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...Parasite Resources Fully functional Cas enzymes will introduce a double-strand break (DSB) at a specific location...frameshifts, creating loss of function alleles. To introduce specific genomic changes, researchers use DNA ... tables below for CRISPR plasmids designed to introduce a DSB. To learn more about NHEJ, HDR, and other...

...find current pricing information Standard Quality Control Plasmid verified by next generation sequencing ...Plasmid Description Industry PI FAQ What Quality Control analyses are performed on my cloning grade DNA ... and 260/280 ratio has been determined by spectrophotometer. Other methods of quantification may yield...

...blocks the progression of the RNA polymerase, thus strongly decreasing the expression level. Mismatches between...target sequence lower the repression level. By controlling the number of mismatches we can obtain a range...reporters, mCherry and sfgfp in this case, can be controlled using a plasmid‐borne CRISPR array coding for...

...plasmids are in a lentiviral backbone, with some retroviral and AAV backbone options. Plasmids for constitutive...protocols Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors Toolbox Optimized shRNA design ... in a new window) . Wiznerowicz, M., Szulc, J., Trono, D. (2006). Tuning silence: conditional systems ...

... packaging capacity, high efficiency in driving strong transient gene expression in both dividing and ...pShuttle-CMV-F2A-T2A-Venus For production of viruses with multicistronic expression and a Venus tag Wang 50959 ShuttleMCP...-CMV-F2A-T2A For production of viruses with multicistronic expression Wang Return to top Adenoviral Plasmids...

...HIV-based lentiviral particles Murine leukemia-based retroviral particles Vesicular stomatitis virus particles...Description Mutations Industry PI Due to export control regulations, MERS and SARS-CoV materials are available...Lentiviral Envelope and Packaging Plasmids Popular γ-Retrovirus Envelope and Packaging Plasmids Reporter Plasmids...

...apamycin (mTOR) is a key metabolic regulator controlling cell growth and proliferation. mTOR, a serine...in cancer promotes the conditions needed for uncontrolled cell growth and proliferation, including an ...Kris Wood) References mTOR signaling in growth control and disease. Laplante M, Sabatini DM. Cell. 2012...

...CRISPR Read Addgene's CRISPR Guide for a general introduction to CRISPR technology. Addgene's worm CRISPR ...silencing in the germline. A "FLP-Out" system for controlled gene expression in Caenorhabditis elegans. - ...Hubbard Lab. Plasmids for the temporal and spatial control of gene expression in C. elegans by combining expression...

... CRISPR/Cas9 constructs using a high efficiency intron-optimized SpCas9-coding gene in plants. CRISPR ...Kate Creasey Krainer , Vladimir Nekrasov , Nicola Patron Additional Genome Editing Resources Check our Plant... and experimental resources that relate to the control of gene expression in the grasses. The TapRoot ...

...the location and type of MECP2 mutation can be a strong predictor of symptom severity. Broadly, early gene... in a new window) RSRT iPSC lines and isogenic controls , patient-specific Infinity BiologiX - RSRT cell...refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology or the mammalian CRISPR...