What's New in CRISPR - Winter 2018
Type
Blog Post
... SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) to detect single molecules of RNA or...
We narrowed to 369 results for: reporter
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A Scientist's Guide to Ebola
TypeBlog Post... veritable virus factory. Their findings were reported in the Journal of Virology. The EBOV glycoprotein... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog Post... where two proteins are fused to complementary reporter proteins that can only assemble when in close ... ascorbate peroxidase as a genetically encoded reporter for electron microscopy." Nature biotechnology... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post...over a decade back when Yamanaka and colleagues reported that it is possible to reprogram differentiated... blood, a huge improvement from the previously reported efforts. The pCXLE episomal system thus provides... -
2022 and 2023: Reflections and Looking Forward
TypeBlog Post... works and, equally importantly, what doesn’t. Reports receive a DOI for citation, crediting researchers... -
Keeping Up With CRISPR/Cas9
TypeBlog Post...at the Broad Institute and MIT published another report showing Cas9's structural details. "Understanding... -
Delivery Methods for Generating iPSCs
TypeBlog Post...high is based on the reprogramming efficiencies reported in González et al. A percentage is not listed ...comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles are removed from cells by dilution... iPSCs Find gene-specific plasmids (including reporters and RNAi) for: NANOG OCT4 SOX2 MYC KLF4 LIN28... -
How Exercise Purges Stress and Keeps Depression at Bay
TypeBlog Post...stress and depression, and now researchers have reported a mechanism based on studies of transgenic mice... -
Designer PUF Proteins for Any RNA Target
TypeBlog Post...colleagues led by Zhao described and demonstrated in a report published in The Journal of Biological Engineering... -
The Advances Behind The World's First Personalized CRISPR Treatment
TypeBlog Post...impressive on a larger scale, too. The first paper reporting genome editing by CRISPR in mammalian cells appeared...reviewed in Lander, 2016). The first base editor was reported three years later in 2016 (Komor et al., 2016)... -
"Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science
TypeBlog Post...evaluate the resulting expression of the fluorescent reporter, the brain tissue was extracted, sectioned, and...expression from mouse to primate neocortex. Cell Reports, 34(13), 108754. https://doi.org/10.1016/j.celrep... -
CRISPR 101: Any Base Transversion Editors
TypeBlog Post...collaborative team from the David Liu and Dali Li labs reported a similar tool shortly thereafter, using mouse...Transversion Editors Read about four base editor reporters Resources on Addgene.org Browse CRISPR plasmids... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post... the Confetti mouse (Snippert et al., 2010), a reporter mouse that can be used to track any cell type ...XFP expression in the Ubow mouse Another murine reporter animal, the Ubow mouse (Ghigo et al., 2013), was... -
An Integrin Antibody Toolkit from IPI
TypeBlog Post...in competition with other similar integrins, as reported by IPI and the Springer lab (Hao et al., 2024)...In this study, we, along with the Springer lab, reported the binding specificity, kinetics, and affinity... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog Post...interest with an additional protein module that can report on location. This approach enables the study of...9759496. 3. Buckley, Anthony M., et al. "LOV-based reporters for fluorescence imaging." Current opinion in ... -
The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog Post...added to the repository, including beta-catenin reporter M50 Super 8x TOPFlash, scramble shRNA for use ... -
CRISPR Plasmids - Tagging
TypeCollection...with the following article: Natsume, et al. Cell Reports 2016 Förstemann Drosophila Cell Tagging System ... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog Post... it a single nucleotide change, insertion of a reporter gene, or replacement of the murine sequence with... R (2013) One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated... -
Reflections and Looking Ahead
TypeBlog Post...viral vector serotypes, or hundreds of Data Hub reports detailing antibody experimental results. We also... -
New Viral Vectors - Fall 2024
TypeBlog Post...check out Addgene's Data Hub to see viral vector reports from other users. And don't forget to use the link...