We narrowed to 9 results for: reporter
-
TypeGuide...promoter is typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive...vector or separate transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify...facilitates CRISPR/Cas9-based genome editing. Scientific Reports . 5 , 11315. PMID: 26066060 Zuris, J. A., Thompson...
-
CRISPR Guide
TypeGuide...and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a, it will release... using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the reaction... target sequence, it promiscuously cleaves the reporter as well, and a quantifiable signal is produced...all-in-one CRISPR/Cas9 vector system. Scientific Reports , 4 (1). PMID: 24954249 Shechner, D. M., Hacisuleyman...mapping multiprotein complexes in human cells. Cell Reports . 13 (3), 621–633. PMID: 26456817 Doench, J. G.... -
Chemogenetics Guide
TypeGuide...control expression of a chemogenetic reporter ensures that the reporter remains silent until a cell or tissue-specific... -
Guide to Using Pooled Libraries
TypeGuide...die or do not pass selection (in the case of a reporter) Sort “winning” cells Sequence those cells that... -
Molecular Biology Reference
TypeGuide...information on CRISPR check out Addgene’s CRISPR guide . Reporter Plasmids - Used for studying the function of genetic... genetic elements. These plasmids contain a reporter gene (for example, luciferase or GFP) that offers... -
Gamma-Retroviral Vector Guide
TypeGuide...the only type of selectable marker. Fluorescent reporters, such as GFP, are another common selection marker... -
Optogenetics Guide
TypeGuide...optical switches Sensors are genetically-encoded reporters of molecular signals; e.g., calcium indicators... -
Adenovirus Guide
TypeGuide...Adenovirus Vector as a Zika virus vaccine . Scientific Reports, 10 (1), 3613. https://doi.org/10.1038/s41598-020... -
Lentiviral Vector Guide
TypeGuide...the only type of selectable marker. Fluorescent reporters, such as GFP, are another common selection marker...