucleotide as its chromophore, and phosphorylates... 400 UVR8 domains UVR8 plant photoreceptor protein that forms photolabile homodimers with slow reversal...

... curve with known amounts of protein. Fluorophores Fluorophores are light-sensitive molecules which emit...IF uses fluorophore color to differentiate between the targets of interest, if your fluorophore is bound...the fluorophores used is important. It is best to inversely pair the brightness of the fluorophore with...If a bright fluorophore is on an abundant target, it obscures fainter signals. Fluorophores can emit signals...storing your antibody: Antibodies conjugated to fluorophores should never be stored below 4 °C. Certain isotype...molecules are horseradish peroxidase (HRP) and fluorophores. Horseradish peroxidase (HRP) HRP performs a...specific wavelength. While more expensive than HRP, fluorophores are available in a range of colors activated...

...envelope, Phoenix-ECO, (for infection of mouse and rat cells), or an amphotropic envelope, Phoenix-AMPHO (for...of the viral packaging cell line. For example, Phoenix, a second generation γ-retrovirus packaging cell... produced in just a few days. Another variant, Phoenix-gp, contains only Gag-Pol and enables additional...

...activate neuronal firing through stimulating phospholipase C, which releases intracellular calcium stores...molecule driven by FRET-modified bioluminescence. Neurophotonics. 11:021005. PMID: 38450294 (Link opens in a...luminopsins with improved coupling efficiencies. Neurophotonics. 11:024208. PMID: 38559366 (Link opens in a...

...SpCas9-HF1 - disrupt Cas9’s interactions with DNA phosphate backbone HypaCas9 - increase Cas9 proofreading...fusing the anti-CRISPR protein AcrIIA4 with the photosensitive LOV2 domain . Several labs have also controlled...: Epigenetics Visualizing Genomic Loci Using Fluorophores CRISPR imaging has numerous advantages, including...11: Overview of visualizing genomic loci with fluorophores using CRISPR Browse Plasmids: Visualize Purify...Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho, E., Xu, H., Sasakawa, N., Naito, Y., Nakada, S...

...of restriction enzymes, DNA ligase, and gel electrophoresis allowed for the ability to move specific fragments...Delta(lacZYA-argF) U169 recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 tonA JM109 Addgene; Promega...4 standard bases are tagged with a different fluorophore so they can be distinguished from one another...

...promoter PGK Constitutive Mammalian promoter from phospholycerate kinase gene TRE Inducible Tetracycline response...

...gRNA Presence of a selectable marker (drug or fluorophore) Delivery method Expression System Components...identify DNA fragments. PCR amplification and gel electrophoresis (for HDR or NHEJ): For large deletions or insertions...

...plasmid cloning, and virus, including: Gel Electrophoresis Bacterial Transformation Plasmid Cloning Creating...

...localization; an introduction to non-protein fluorophores; and much more! Close About Overlay Select Viral...

... AAV4, AAV5, AAV6, AAV9 Pancreas AAV6, AAV8 Photoreceptor Cells AAV2, AAV5, AAV8 RPE (Retinal Pigment ...