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We narrowed to 653 results for: EPO

Showing: 101 - 110 of 653 results

  1. Hot Plasmids - August 2020

    Type
    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll find in...the hPSC genome using AAV1 and contain an EGFP reporter. Expression of the dCas9-KRAB activator or dCas9...promoter. The gRNA piggyBac plasmids include a mRFP reporter. With this system, they could quantify and track...dCas9 targeting plasmids! Hazelbaker et al., Sci Reports. 2020. https://doi.org/10.1038/s41598-020-57500...

  2. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ... plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  Here's ...gene disruption in M. abscessus  Bioluminescent reporter of mechanical forces   Epitope tag antibody ...Western Blot for Anti-V5 [IPI-SV5-Pk1]. Addgene Report, https://doi.org/10.57733/addgene.l0ulu4. Hanke...doi.org/10.1128/jb.00335-23.  Bioluminescent reporting of mechanical forces with PILATeS By Emily P. ...flexible! Alex Dunn’s lab and collaborators have deposited a new mechanosensor called PILATeS, Pulling-Induced... reaction (Figure 5). The researchers used the reporter to visualize biologically relevant forces at focal...

  3. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ... plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles. Here's what... Bentley The David Liu Lab has made two recent deposits focused on optimizing prime editing systems for... PE-eVLP plasmids here! The lab’s other recent deposit builds on Prime-editing-Assisted Site-Specific ...Integrase Gene Editing (PASSIGE), their previously-reported technique for precisely integrating large DNA ...The Weissman and Gilbert Labs have previously deposited their CRISPRoff system — a programmable epigenetic...effectors (TALEs), with fifteen total variants deposited with Addgene. These variants, including a “miniCRISPR...

  4. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    ...scientists Benefits to depositing scientists: Access to a list of papers citing your deposited plasmids Increased...put, if the authors of a "Citing Article" have deposited plasmids associated with this "Citing Article"...cloning their gene of interest into a previously deposited vector - then you'll find the plasmids listed ...like to contribute plasmids you have made to the repository, email us and we'd be happy to help. Read More...

  5. Technical Design of a Western Blot

    Type
    Blog Post
    ...primary antibody required. Reporters There are two main classes of reporters: chemiluminescence enzymes...you need a specific conjugate, and changing your reporter molecule requires either re-conjugating or re-...-purchasing your primary antibody with the new reporter. Finally, each new conjugate will have to be tested...film is only compatible with chemiluminescence reporters and requires access to a darkroom and a developer... time; higher background Chemiluminescence reporter Smaller dynamic range; no multiplexing Protein-based... time; higher background Chemiluminescence reporter Smaller dynamic range; no multiplexing Wet...

  6. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    ...European scientists have deposited their plasmids into the Addgene repository. More than 4,500 plasmids...answer your questions about Addgene, plasmids, and depositing (all while our US counterparts are still sleeping...plasmids have already been deposited at Addgene by over 250 scientists in Europe. Despite these fantastic numbers...aware of what Addgene can do for them and how the repository can help them share their materials. The new ...

  7. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ...viral vectors in the Addgene repository  Name Serotype Category Depositor  Notes AAV-GfaABC1D-Cre-...new preps available in the Addgene viral vector repository! This includes the first Addgene prep packaged...the Jonathan Ting lab at the Allen Institute, deposited as part of the BRAIN Armamentarium Collection....check out Addgene's Data Hub to see viral vector reports from other users. And don't forget to use the link...

  8. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...plasmids and the lab eventually deposited these plasmids at Addgene. While deposits from for profit organizations...Officer at KSQ Therapeutics and is an Addgene depositor. KSQ uses a CRISPR-Cas9 platform to systematically...can be very impactful. At Novartis, Stegmeier deposited the ClonTracer Barcoding Library, a library that...remarkable. I was compelled to be part of that.” He’s deposited AAV constructs for gene delivery. In 2018, he ...

  9. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...double-stranded break. The Xiao Wang Lab has recently deposited a set of plasmids that helps identify base edited...plasmids use “Cas9-mediated adenosine transient reporter for editing enrichment”, or XMAS-TREE, to identify...CRISPR plasmids are always being added to the repository. To find all of the CRISPR plasmids available...

  10. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    ...Allele Biotechnology which allows researchers to deposit plasmids containing the fluorescent protein mNeonGreen...fluorophores from Allele Biotechnology that now can be deposited at Addgene. What makes these fluorescent proteins...enhanced GFP (EGFP)-type termini. mNeonGreen was reported as the brightest monomeric green or yellow fluorescent... or for live cell imaging of fusions and FRET reporters. mTFP1 is also insensitive to physiologically ...to share with the scientific community, please deposit the plasmids at Addgene! References Ai, Hui-wang...
Showing: 101 - 110 of 653 results