We narrowed to 641 results for: ato

...to always look closely at the trace file (or chromatogram) of the sequencing results you get back from...facility. When it comes to DNA sequencing the chromatogram is your visual control. And, like all controls...controls, missing out is a big mistake. Chromatograms come to the rescue for analyzing and troubleshooting...But look closer, the overlapping peaks in the chromatogram suggest the results are not as certain as the... of the following programs to view your .ab1 chromatogram file 4Peaks (Mac) SnapGene Viewer (Mac/PC) ...peak, don’t be shy. Feel free to edit it. Most chromatogram viewing programs (even the free ones) allow ...allowing us to share it with our readers! The chromatograms in this article were created with SnapGene. ...

...mDlx-GFP, hDlx-FLEX-GFP, hDlx-Flex-dTomato, mDlx-GCaMP6f, hDlx-GiDREADD-dTomato and mDlx-ChR2-mCherry. Find... a cell. The Goedhart lab, Gadella lab, and collaborators have an ongoing effort to improve genetically...by one-step immobilized metal ion adsorption chromatography (IMAC) using Ni-NTA magnetic beads. Workflow...multiplexed gRNAs and of dCas9 fusions. The dCas-KRAB activator and the dCas-VPR repressor are introduced into...an EGFP reporter. Expression of the dCas9-KRAB activator or dCas9-VPR repressor in hPSCs is also driven...expression. This includes a CRISPRi-based synthetic oscillator, bistable network, stripe pattern-forming incoherent...calcium sensors (jYCaMP1), variants of the calcium indicator jGCaMP7, that are optimized for excitation wavelengths...

...controlled by still other regulatory factors: enhancers, boundary elements, insulators, and silencers; however...tetracycline operators. Transcription can be turned on or off depending on what tet transactivator is used....the promoter (RNA polymerase binding site) and operators (response elements). Promoters are about 100 to...expression Promoter from T7 bacteriophage plus lac operators Negligible basal expression when not induced. ...RNA polymerase, which is also controlled by lac operator. Can be induced by IPTG. Commonly found in pET...pET vectors. Very tightly regulated by the lac operators. Good for modulating gene expression through varied...

...proteins for distribution to academic and nonprofit laboratories for research and educational use. Links to additional...

... introduced, stochasticity will result in a combinatorial expression of these three genes with different...inherently low. This is because the stochastic and combinatorial expression of fluorescent proteins is only possible...original method, we used the Tetracycline trans-activator system to boost the expression levels, and we ...we named the system Tetbow (Tetracycline trans-activator Brainbow) (Sakaguchi et al., 2018) (Figure 1)....sensory systems focusing on the functional and anatomical maturation of the olfactory system.      Takeshi...University. His lab has been studying functional and anatomical aspects of sensory circuit development using ...Livet, Jean, et al. "Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous...

...family of at least 17 DNA binding transcriptional regulators with diverse roles in processes such as cell ...

...fully-equipped laboratory for citizen scientists. In addition to maintaining a laboratory to support both... of independent Do-it-Yourself (DIY) biology laboratories working towards enabling everyone to participate... set on top of filing cabinets, a rickety old incubator, a few pipettes, and some home-made equipment....Anyone can become a member of BosLab and previous laboratory experience is not required. Our members are skilled...have non-traditional scientists working in our laboratory! Don’t worry though, we make sure that everyone...a co-organizer at BosLab, a community biotech laboratory in Somerville MA. Follow her on twitter at @ZebraElement...

...include the laboratory personnel, the serum, the cell culture media, water baths, incubators, etc. Contamination... in a single incubator since it increases the risk of cross-contamination. CO2 incubators should be cleaned...Contamination! Tissue culture media often have indicators included in the media that turns yellow from ...proliferating shortly. Once they are present in the laboratory environment, they are difficult to eradicate....tighten the flask cap before placing them in the incubators. This will also solve the problem of contamination...airflow and the lab facility periodically. The CO2 incubators, used for maintaining cell lines, must not be...

...tag neurons activated by opioid withdrawal with tdTomato and assessed cFos expression. She will explore...periaqueductal gray (PAG), with red neurons expressing tdTomato and green neurons expressing cFos. Image from ...painful stimuli are processed by a network of excitatory and inhibitory neurons whose function and connectivity...where she engineers genetically encoded voltage indicators. Tian developed a video-based high-throughput...archaerhodopsin‑derived genetically encoded voltage indicator which had improved performance in live mouse brains... Currently, she is using the improved voltage indicator coming from the screen to understand mouse cortical...Tian. Figure 5: Citrine-tagged voltage indicator expressed in mouse cortex. Image from He Tian....

...voltage indicator Article contributed by Shreya Vedantam Genetically encoded voltage indicators (GEVIs)...fluorescent optics. Existing red fluorescent voltage indicators, however, fall short compared to GFP-based sensors... sensors and blue-light-activated optogenetic actuators.  To overcome this overlap, Yiyang Gong’s lab ...linker between them. The Ace-mScarlet voltage indicator works like many FRET-opsin GEVIs. With an increase...channelrhodopsins. Find the Ace-mScarlet voltage indicator plasmids at Addgene! Beck et al., Sci Report 2019...cyanobacteria. This kit contains 96 parts including terminators and promoters, CRISPRi parts, selectable markers...