...select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used together ...first CRISPR experiment. CRISPR 101 eBook : Based on our popular CRISPR 101 blog series, we've organized ...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR... CRISPR Plasmids and Resources... use CRISPR technology in your own lab. Browse plasmids below or check out our CRISPR resources on how...inhibition, and barcoding experiments. Browse CRISPR Plasmids by Function Genome Engineering Cut Wild-type Cas...experiment based on a variety of factors. gRNAs gRNA plasmids to target specific genes. Other Applications Purify...

...AAV) Plasmids Additional Resources: AAV Guide Biosafety Guide All Viral Plasmids Blog: Plasmids 101-Viral...AAV use in CRISPR Viral Vectors 101 eBook Packaging Plasmids AAV Plasmids Adeno-associated viruses (AAV)...with the plasmid that encodes the viral genome (typically called cis -plasmid or transfer plasmid). Helper...Helper Plasmid ID Plasmid Serotype Description PI 112867 pAdDeltaF6 All Helper plasmid for AAV packaging... Viral Plasmids Adeno-associated Virus (AAV) Plasmids Adeno-associated Virus...service, and see which plasmids are available as viral preparations. AAV Packaging Plasmids The following tables...particles, a helper plasmid (encoding adenovirus E4, E2A and VA) and a RepCap plasmid (encoding AAV Rep ...

...Plasmids 101-Viral Vector Elements Viral Vectors 101 eBook Overview of Viral Plasmids Which Type of Virus Should...for select plasmids are now available through Addgene, learn more here . Browse Viral Plasmids by Type Visit... to find plasmids that can be used to engineer various types of viruses. Lentivirus Plasmids | Guide Lentiviruses...transgene expression. Overview of Viral Plasmids While plasmids are typically used to encode, propagate...organism. Figure 1. Using viral plasmids to generate virus. A) Viral plasmids have been engineered such that...genomes have been adapted into a plasmid-based technology, such that plasmids can be used to create viruses...researchers can introduce plasmids to a host to generate virus. Furthermore, these plasmids can be modified to...

...Reporter Plasmids You may also like… Plasmids 101 Addgene's Molecular Biology Guides Plasmids 101: E. coli...interest. Plasmid ID Promoter Inducer(s) PI Purpose Murray Lab pBEST plasmids Various Plasmids pBAD, OR2...Purification Controlled Expression Reporter Plasmids Reporter Plasmids Tagging and Visualization Purification...coli strains for protein expression Plasmids 101: Common Lab E. coli Strains Why Bacteria? E. coli are...list of our bacterial CRISPR plasmids, see here . pGRG Series Various Plasmids Tn7 Transposition Nancy Craig...green light. BIOFAB plasmid set 1000000037 N/A N/A Drew Endy This kit contains plasmids with various combinations...specific level. Return to Top Reporter Plasmids Reporter plasmids can be used to detect events and molecules...

...Accessory Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...particles See Addgene's Viral Plasmids and Resources page for more information. Plasmids Expressing Spike and ...Addgene plasmid #141347) is available in standard format and as a Lentiviral prep . ID Plasmid Description...COVID-19 and Coronavirus Plasmids & Resources Ginkgo Bioworks COVID-19 Plasmid Collection Return to top...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's collection of plasmids for viral pseudotyping with SARS-CoV-2 spike protein. Collections...

... Viral Plasmids Adenovirus Plasmids Adenovirus Plasmids Additional Resources: Adenovirus...Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are a class ...yeast. Two plasmids, termed a shuttle plasmid and an adenoviral (also called backbone) plasmid, are recombined...contains AdEasy-1™ plasmid Vogelstein Adenoviral Plasmids Common shuttle and adenoviral plasmids. Want an adenoviral... Venus tag; See plasmid 62622 for plasmid without Venus Wang Adenoviral Kits Plasmids containing modular...Strain for recombination between shuttle plasmid and adenoviral plasmid Vogelstein 16399 AdEasier-1 BJ5183 ...adenoviral plasmid with your gene of interest? Search our database for adenoviral transfer plasmids that have...

... Viral Plasmids γ-Retroviral Plasmids γ-Retroviral Plasmids Additional Resources...Guide Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus... and Packaging Plasmids Common γ-retroviral envelope and packaging plasmids. ID Plasmid Type Description...with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing envelope plasmid Trono 1733 pCI-VSVG... envelope plasmid Kasparov 35617 pCAG-Eco Envelope Ecotropic MLV expressing envelope plasmid Nienhuis ...VSV-G-expressing envelope plasmid Nienhuis and Salmon Popular γ-Retroviral Transfer Plasmids Common γ-retroviral...γ-retroviral transfer plasmids. Want a retroviral plasmid with your gene of interest? Search our database for ...

...Collections INTRSECT Plasmid Collection INTRSECT Plasmid Collection INTRSECT Implementation Plasmids References ...novel INTSRECT plasmids for groups interested in producing their own INTRSECT plasmids. Online spreadsheet...Find intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and...Flp-recombinase-expressing transgenic mouse and rat lines. Plasmids In addition to EYFP and ChR2-EYFP, a large number...rabies targeting genes. Recombinases Addgene ID Plasmid Logic Sites and Mutations 55636 pAAV-EF1a-Cre None...pAAV-CaMKIIa-Flpo None Single recombinase-dependent Addgene ID Plasmid Logic Sites and Mutations 55641 pAAV-Ef1a-fDIO ...recombinase-dependent: Fluorophores Addgene ID Plasmid Logic Sites and Mutations 55650 pAAV-hSyn Con/Fon...

...delivery of a plasmid. Below, browse our selection of empty vectors for cloning in shRNAs. Plasmids for constitutive...Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral... Plasmid Collections Mammalian RNAi Mammalian shRNA Tools... libraries Lentiviral packaging and expression plasmids Background RNA interference (RNAi) is an RNA-mediated...inducible (Tet) expression are available. To find plasmids containing RNAi components, like Argonaute, please...Lentiviral vectors for constitutive shRNA expression ID Plasmid Description PI 10878 pLKO.1 ‐ TRC cloning vector... for conditional (Cre-lox) shRNA expression ID Plasmid Description PI 11578 pSico Cre addition causes ...

...in a 1-plasmid system, in which Cas9 is included on the gRNA-containing plasmid, or a 2-plasmid system...editing CRISPR 101: Homology directed repair CRISPR 101: Validating your genomic edit CRISPR 101: Multiplex...several gRNAs from the same plasmid ensures that each cell containing the plasmid will express all of the ...generate stable cell lines (for details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression...Additionally, many plasmids containing validated gRNAs are now available through Addgene. These plasmids contain ...Blog References Website CRISPR plasmids and resources at Addgene CRISPR 101 eBook (2nd edition) CRISPR software...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science...

...human cancers. Ras Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's collection of plasmids for the Ras pathway. Ras is a small GTPase and constitutively active...mTOR Pathway p53 Pathway Background Ras Pathway Plasmids Ras Gene List Resources Background Ras is a small... - Gene List Click on a name to find available plasmids for the gene. For components with multiple subunits...World J Gastrointest Oncol. 2013 May 15; 5(5): 97–101. doi: 10.4251/wjgo.v5.i5.97. PubMed PMID: 23799159...

...repository of published capsid use . Viral Vectors 101: Systemic Capsids PHP.eB These viral vector preparations...preparations were produced with the pUCmini-iCAP-PHP.eB plasmid (Addgene #103005) . Note on PHP.eB tropism and ...preparations were produced with the pUCmini-iCAP-PHP.S plasmid (Addgene #103006) . Browse Available PHP.S AAV ...preparations were produced with the pUCmini-iCAP-PHP.V1 plasmid (Addgene #127847) . Browse Available PHP.V1 AAV...were produced with the pUCmini-iCAP-AAV.MaCPNS1 plasmid (Addgene #185136) . Browse Available MaCPNS1 AAV...were produced with the pUCmini-iCAP-AAV.MaCPNS2 plasmid (Addgene #185137) . Browse Available MaCPNS2 AAV...were produced with the pUCmini-iCAP-AAV.CAP-B10 plasmid (Addgene #175004) . Browse Available CAP-B10 AAV...

...and Myers Tagging Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor... as the plasmids and protocols needed for using them, are described below. Read our CRISPR 101 post for.... Multiple gRNA plasmids may be used. The HDR (homology directed repair) donor plasmid with homology arms...individual tagging event. The kit contains 26 plasmids; 21 of the plasmids are for use in SapTrap reactions for...the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR plasmids for endogenous tagging of your gene of interest. CRISPR...

... Plasmid Collections Chemogenetics Plasmids Chemogenetics Plasmid Collection You may...Chemogenetic Plasmids This table contains Addgene's collection of chemogenetic plasmids plasmids. The table...receptor type, plasmid type, promoter, reporter or fusion protein, and whether the plasmid is Cre- or Flp...found. Browse all DREADD plasmids Do you have suggestions for other plasmids that should be added to this...Chemogenetics AAV Blog: Chemogenetics Viral Vectors 101 Blog: Which option should I choose? Chemogenetics...Chemogenetics vs. Optogenetics Background Chemogenetics plasmids encode genetically engineered receptors that are...ready-to-use AAV preparations of select chemogenetic plasmids; these are noted with the viral service icon ....

...Collections Luciferase Plasmids Luciferase Plasmid Guide You may also like... Fluorescent Proteins Blog: Luciferase...luciferase. Plasmids are Gateway cloning compatible. Learn more about DULIP in our 27 Hot Plasmids from 2016...luciferase reporter. These plasmids improve over the original STARR-seq plasmids by using the bacterial ORI...otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s...luciferase expression plasmids; these are noted with the viral service icon . ID Plasmid Luciferase Type Promoter.... ID Plasmid Description Gene/Insert PI Return to Top Do you have suggestions for other plasmids that ... luciferase plasmids used for assays of transcriptional regulation and biosensors. Plasmid...

...Cell-Imaging Plasmid Collection New England Biolabs Cell-Imaging Plasmid Collection Technology Plasmids Supplemental...needed for use of these tags. Plasmids Addgene ID Plasmid Substrate Feature 101137 pSNAP-tag (T7) Vector SNAP-tag...Control Plasmid SNAP-tag Control for nuclear localization 101123 pSNAPf-ADRβ2 Control Plasmid SNAP-tag...Control Plasmid CLIP-tag Control for mitochondrial localization 101125 pCLIPf-NK1R Control Plasmid CLIP-...Control Plasmid ACP/MCP-tag Control for cell surface localization 101128 pACP-ADRβ2 Control Plasmid ACP/MCP-tag...Biolabs has deposited plasmids at Addgene to generate SNAP, CLIP, ACP or MCP tagged plasmids for visualization...localization 101129 pSNAPf-Cox8A Control Plasmid SNAP-tag Control for mitochondrial localization 101124 pSNAPf-H2B...

...Institute for Cell Science Plasmid Collection Allen Institute for Cell Science Plasmid Collection You May Also...full description of how to use these plasmids, check out Plasmids for Endogenous Gene Tagging in Human...Allen Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key...Protein Guide Subcellular Localization Background Plasmids Protocols Background The Allen Institute for Cell...induced pluripotent stem cell (hiPSC) lines. The plasmids used to generate these lines have been deposited...distribution to the research community. With these plasmids researchers will be able to generate fluorescently...Addgene Blog. Allen Institute for Cell Science Plasmids Find additional cell line information from the...

...Khakh Discontinued Penn Vector Core Viral Vectors (Plasmids still available, other serotypes may be available...available, click on the links for more information. Plasmids are available for all the items listed below. ...pAAV.CMV.Luc.IRES.EGFP.SV40 James M. Wilson AV-9-PV1262 Plasmid not received AV-9-PV1522 100056-AAV9 pAAV.EF1a.....ChR2-YFP.WPRE.hGH Karl Deisseroth AV-9-PV1814 Plasmid not received AV-9-PV2055 105546-AAV9 pENN.AAV.CB7...-1-PV4703 101060-AAV1 pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 Eric Schreiter AV-1-PV4704 101064-AAV1 pAAV_hsyn_NES-his-CaMPARI2...Hongkui Zeng AV-2-PV0101 105530-AAV2 pAAV.CMV.PI.EGFP.WPRE.bGH James M. Wilson AV-2-PV0101 105530-AAV2 pAAV.CMV.PI.EGFP.WPRE.bGH...pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40 Eric Schreiter AV-1-PV4705 101061-AAV1 pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40...

...Broad GPP genome-wide Brunello 73179 (1 plasmid) 73178 (2 plasmid) Knockout Human Doench and Root 3rd 4 ...76,441 Broad GPP genome-wide Brie 73632 (1 plasmid) 73633 (2 plasmid) Knockout Mouse Doench and Root 3rd 4 ...GPP kinome Brunello 75314, 75315 (1 plasmid) 75312, 75313 (2 plasmid) Knockout Human Doench and Root 3rd... 3,052 Broad GPP kinome Brie 75317 (1 plasmid) 75316 (2 plasmid) Knockout Mouse Doench and Root 3rd 4 ...20,051 Human GeCKO v2 1000000048 (1 plasmid) 1000000049 (2 plasmid) Knockout Human Zhang 3rd 6 123,411...185,836 Mouse GeCKO v2 1000000052 (1 plasmid) 1000000053 (2 plasmid) Knockout Mouse Zhang 3rd 6 130,209... - 3 plasmid system 1000000075 (Puromycin) Activation Mouse Zhang 3rd 3 69,716 SAM v2 - 2 plasmid system...

... Plasmid Collections Immunology Research Immunology Research Plasmids and Resources You...Fluorescent Proteins Cancer Research Plasmids Gene Categories Plasmid Tables Additional Resources Mammalian...to the available plasmids for each gene. Please note that not all genes have plasmids available for request...and apoptosis. Plasmid Tables The gene names in these tables link to the available plasmids for each gene...Harnessing the Body’s Defenses to Fight Cancer Plasmids 101: Monitoring Cell Mobility Using Fluorescent ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene’s immunology collection contains plasmids for genes involved in innate and adaptive immune signaling...