...antibodies (rAb) encoded by mammalian expression vectors, human embryonic kidney cells are transfected with...Neuromab Protocols (Link opens in a new window) page. Storage and Shipping Stability Addgene does not recommend... suggests preparing single-use aliquots before storing at -20 °C. Each antibody catalog item is tested...some of our other resources to help you choose, store, or use your antibody. Antibody Guide Antibody Protocols...

...s reverse complement for cloning into the pX330 vector using BbsI restriction enzyme ( Table 3 ). Add ...sgRNA expression from the U6 promoter of the pX330 vector is enhanced by the inclusion of a G nucleotide ...Prepare a 50 μl reaction mix: 100 ng circular pX330 vector, 1.0 μl annealed oligos (1 μM; see step 3.1.4 )... ( e.g., with a PCR cloning kit) into a plasmid vector. Transform the plasmid into DH5α E. coli cells ...

...Modular Assembly method Nuclease Expression Vectors - Cloning vectors for expressing zinc finger FokI nuclease...proteins for distribution to academic and nonprofit laboratories for research and educational use. Links to additional...

...are expressed and purified from plasmids in our repository....summarize antibody production and storage techniques, and explain common applications. View...some of our other resources to help you choose, store, or use your antibody. Antibody Blog Posts Recipient...

...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...catalytically dead Cas13b creates a programmable RNA editor that converts adenosine to inosine in RNA. Since...effects of ADAR2 by destabilizing its RNA binding. Editors carrying the delta-984-1090 ADAR2 truncation retain...

...and Cas9 expression vectors and validated the use of RNAs produced by these vectors for efficiently modifying...oligonucleotides needed to construct gRNA expression vectors. More information about RGNs and CRISPR-Cas systems...

...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...tagging of dCas9 can be achieved by fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin ligase... a specific locus, avoiding known transcription factor and other protein binding sites. Browse, sort, ...

...gRNA expression vectors available from Addgene....-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...experimental use of these plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions for...

...Collection of adenovirus shuttle vectors and strains...Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are a class of medium-sized...double-stranded DNA viruses known to cause mild to severe respiratory disease in humans. Advantages of adenoviruses...

...Kasparov 1732 pCPRDEnv 2nd Packaging part of the FELIX vector system, expresses Gag-Pol and Rev Nolan 35617 pCAG-Eco...promoter; CMV-EGFP reporter cassette is included to monitor expression Parijs 11619 pLB 3rd Modification of...promoter; CMV-EGFP reporter cassette is included to monitor expression Kissler 1730 pLionII 3rd FIV-derived...