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We narrowed to 416 results for: plasmids 101

Showing: 181 - 200 of 416 results

  1. CRISPR Guide Update

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    Blog Post
    ... helps scientists understand how to use CRISPR plasmids in the lab and the vast range of possibilities...resources on the Addgene blog PRIME Editing CRISPR 101: Cas9 vs the Other Cas(s) Progress Towards a PAM-...

  2. Isolating Genomic Regions of Interest with the CRISPR System

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    Blog Post
    ... tag expressed on a plasmid, allowing for in vivo biotinylation through a plasmid-based biotin ligase....the Addgene blog Download the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...which can easily be cloned into a gRNA expression plasmid. Experimental use Since the initial report describing...currently no cure (Campbell et al., 2018). Find plasmids from the Fujii lab! Conclusions CRISPR-mediated... PubMed PMID: 24201379. PubMed Central PMCID: PMC3821016. Fujita, Toshitsugu, et al. "Identification of...

  3. Addgene's Top Blog Posts from 2020

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    Blog Post
    ...Here we cover five methods to do this. 5. Plasmids 101: Plasmid Incompatibility by Leah Schiesow If you're...service features 210 plasmids: empty backbones, fluorescent protein tags, SARS-CoV-2 plasmids, etc. It is our...to Elute and Store Your Plasmid DNA? by Paolo Colombi Should I store my plasmid in TE, Tris, or water?... We've had 11 blog posts on COVID-19 featuring plasmid tools from depositors who quickly shared their ...you're thinking about hosting multiple plasmids in one cell, it might be that you can't stably maintain...This blog post features two mechanisms that cause plasmids to be incompatible. 4. RNA Extraction Without...CRISPR to detect SARS-CoV-2, including many with plasmids deposited at Addgene! 2. DNA Purification Without...

  4. CRISPR Cheat Sheet

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    Blog Post
    ...you a brand new third edition of Addgene’s CRISPR 101 eBook. This new edition of the eBook includes the...commonly used CRISPR nuclease. Note: Most CRISPR plasmids from Addgene produce either a gRNA, a nuclease... to deliver. Not all cells efficiently take up plasmids used to produce CRISPR systems    ...

  6. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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    Blog Post
    ...resources on the Addgene blog Plasmid 101 Protein Expression Plasmid 101 Biotinylation Other resources...vivo studies (Che and Khavari, 2017). Find APEX plasmids at Addgene! Since the development of BioID and... specific locations in the cell.   Find BioID2 plasmids here APEX2: A more sensitive ascorbate peroxidase... They named this mutant APEX2.  Find the APEX2 plasmids! TurboID and minturbo: Rapid and non-toxic proximity...allowing for more precise temporal control.  Find plasmids for TurboID and miniturbo Split-BioID: A conditional...streptavidin and separated via flow cytometry.  Find plasmids for EXCELL here! Since BioID was developed in ... PubMed PMID: 24114984. PubMed Central PMCID: PMC3910158. Ramanathan, Muthukumar, et al. "RNA–protein ...

  7. Five Popular Model Organisms, Part 2

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    Blog Post
    ... Organisms blog post Read more about plasmids in our Plasmids 101 blog series Learn more about CRISPR ...box” for its common use in plasmid manipulation and genetics research. Plasmids are ideal tools for studying...editing in our CRISPR 101 blog series Resources on Addgene.org: Browse our plasmid collection page Find...Find more plasmids and resources for plant resources on Plant Plasmids Collection Page Read our molecular...disease models.  Check out Addgene’s collection of plasmids containing Rattus norvegicus genes! Mexican axolotl...several vectors for use in the axolotl including plasmids for use in CRISPR and Tol2 transposase induced... and after embryogenesis. Addgene has over 600 plasmids that contain Xenopus genes and a tool-kit from...

  8. To Codon Optimize or Not: That is the Question

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    Blog Post
    ...usage bias in Plasmids 101: Codon usage bias Read more about plasmids in our Plasmids 101 blog series Learn...Reference Read about genetic code expansion Browse our plasmid collection page  ... in Biotechnology 22:346–353 . https://doi.org/10.1016/j.tibtech.2004.04.006 Hausser J, Syed AP, Bilen.... Genome Research 23:604–615 . https://doi.org/10.1101/gr.139758.112 Mauro VP, Chappell SA (2014) A critical...Molecular Medicine 20:604–613  https://doi.org/10.1016/j.molmed.2014.09.003 Nakamura Y (2000) Codon usage...Neuroscience Methods 124:75–81 . https://doi.org/10.1016/s0165-0270(02)00362-x Stadler M, Fire A (2011) ...USA 107:3645–3650 https://doi.org/10.1073/pnas.0909910107 Varani G, McClain WH (2000) The G·U wobble base...

  9. What's the Best Way to Elute and Store Your Plasmid DNA?

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    Blog Post
    ... kit Read our blog posts about plasmid cloning Browse our Plasmids 101 blog posts Resources on Addgene.org...fragmenting plasmid DNA, PCR product, or chromosome. One of the main causes of degradation during plasmid DNA ...Biology Reference Find protocols on plasmid cloning Find plasmids for your research ...Nowadays, the most common E. coli strains used for plasmid amplification are endA negative, meaning the gene...degradation and which experiments you can use your plasmid.  DNA elution choices: TE, Tris buffer, or water...polymerases. In the situation where you need to use plasmid in TE for any enzymatic reaction, an effective ...choices that companies offer for the elution of plasmid DNA through their kit. The advantage of using a...

  10. Of Myc and Men

    Type
    Blog Post
    ...Addgene blog Antibodies 101: Monoclonal Antibodies Plasmids 101: Peptide Tagging Plasmid-based Recombinant ... recombinant anti-c-Myc [9E10] at Addgene Find plasmids with the myc-tag  ...binding protein. Cell 46:291–300. https://doi.org/10.1016/0092-8674(86)90746-4 Munro S, Pelham HRB (1987)... ER proteins. Cell 48:899–907. https://doi.org/10.1016/0092-8674(87)90086-9  Schüchner S, Behm C, Mudrak...

  11. Five Popular Model Organisms

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    Blog Post
    ...proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org Browse our plasmid collection... resistant to chemotherapy. You can find these plasmids at Addgene. Another advantage of mice as model... pancreas. They first tagged these cells using plasmids. Then using HOTcre, a method that uses heat induction... feeding the worm bacteria expressing specific plasmids (L4440) that contain your gene of interest (Fire...calcium signaling in the entire adult brain, using a plasmid that expresses a genetically encoded calcium sensor...al., 2015). If you are interested in C. elegans plasmids and resources check out Addgene’s Worm Expression...pages Read our molecular biology reference Find plasmids for your research ...

  12. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

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    Blog Post
    ...Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection.  mEmerald plasmids and many more...Molecular Expressions collection including some 3,300 plasmids along with image galleries and educational resources...resources to go with them. This collection of plasmids is available in an easily searchable format on Addgene...Fluorescent Protein Collection webpage. These plasmids are divided into the following categories: Over...list of cell markers all tagged with mEmerald. Plasmids to monitor protein-protein interactions through...Specialty constructs for biosensing. Over 300 plasmid backbones spanning a rainbow of fluorescent proteins...proposition, explained Michelle Baird, who has created plasmids as a technician in the Davidson lab for the last...

  13. pSiM24: Simplifying Plant Genetic Engineering

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    Blog Post
    ...GreenGate Plasmids Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More Plasmids 101: Origin...stability of modified Ti plasmids during plant infection, as well as plasmid yield during preparation ...Agrobacterium carries a “tumor-inducing” or Ti plasmid, which allows it to transfer genetic material into...in two species: Agrobacterium and E. coli. Like plasmids used in other systems, binary vectors are cloned...It’s a lot of information to fit into a single plasmid, so most first-generation binary vectors are quite...pSiM24 are suitable for many applications, the plasmid can also be customized due to its modular structure...Addgene Check out Addgene’s plant vectors! Find the plasmids from this paper at Addgene. Other relevant blog...

  14. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...Antibodies 101: Introduction to Immunofluorescence Antibodies 101: Epitope Availability Antibodies 101: Introduction...Addgene.org Find recombinant antibodies at Addgene Find plasmids encoding recombinant antibodies at Addgene   ...

  15. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

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    Blog Post
    ...the Addgene blog Plasmids 101: Gene, enhancer, and promoter trapping Plasmids 101: The Promoter Region...has culminated in a collection of AAV enhancer plasmids with high specificity and brightness, now available...GTA contains data on thousands of AAV enhancer plasmids, which are all available at Addgene. This allows...including the Allen Institute for Cell Science Plasmid Collection and the BRAIN Armamentarium Collection...Region – Let's Go! Plasmids for Endogenous Gene Tagging in Human Cells Resources on addgene.org Allen...Armamentarium Collection Allen Institute for Cell Science Plasmid Collection ... to cortical cell types. Cell. https://doi.org/10.1016/j.cell.2025.05.002 Hunker, A. C., Wirthlin, M. ...

  16. Plasmid Cloning by PCR

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    Blog Post
    ... on All Things Plasmid with Our Plasmids 101 Series Get Tips on Verifying Your Plasmid Resources on Addgene.org...Check out Our Plasmid Reference Pages Deposit Your Completed Plasmids Find the Perfect Plasmid for Your Research...recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid Bonus...recipient plasmid. Remember to insert your DNA in the correct orientation in the recipient plasmid by viewing... of DNA so that it can be easily cloned into a plasmid of interest. You can use similar processes to add... Interest (YGOI) for ligation into a recipient plasmid. Designing primers for PCR based cloning   ...this example). Assuming you are amplifying from plasmid DNA (rather than from genomic DNA or a cDNA library...

  18. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    ...because it relies on a single BiFC pair. Find BiFC plasmids for your experiment at Addgene! How to Design ...proteins, check out our article: Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection...straightforward in easy-to-transfect cell lines where plasmid dosage can be tightly controlled. This is also ...the Addgene blog Browse our Fluorescent Protein 101 blog posts Read our blog post covering an introduction...Resources on Addgene.org Find fluorescent protein plasmids and resources Visit our Molecular Biology Reference...Complementation. Molecular Cell 9:789–798. https://doi.org/10.1016/s1097-2765(02)00496-3 Kerppola TK (2006) Design...Harb Protoc 2013:pdb.prot076497. https://doi.org/10.1101/pdb.prot076497 Kim Y, Jung JP, Pack C-G, Huh W-...

  19. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...resources on the blog CRISPR 101: Cas9 vs. The Other Cas(s) CRISPR 101: Anti-CRISPR Proteins Switch Off...its partner in crime, Cascade. Find Cascade-Cas3 plasmids in Addgene's collection! A “complex” system Cas3... Various labs have designed all-in-one Cascade plasmids — such as the Tomoji Mashimo lab and the Tilmann...resources on Addgene.org CRISPR guide Cascade-Cas3 Plasmid collection ...Molecular Cell, 70(3), 385-394.e3. https://doi.org/10.1016/j.molcel.2018.03.031 Xiao, Y., Luo, M., Dolan, ...and Genome Editing, 3–4, 100013. https://doi.org/10.1016/j.ggedit.2022.100013 O’Brien, R. E., Bravo, J. ...Molecular Cell, 83(5), 746-758.e5. https://doi.org/10.1016/j.molcel.2023.01.024 Morisaka, H., Yoshimi, K.,...

  20. New and Upcoming Viral Vectors - December 2019

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    Blog Post
    ...constructs from Christopher Harvey’s lab. These plasmids localize opsins to the soma to avoid off target...neighboring neurons. We have also packaged hChR2(H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn...H134R)-EYFP that is both Cre and Flp dependent. Plasmid Serotype Name 124603 AAV9 pAAV-EF1a-DIO-ChrimsonR-mRuby2... has now expanded to offer many of our control plasmids in new serotypes. See our Serotype Testing AAV...AAV inventory for the entire collection! Plasmid Serotype Name 51502 AAV5 pCAG-FLEX-EGFP-WPRE 114471... jGCaMP7 from Janelia, including three jGCaMP7 plasmids now packaged in AAVrg. jGCaMP7s, the variant with...post Overview of AAV Encoded Calcium Sensors). Plasmid Serotype Name 104489 AAVrg pGP-AAV-syn-jGCaMP7b-WPRE...
Showing: 181 - 200 of 416 results