We narrowed to 291 results for: poly

1. Finding and Joining Your Dream Lab

   Type

   Blog Post

   ...or contract, you should be starting to send out polite emails with your CV to people you’d like to work...

2. Hot Plasmids - February 2022

   Type

   Blog Post

   ...individually (~20,000 lanes/50 mLs) or through two polycistronic expression vectors (3570 lanes/50 mLs) coexpressing...

3. Antibodies 101: Buffers, Storage, and Conjugates

   Type

   Blog Post

   ...Antibodies 101: Monoclonal Antibodies Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies...

4. Antibodies 101: Isotypes

   Type

   Blog Post

   ...lot of ways to classify antibodies: monoclonal, polyclonal, scFvs, nanobodies, and the list goes on. But...

5. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

   Type

   Blog Post

   ...exciting computational algorithms, such as SIFT or PolyPhen to approach this challenge, using attributes of...

6. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

   Type

   Blog Post

   ...III. Gene III is under the control of a T7 RNA polymerase and cannot be expressed until the base editor...

7. Plasmids 101: Inducible Promoters

   Type

   Blog Post

   ...Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region ...

8. AAV Q&A with Tim Miles

   Type

   Blog Post

   ... before considering the need for some manner of polyA. In this scenario, you may be best served by a dual...

9. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

   Type

   Blog Post

   .... Using commercially available high-fidelity polymerases such as KOD and Phusion, which are recommended...

10. CRISPR 101: Non-Homologous End Joining

    Type

    Blog Post

    ...vast toolbox of processing factors, including polymerases (Pol μ and Pol λ), nucleases (Artemis), and structure-specific...

11. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type

    Collection

    ...tagging in S. pombe Insect/Baculovirus UAS, MT, Polyhedrin Targeted Gene Expression - Rubin...

12. Sweating the Small Stuff: Details in the Lab

    Type

    Blog Post

    ...at odd hours, used more secluded workspaces, or politely asked to not be disturbed when they were at the...

13. 7 Tips to Secure a STEAM Internship This Summer

    Type

    Blog Post

    ...previously mentioned, enlist the help of your network to polish the final draft prior to submission. When in doubt...

14. A Deep Dive into BioBuilder

    Type

    Blog Post

    ...whose work is focused on education and education policy. Additional Resources on the Addgene Blog Synthetic...

15. Communicating Your Science With Help From ComSciCon

    Type

    Blog Post

    ...broader impacts for their work in education and policy and to draw attention and support to work in their...

16. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type

    Blog Post

    ...to detect variants such as single nucleotide polymorphisms. Fast facts – PAM requirement: none. Best ...

17. dTAG - You're it!

    Type

    Blog Post

    ...proximity with the target protein, leading to polyubiquitination of the target protein and proteasomal degradation...

18. Troubleshooting Your Plasmid Cloning Experiment

    Type

    Blog Post

    ...companies selling PCR reagents. Nowadays commercial polymerases are very efficient, but if you are having difficulties...

19. Summer SciComm: Preprints

    Type

    Blog Post

    ...comment online! Want the general public (say, policymakers or community organizations) to use your work...

20. A Look at Addgene's QC Process

    Type

    Blog Post

    ... and biological. Even the most high-fidelity polymerases can make mistakes and lead to the accumulation...