We narrowed to 642 results for: Emb;

1. Fluorescent Proteins 101: Luciferases

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   ...as the most efficient means to disrupt the cell membrane and deliver the substrate; however, secreted luciferase...

2. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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   ...specific locus to create a knock-out in human embryonic stem cells.  Find GEmCherry2 reporter plasmids...

3. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

   Type

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   ..., spike proteins, envelope small proteins, and membrane proteins. Although all viral components are available...

4. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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   ...conducting the experiment (note: registrations can be embargoed while the research is ongoing, but all registries...

5. Hot Plasmids - February 2022

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   ... yet low-pressure teaching opportunity for lab members or students new to protein expression and purification...

6. Sweating the Small Stuff: Details in the Lab

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   ...side to your right as you use them can help you remember what's been added and what hasn't. Developing ...

7. Antibodies 101: Isotypes

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   ...tears, saliva, mucus) where it protects mucosal membranes. This isotype is most commonly a dimer with a ...

8. Improved Plasmid Maps Powered by SnapGene

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   ...colleagues, or explain plasmid features to a lab member when you’re not at your computer. Maps, sequences...

9. Luminescent Imaging with Nano-lanterns

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   ...publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns for...

10. Multiplex Genome Editing with CRISPR-Cpf1

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    ...Disadvantages References Yamamoto Lab Golden Gate Assembly transfection One vector expresses Cas9 and ...

11. Screening for Successful Genome Editing with Digital PCR

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    ... in addition to testing their performance (2). Remember that although digital PCR is a great screening...

12. Why and how to start studying chromatin (with Addgene's help)

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    ...happens, there are a lot of scientists who not only remember to think about DNA in this context, but also specifically...

13. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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    ...enzyme can help determine which enzyme to use. Remember, accessibility, specificity, and sensitivity are...

14. Viral Vectors 101: Inverted Terminal Repeats

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    ...ITRs in the plasmid backbone and do not properly assemble, but these sequences can sometimes be removed ...

15. Viral Vectors 101: Viral Applications

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    ...-transfect cell types ranging from primary to embryonic stem cells. Viral integration of a construct is...

16. CRISPR 101: Non-Homologous End Joining

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    ... works in Dale’s lab.        Dale Ramsden is a member of the curriculum in Genetics and Molecular Biology...

17. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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    ... multiple mentees efficiently Allows all group members to lead and learn at the same time Broadens the...

18. AAV Packaged on Request

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    Collection

    ...start getting results from your AAV experiments, remember to share your protocols and results through Addgene...

19. Addgene: The First Twenty Years

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    ...labhold” name. Our first plasmid request came on September 8, 2004, for pcDNA-p160MBP, while the first request...

20. Antibodies 101: Affinity Reagents

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    ...through mutation, allowing diabodies to be used for assembling protein nanostructures. Diabodies readily co-...

21. Transferable Skills Guide: Problem Solving

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    ...both in and out of the lab. The next step is to remember that you have these skills when you start applying...

22. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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    Blog Post

    ...multiplexed imaging of neurotransmitter, calcium, and membrane voltage,” says Tian. Figure 5: Citrine-tagged...

23. Choosing the B(right)est Fluorescent Protein: Photostability

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    ...in microdroplets in a water/oil emulsion, (ii) embedded in a gel, or (iii) attached to a substrate. These...

24. Viral Vectors 101: An Introduction to AAV

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    ...expression for several years (Wonjo et al., 2013). Remember that AAVs do not integrate into the host genome...

25. The time and cost required to make a plasmid

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    ... is a concern. The faster methods, like Gibson assembly, are typically more expensive, so you’ll have ...

26. Summer SciComm: Preprints

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    ...journal and share it more widely!  Not everyone embraces preprints as part of their scientific communication...

27. CRISPRainbow and Genome Visualization

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    ...GFP-dCas9 and the gRNA scaffold to enhance complex assembly, thereby decreasing the amount of background fluorescence...

28. Gendered Innovations: Why Does Sex of the Cell Matter?

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    ...analysis for innovation and discovery. She is a member of the American Academy of Arts and Sciences and...

29. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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    ... their ability to cut the EMX1 locus in human embryonic kidney (HEK) cells, 11 of these initial mutants...

30. The Future of Research Symposium Boston 2015

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    ...posing some of their own are the following panel members: Jonathan Dinman, Chair of the Cell Biology and...

31. New Tool for Lineage Tracing: The ClonTracer Library

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    ...library allows a researcher to label individual members of a starting population of cells with specifc,...

32. AAV Molecular Tools

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    Collection

    ...Syn-driven, Cre-dependent Cre-dependent expression of membrane-bound GFP and synaptophysin-mRuby for labeling...

33. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    ...splice sites. Nat Biotechnol. Published online November 5, 2018. doi:10.1038/nbt.4283 Zibitt MS, Hartford...

34. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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    ... with Cas9 interferes and prevents the correct assembly of the crRNA-Cas complex (Zhu et al., 2019). Inhibiting...

35. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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    ...Addgene as a DNA Sequencing Analyst intern. As a member of the Quality Control team, I help make sure the...

36. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    ...your standard curve to plot to a linear equation (remember y = mx+b?). Figure 5: A standard curve ...

37. Plasmids for Endogenous Gene Tagging in Human Cells

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    ...technique that briefly destabilizes the cell’s outer membrane and allows the components to physically enter ...

38. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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    ...s research is the mechanisms by which proteins assemble into functional multi-subunit macromolecular complexes...

39. Adenoviral Vector Production and Troubleshooting

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    ...packaging signal (Ψ), both of which are essential for assembly of the virion. Adapted from Lee et al., Adenovirus-mediated...

40. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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    ...considerations for Split-BioID It’s important to remember that with Split-BioID, and BioID, any protein ...

41. CRISPR in the Clinic

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    ... in clinical trials includes Cas3 (the shredder member of the Cas family) as part of a cocktail therapy...

42. Viral Vectors 101: Virus Safety

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    ...biosafety cabinet. When decontaminating liquid waste, remember that the percentage indicates the proportion of...

43. Antibodies 101: Choosing the Right Isotype

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    ..., G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical Techniques for Light and ...

44. Engaging with science and society at pgEd

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    ...’ve since returned to pgEd as a full-time staff member for the past three years. pgEd is a small team ...

45. Overcoming the Challenges of Lentiviral Production

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    ...dish can be easily applied to multiple dishes, remember that handling multiple dishes will take longer...

46. Corralling and Cornering the Goldilocks Antibody

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    ...Zhuhao Wu and his colleagues! Dr. Wu and his lab members (like me!) are dedicated to creating a public and...

47. 6 Tips for Grant Writing

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    ...about what reviewers in your area are looking for. Remember though, you need to leave them time to read it...

48. Tips for Technical Support Calls

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    ... money on a potentially faulty product. Please remember that the person at the other end of that email...

49. Open Enzyme Collection

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    ...Enzyme parts have C/E overhangs for Golden Gate assembly. Plasmids Explore the collection and order individual...

50. Hot Plasmids: Summer 2025

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    ...allows VAMP2-At to remain on the presynaptic cell membrane while AF, fused to a payload, is released into...