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We narrowed to 728 results for: STO

Showing: 301 - 330 of 728 results

  1. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...specific base sequences, but are sensitive to distortions in helix shape (22). Large purine:purine mismatches...enzymes.     References 1. Shuman, Stewart, and Christopher D. Lima. "The polynucleotide ligase and RNA capping...Jayakrishnan, Pravin A. Nair, and Stewart Shuman. "Last stop on the road to repair: structure of E. coli DNA ...

  2. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...Meselson-Stahl, Hershey-Chase...Who was I to dispute history? And boy did I make the right decision. If I’d ...performing quality control, and providing technical customer service. Team members are highly trained, and ...Jefferson dined alone.” There was a time in human history when an intelligent, motivated person really could...

  3. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ... viruses were produced in high-titer retroviral stocks generated by the transfer of a retrovirus into ...gene (packaging plasmid), and 3) the vesicular stomatitis virus G protein (VSV-G) that improves the stability...24h after transfection. This supernatant can be stored at 4 °C or can be directly added to your target...

  4. Antibodies 101: Validation

    Type
    Blog Post
    ...This is a good option for applications like immunohistochemistry on human tissues, when (1) other strategies...correspond to protein levels, so you perform immunohistochemistry with your antibody on these tissues (B)....mean it’s a bad antibody but more that it’s misunderstood and it could work great in another context! ...

  6. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...workhorse yeast — K. phaffii (formerly known as Pichia pastoris). If your goal is protein isolation, then the ...more MoClo? Check out Addgene's MoClo Guide! Customizing MoClo for your needs As you probably realize ...et al. “A Modular Toolkit for Generating Pichia Pastoris Secretion Libraries.” ACS Synthetic Biology, vol...

  7. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...Antibodies can be used to visualize tissues (immunohistochemistry) or cells (immunocytochemistry). This could...bind to and inhibit the function of a protein to stop its effect within the body, or they can be used ...Arbabi-Ghahroudi M (2017) Camelid Single-Domain Antibodies: Historical Perspective and Future Outlook. Front Immunol...

  8. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...leads to the first base transversion editor The story of base transversion editors starts with researchers...the SOX2 activation domain (SAD), which recruits histone acetyltransferase to open chromatin, improved editing... newly synthesized, templated sequence. And the story still isn’t over! Keep an eye out for our next post...

  9. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...creating flanking overhangs. These overhangs can be customized to link together multiple fragments, allowing...1x4; for 6 gRNAs, you’d use pX330A-1x6. This customization means you don’t ever need to clone in filler...plasmids allow expression of 2-5 gRNAs in zebrafish. Custom destination vectors are used depending upon the...

  11. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    ...Addgenie Eric Perkins attended the recent Keystone Meeting "Precision Genome Engineering and Synthetic...www.genome-engineering.org and http://www.egenome.org." At the Keystone Meeting Eric presented a poster with data comparing...

  12. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...including pseudorabies virus type 1, vesicular stomatitis virus (VSV; Beier et al., 2011), adenovirus, ...mechanisms of trans-synaptic trafficking are not fully understood and may be a form of transcytosis where the AAV...transsynaptic labeling of CNS neurons with vesicular stomatitis virus vectors. Proc Natl Acad Sci USA 108, 15414...

  13. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    ...elements from rabies plasmids to international requestors. We also offer export license applications for...processes, don’t hesitate to contact our friendly customer support team at [email protected].  Curious about...

  15. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    ...also take a look back on some fascinating Addgene history that you might not have known about. Here's the...Throwback Thursday (Blue Flame awards and Addgene history) Friday: Wrap up! To show the magnitude of plasmid...

  16. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...tradition in which we choose our teams and start brainstorming months in advance. Our teams comb the news (...year. They showed us their dedication to great customer support by dressing up as our Zendesk email system...

  17. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    ... edged passed our one million plasmids shared milestone by ending the day with 1,000,002 plasmids shipped...While we are very excited about reaching this milestone, it wouldn’t have been possible without the scientists...

  18. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...our viral vector service Addgene has offered in-stock viral vectors since 2016, with the goal of providing...production and quality control processes as our in-stock service: ddPCR, purity testing, and sterility testing...

  19. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    ...total requests, total countries represented by requestors, year of first reagent sent, year with most reagents...Therefore, users with some knowledge of R can further customize the dashboard to their needs. I hope you find ...

  20. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ... This collection of agar art from Nika Heijmans stood out to the Addgenies. Happy holidays!🎄☃️ More... work always gets done this time of year 😁 we stopped working on our reports/lab stuff for a few minutes...

  21. A Scientist's Guide to Ebola

    Type
    Blog Post
    ...the virus itself that kills you, but the cytokine storm it ultimately sets off.  The virus works its way...way into immune cells in ways that aren’t fully understood. Much of the action depends on a surface glycoprotein...

  22. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    ... 6,000 biosensors plasmids. Our most exciting milestone of 2022, however, is arguably the launch of our...have many successes to reflect upon, a wealth of history to learn from, and a compelling future to plan ...

  23. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...fellow blogger Caroline LaManna put it well: "Wow, custom mutations in monkeys already. I know we've discussed... move so fast." On the very same day the monkey story hit the presses, another very different bit of CRISPR...

  24. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...buffers. The competent cells are then aliquoted and stored at -80oC until we are ready to perform transformations...competent cells are good for at least a year when stored at -80oC.   For each new batch of competent cells...

  25. Science Communication for Everyone

    Type
    Blog Post
    ...medical conditions, so these topics have become a cornerstone for The Addictive Brain. Our community has also...Keep your message to the main three points of your story. Interested in learning more? Visit their website...

  28. Scientist Networking: What is an Informational Interview?

    Type
    Blog Post
    ... you ask for an Informational Interview it is understood to be a nonthreatening request to discuss experience... people to connect with you and to share their stories. Thanks to the Benjamin Franklin Effect, most people...

  29. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    ...social context. Episodes are driven by narrative and story telling. Chapter 5: The SITN Blog - Vini and Amy...about some of SITN's other initiatives, email sitnboston@gmail.com. Many thanks to our guests, Vini Mani...

  30. Science as Art

    Type
    Blog Post
    ...adapted to inspire to a new generation. Daniel Stoupin is a PhD scientist and photographer based at the...gathering momentum, including in Cambridge and Bristol. If you’re a scientist who spends many hours at...
Showing: 301 - 330 of 728 results