We narrowed to 361 results for: reporter

1. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

   Type

   Blog Post

   ... where two proteins are fused to complementary reporter proteins that can only assemble when in close ... ascorbate peroxidase as a genetically encoded reporter for electron microscopy." Nature biotechnology...

2. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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   Blog Post

   ...over a decade back when Yamanaka and colleagues reported that it is possible to reprogram differentiated... blood, a huge improvement from the previously reported efforts. The pCXLE episomal system thus provides...

3. 2022 and 2023: Reflections and Looking Forward

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   Blog Post

   ... works and, equally importantly, what doesn’t. Reports receive a DOI for citation, crediting researchers...

4. Keeping Up With CRISPR/Cas9

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   Blog Post

   ...at the Broad Institute and MIT published another report showing Cas9's structural details. "Understanding...

5. Delivery Methods for Generating iPSCs

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   Blog Post

   ...high is based on the reprogramming efficiencies reported in González et al. A percentage is not listed ...comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles are removed from cells by dilution... iPSCs Find gene-specific plasmids (including reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC KLF4 LIN28...

6. How Exercise Purges Stress and Keeps Depression at Bay

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   Blog Post

   ...stress and depression, and now researchers have reported a mechanism based on studies of transgenic mice...

7. Designer PUF Proteins for Any RNA Target

   Type

   Blog Post

   ...colleagues led by Zhao described and demonstrated in a report published in The Journal of Biological Engineering...

8. CRISPR 101: Any Base Transversion Editors

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   Blog Post

   ...collaborative team from the David Liu and Dali Li labs reported a similar tool shortly thereafter, using mouse...Transversion Editors Read about four base editor reporters Resources on Addgene.org Browse CRISPR plasmids...

9. An Integrin Antibody Toolkit from IPI

   Type

   Blog Post

   ...in competition with other similar integrins, as reported by IPI and the Springer lab (Hao et al., 2024)...In this study, we, along with the Springer lab, reported the binding specificity, kinetics, and affinity...

10. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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    Blog Post

    ... the Confetti mouse (Snippert et al., 2010), a reporter mouse that can be used to track any cell type ...XFP expression in the Ubow mouse Another murine reporter animal, the Ubow mouse (Ghigo et al., 2013), was...

11. Fluorescent Proteins 101: When GFP lets you down

    Type

    Blog Post

    ...interest with an additional protein module that can report on location. This approach enables the study of...9759496.  3. Buckley, Anthony M., et al. "LOV-based reporters for fluorescence imaging." Current opinion in ...

12. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

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    Blog Post

    ...added to the repository, including beta-catenin reporter M50 Super 8x TOPFlash, scramble shRNA for use ...

13. Rinehart Lab Phosphoprotein Reagents

    Type

    Collection

    ...expression vector encoding a split mCherry fluorescent reporter, enabling the detection of phosphorylation-dependent...

14. TALENs for Endogenous Zebrafish Genes

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    Collection

    ...TALENs for Endogenous Zebrafish Gene Targets Recent reports have shown that engineered TALENs can be used to...

15. Generating Mouse Models Using CRISPR/Cas9

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    Blog Post

    ... it a single nucleotide change, insertion of a reporter gene, or replacement of the murine sequence with... R (2013) One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated...

16. Brain Initiative Collection

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    Collection

    ...plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression Edward Boyden 108912...128485-AAV9 pAAV-CAG-iSeroSnFR-Nlgn Fluorescent reporter for serotonin (dendrite localization tag) Lin ...

17. Reflections and Looking Ahead

    Type

    Blog Post

    ...viral vector serotypes, or hundreds of Data Hub reports detailing antibody experimental results. We also...

18. New Viral Vectors - Fall 2024

    Type

    Blog Post

    ...check out Addgene's Data Hub to see viral vector reports from other users. And don't forget to use the link...

19. Cellosaurus: A Cell Line Information Database

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    Blog Post

    .... For instance, recently Cellosaurus received a report that HT-55, which was described as a colon cancer...

20. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type

    Blog Post

    ...also captured as metadata.   Screen results are reported as the original quantitative gene-level scores...