Cloning
Type
Guide
...shuttled into any compatible Gateway® Destination vector, which contain attR sites via LR clonase enzymes...a simple and efficient cloning method, gateway vectors and recombination enzymes can be quite expensive...comes from a pre-cut, linear, cloning ready TOPO® vectors that has a DNA topoisomerase I fused to the 3’ ...ready, and it is not feasible to create a TOPO® vector yourself. Additionally, the efficiency can vary...overhangs of 10-12 base pairs on the 5' end of both the vector and insert. These overhangs can easily anneal creating...primers for the insert, based on the destination vector sequence and choice of restriction site. Because...