We narrowed to 768 results for: Vars

... is composed of identical repeat sequences and variable spacer sequences. The array is transcribed and...in the non-target strand of DNA to be cut, which varies between CRISPR systems. Cas proteins won’t cut ... CRISPR by mutating these sequences. Pro tip! Various labs have created Cas9 enzymes with looser PAM ...

...Professor at the Lab for Innovation Science at Harvard, wanted to see how new tools drive innovation in... their gene sequence may have slight or large variations depending on how closely related these species...percentage were not good predictors. There is not much variation in GC content between many organisms and DNA synthesis...

... confirm expression in target cells. While the various AAV serotypes can be used to achieve different ... plasmids now packaged in AAVrg. jGCaMP7s, the variant with the most sensitive response to action potentials...to measure glutamate levels. iGluSnFR.A184S, a variant with higher affinity to glutamate, has been packaged...

...Streptomyces, a prokaryotic genus capable of producing a variety of natural products. Recently, this group optimized...base-editing efficiency using XMAS-TREE-based methods at various genomic loci in four different categories. ...  Various Addgenies...

...Polyclonal antibodies produced in animals may be variable among animals and bleed dates. The development...as other affinity reagents such as single-chain variable fragment (scFvs) and nanobodies. Addgene will ...Hybridomas frequently express additional functional variable regions. mAbs 10:539–546 . https://doi.org/10.1080...

...pH-insensitive RFP variant, DsRed.T3, that’s connected to a pH-sensitive GFP variant, super ecliptic pHluorin...fixed cells. Find the mitochondria-targeted Keima variant at Addgene! mRFP-GFP-LC3 tandem fluorescent protein...

...to the beat of their own drum. They have a wide variety of possible genome types that can be either double...positive sense ssRNA, or negative sense ssRNA) and varying methods of replication. In fact, an entire classification...proteins. RNA viruses replicate their genomes using a variety of RNA or DNA intermediaries, depending on the ...

...cilia. They’ll put a variety of plasmids to work to explore the effect of various OCRL1 mutations on cell... with CRISPR-Cas9. There are so many different variants and strategies out there. This will help us acquire...

...never completely eliminate the risk that a minor variation in experimental conditions will derail a research...and why other publishers and organizations have various different versions of checklists and guidelines...Elsevier after her postdoctoral studies, and has held various editorial roles on review and primary research ...

...blogger Talley Lambert, a Research Associate at Harvard Medical School. The need for a community fluorescent...Joachim Goedhart on the Addgene blog – outlining various important considerations when choosing a FP, but...Lambert! Talley Lambert is a Research Associate at Harvard Medical School. He manages the Cell Biology Microscopy...

...researchers have been able to study the effects of various stimuli on global translation, but a visual technique...second. They also explored the effects of 5’ UTR variants in translation of cell cycle protein Emi1, showing...represent powerful tools for studying translation in various cell types and physiological states. References...

...used N- and C-terminal dronpa fusions. With these various designs, Xin has been able to develop photoswitchable...has demonstrated the utility of these FLiPs in a variety of research applications and particularly notes...be able to use similar designs to create a wide variety of enzymes and proteins with inducible activity...

...resistant to detergents and degradation under a variety of conditions. They are composed of 4 proteins ...robust linear actuators that could be used in a variety of applications. For example, you could use them...Silver in the department of Systems Biology at Harvard Medical School. Follow her on Twitter @jessicapolka...

...Institute today, where he helps researchers in a variety of fields develop RNAi screens to answer their ...more about cancer biology in Ed Harlow’s group at Harvard Medical School and then applied to work at the ...Genetic Perturbation Platform and encompasses a variety of technologies to functionally investigate the...

...London), Dr. Connie Cepko (Harvard Medical School) , and Dr. George Church (Harvard Medical School) for taking...and writer.” Connie Cepko was surprised by the variety of skill sets she needed to acquire as a professor...

...London), Dr. Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) for taking...throughout the year since hiring opportunities arise at various times. If the timing and interests align for both...

...degenerate primer set to amplify IgG variable light (VL) and variable heavy (VH) chain sequences. Fusion...thoroughly validated. The creativity in employing a variety of cloning techniques and troubleshooting methodologies...

... there!), think of ways you could change your variables - your approach, your workflow, or your physical...prevent the mistake, just like you would change variables to fix a failed experiment.  For example, I used...to reduce the chances of making an error. Try a variety of things, including (and maybe especially) suggestions...

...mammalian expression vectors containing various spike protein variants are also available. Plasmids are continually... Various Addgenies...

...pressure to select for variants of interest, though. A selection can take the form of varied temperature, nutrient...help determine which is right for you. Screen variations Forward and reverse screens Forward and reverse...

...continuation of that work, they have found at least two variants that display increased neuronal expression in ...side effects, by allowing the user to select for variants that infect the CNS while selecting against those...subclones within a single well reduced well-to-well variability, while the readout was able to be deconvoluted...

...scale by screening diverse libraries of genetic variants. Several methods for generating mutant libraries...provides a new approach for generating novel genetic variants in bacteria. Read on to learn about the key components... other three bases, which limits the level of variation that can be introduced.   Diversification is...

...RNA-protein interactions. To address this need, the Khavari lab at Stanford created the RNA-protein interaction...disease. RaPID can also be used to test how RNA variants associated with human disease affect protein binding...Siprashvili, Z., Palmer, T.D., Carette, J.E., & Khavari, P.A. (2018). RNA–protein interaction detection...

... application. Consider also that there may be variations within an application. Take immunohistochemistry...might find that while the website highlights a variation of your application, a publication successfully...this is the case, you can narrow them down in a variety of ways. First, review the citations. An antibody...

...editing in both bacteria and human cells using various delivery methods, including plasmid, ribonucleoprotein...efficiency of prime editing to correct mutations in a variety of target genes and cell types. Their Nature Biotechnology... Various Addgenies...

...Filip Tomaska and Tim Hanson, are characterizing variants of iGluSnFR3 in vivo and have used it to detect...these cells and the functional contribution to a variety of behaviors related to addiction and pain. Once...biosensors He Tian (goes by “Tian”) is a postdoc at Harvard University where she engineers genetically encoded...

...model, ubi:zebrabow. In this system, cells express various fluorescent proteins controlled by Cre recombination... that recombine independently and results in a variety of colors depending on the combination of fluorescent... Various Addgenies...

...from countless rounds of optimization. Dozens of variants exist with unique cutting features and evolved...engineering endeavors. For more information on Cas9 variants, check out our blog post on choosing a Cas9 enzyme... detection tool for rare, often hard to detect variants such as single nucleotide polymorphisms. Fast...

...tens of thousands of proteins functioning in a variety of ways. How do scientists focus on the activity...acid sequence, or tag, on the protein. While a variety of methods can be used to successfully isolate ...tools for studying proteins and are used for a variety of assays in the lab (for a refresher see Antibodies...

... is explained in more detail here. There are a variety of methods that can be used to measure your fluorescent...filtration (Chudakov et al, 2007). We have developed a variety of plasmids that can be used in the OSER assay ... Of course, you want the ‘latest and greatest’ variants with the best properties for your research. But...