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We narrowed to 13 results for: STO

Showing: 1 - 13 of 13 results

  1. Antibody Guide

    Type
    Guide
    ...antibodies can be stored at 4 °C for short-term storage. For long-term storage they should be stored at -20 °C... Antibody Handling Storage Check the manufacturer’s instructions for exact storage requirements. As a ...tips for storing your antibody: Antibodies conjugated to fluorophores should never be stored below 4 °...define key terms, summarize antibody production and storage techniques, and explain common applications. Science...complete. HRP-conjugated antibodies should not be stored in sodium azide, as it acts as an inhibitor to ...location. Includes: Immunofluorescence (IF) Immunohistochemistry (IHC) Immunocytochemistry (ICC) Cell Sorting...in these assays. This method is suitable for DNA:histone modifier interactions, which have strong binding...

  2. Modular Cloning Guide

    Type
    Guide
    ...protein secretion in yeasts S. cerevisiae and P. pastoris (a.k.a. K. phaffii ). POMBOX MoClo YTK Yeast Expression...toolkit for protein expression and secretion in P. pastoris (a.k.a. K. phaffii ). GoldenPiCS Kit Yeast Expression...plasmids for advanced strain engineering in P. pastoris (a.k.a. K. phaffii ), including overexpression...cellulose-producing Komagataeibacter species. Ralstonia-GG-Kit Bacterial Expression Thomas Lahaye Plasmids...knockout and complementation constructs for use in Ralstonia solanacearum . XanthoMoClo Xanthobacter Genetic...MetClo DNA Assembly Kit Bacterial Expression Christopher A. O'Callaghan Toolkit for assembling large DNA...Bacterial Expression Huimin Zhao Plasmids to assemble custom-made RNA-binding proteins based on the Pumilio ...

  3. Lentiviral Vector Guide

    Type
    Guide
    ...VSV-G due to wide infectivity) VSV-G (vesicular stomatitis virus G protein) is the most common envelope ...supernatant containing the viral vectors is removed and stored or centrifuged to concentrate. You can use crude...line protocol . Browse Addgene's collection of in-stock lentiviral preps . CRISPR Genomic Screens Genome-wide...on addgene.org Lentiviral Plasmids Collection In-Stock Lentiviral Preps Addgene's Viral Prep Service Viral... The viral envelope gene; typically vesicular stomatitis virus G glycoprotein (VSV-G), an envelope protein...

  4. Molecular Biology Reference

    Type
    Guide
    ...bacterial cells that have been preserved as glycerol stocks. Function in many species — Plasmids can drive ...inoculating bacterial cultures . Antibiotic Recommended Stock Concentration Recommended Working Concentration ... acid. There are 20 amino acids plus a start and stop codon. Below you will find helpful resource tables..., UAC Valine Val V GUU, GUC, GUA, GUG Start AUG* Stop UAG (amber), UGA (opal), UAA (ochre) *AUG is the...

  5. Molecular Cloning Techniques

    Type
    Guide
    ...their recognition site allows for the creation of custom overhangs, which is not possible with traditional...have utilized this approach to create compatible custom overhangs that can then be efficiently assembled...yeast the fragments of DNA to be fused along with custom synthesized DNA oligos that span each junction....

  6. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...VSV-G due to wide infectivity VSV-G (vesicular stomatitis virus G protein) is the most common envelope ...supernatant containing the viral particles is removed and stored or centrifuged to concentrate. Crude or concentrated... The viral envelope gene; typically vesicular stomatitis virus G glycoprotein (VSV-G), an envelope protein...

  7. Plan Your Experiment

    Type
    Guide
    ...using CRISPR . Browse Addgene's collection of in-stock lentiviral preps . AAV vectors AAV vectors offer...overcoming the AAV size limitation . Browse Addgene's in-stock AAV preps or try Addgene's Packaged on Request service...paired with restriction enzymes for further customization Usually paired with a sequencing method Can’...

  8. CRISPR Guide

    Type
    Guide
    ...insertions, or frameshift mutations leading to premature stop codons within the open reading frame (ORF) of the... cytosines in a gene’s promoter or by inducing histone acetylation or demethylation. The most common modifiers... green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence microscopy...gene by shifting the ORF and/or creating premature stop codons NHEJ N on- H omologous E nd J oining; A DNA...

  9. Promoters

    Type
    Guide
    ...factors and histones (proteins that package DNA into nucleosomes) also bind the TATA box. Histone binding ...

  10. Chemogenetics Guide

    Type
    Guide
    ...phospholipase C, which releases intracellular calcium stores. There are currently three Gq DREADDs based on ...doi.org/10.1016/j.neuron.2015.03.065 PMID: 25937170 Weston, M., Kaserer, T., Wu, A., Mouravlev, A., Carpenter...

  11. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...capsids for more information. Browse Addgene’s in-stock viral preps . AAV retrograde (AAVrg) Directed evolution...Packaging Services Packaged-on-Request AAV Preps In-Stock AAV Preps Resources on addgene.org AAV Plasmids ...

  12. Educational Resources

    Type
    Guide
    ...Transformation Plasmid Cloning Creating Bacterial Glycerol Stocks Restriction Digests Guides Browse our in-depth ...

  13. Optogenetics Guide

    Type
    Guide
    ...cells. LITE Two-hybrid system integrating the customizable TALE DNA-binding domain with cryptochrome 2 ...
Showing: 1 - 13 of 13 results