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  2. Scientists Map the SARS-CoV-2-Human Interaction Network

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    Blog Post
    ...distance.  From front to back: David Gordon, Jeff Guo, Fengbo Zhou, Paddy O’Leary (UCSF). Our approach is ...to Margaret Soucheray, Jeff Guo, Paddy O’Leary, Fengbo Zhou, Helene Foussard, and Dave Gordon) have been...

  4. Addgene Depositors Get More Citations

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    Blog Post
    ...from the MIT Sloan School of Management. Professor Feng Zhang’s original 2013 gene editing paper on CRISPR...References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." Science 339.6121 (...

  5. With Our New Viral Service, We're Taking CRISPR Further!

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    Blog Post
    ...the Wyss Institute for Biologically Inspired Engineering and member of the Addgene Advisory board, on ...the Wyss Institute for Biologically Inspired Engineering and the Zhang and Doench/Root labs from the Broad...

  6. Four Factors that Differentiate the Stem Cell Field

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    Blog Post
    ...lines from becoming the new HeLa cells?), genome engineering and stem cells, and the difficulty of sharing...into a pig. In stark contrast to recent genome engineering technologies, stem cell techniques are neither...

  7. 5 Great Apps for Lab Life

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    Blog Post
    ...starting cell culture work. 3. NEB Tools by New England BioLabs In my lab we do a lot of genetic construction...experiments. Not all of our lab enzymes are made by New England BioLabs, but the NEB Tools app comes in handy when...

  8. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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    Blog Post
    ... Complementary to the released strand, 127bp in length, With 36bp on the PAM-distal side of the break ...al. "Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery....

  9. Addgene Welcomes John Doench to the Advisory Board

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    Blog Post
    ...comments section below! Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.       Resources...

  10. Finding and Joining Your Dream Lab

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    Blog Post
    ...scientists and technical people) is linked to engagement and access to growth opportunities, not salary...zone when you lose track of time because you are engrossed in a task. Things seem to fall into place frequently...

  11. Viral Vectors 101: The AAV Retrograde Serotype

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    Blog Post
    ...Campus and the University of California, Berkeley engineered an AAV variant that efficiently infects neurons...recombinase-dependent vectors, this can cause challenges for the experimental design as these vectors ...

  12. Viral Vectors 101: Viral Applications

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    Blog Post
    ...typically their own genome, but this step can be engineered to be anything you want delivered to your target...and virus. Viral CRISPR-Cas9 tools have been engineered to introduce frameshift mutations in genes of...

  13. Sharing is Caring: But How to Distribute Open Hardware?

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    Blog Post
    ...routine, open-source software projects that actively engage with the community are particularly successful....hardware, this process is often much more involved. Challenges such as the provision of an accessible supply...

  14. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    Blog Post
    ...asked questions about γ-retroviral vectors How do engineered γ-retroviruses differ from lentiviruses? There...There are a few important differences between engineered γ-retroviruses and lentiviruses. First, these viruses...

  17. GPCRs: How Do They Work and How Do We Study Them?

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    Blog Post
    ...specific behaviors. Chemogenetics uses genetically engineered receptors to interact with small molecules to...Designer Drugs (DREADDs) (Roth, 2016). DREADDs were engineered from members of the human muscarinic receptor...

  18. A Novice's Perspective on Neuroscience - SfN2017

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    Blog Post
    ...Even if you're currently a synthetic biologist engineering E. coli metabolism, maybe you’ll be able to apply...Optogenetics, Neuroscience, and the Future of Neuroengineering Primer on Optogenetics Resources at Addgene.org...

  20. Multiplex Genome Editing with CRISPR-Cpf1

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    Blog Post
    ...CRISPR-Cpf1 genome editing!  A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex...spCas9: ~4 kbsaCas9: ~3 kb ~3.9kb crRNA/gRNA length gRNA: ~100 nt crNA: ~42 nt Requires tracrRNA?...

  21. Clear and Accessible Writing

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    Blog Post
    ...scientific paper, you may feel it’s overwhelming or challenging. So instead, let’s focus on practicing with a...dive into applying these four steps elsewhere, engaging in this practice, and perhaps modifying it as ...

  23. To Each HIS Own

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    Blog Post
    ...very pure sample. Because binding strength is proportional to the length of the tag, with longer tags you...

  24. All in a Twist: dsRNA

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    Blog Post
    ...abundant in normal cells and are arise from a ssRNA engaging one strand of a dsDNA molecule; the 3-stranded..., but they also have a darker side. When ssRNA engages one strand of a dsDNA molecule, it does so at the...

  25. Giving gRNAs a Facelift - Synthetic and Beyond

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    Blog Post
    ...utilizes a gRNA which is photocaged and unable to engage a target sequence. Upon a brief light exposure ...Rosenberg, M., Hendel, A. Using Synthetically Engineered Guide RNAs to Enhance CRISPR Genome Editing Systems...

  26. Antibodies 101: Fab Fragments

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    Blog Post
    ...fragment, Fvs are often produced recombinantly and engineered to be joined by a linker peptide, resulting in...Tomisaki M, Kiuchi T, Takagi J, Watanabe N (2022) Engineered fast-dissociating antibody fragments for multiplexed...

  28. Gendered Innovations: Why Does Sex of the Cell Matter?

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    Blog Post
    ...numbers of women participating in science and engineering. “Fix the Institutions” promotes gender equality...Gendered Innovations in Science, Health & Medicine, Engineering, and Environment—a project aimed at harnessing...

  30. Viral Vectors 101: Virus Safety

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    Blog Post
    ...safety features   When viruses were first being engineered as biological tools, thought was given on how...replication of virus and its infectivity in check. Engineering controls, safety systems that are built into ...

  31. Plasmids 101: The Wide World of Natural Plasmids

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    ... Eukaryota can also harbor these circular DNA passengers. Most natural plasmids encode beneficial traits...Gateway cloning. In addition, scientists have engineered entirely new types of plasmids without clear ...

  32. How to Prepare for an Industry Interview

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    Blog Post
    ... time out of their day to talk with you, so be engaged with their conversation. Send thank you emails ...carefully. And even if you don’t have technical challenges, your interviewers might.  You’ll probably still...

  34. Rabies and Neuronal Tracing

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    Blog Post
    ...and deliver a variety of cargoes, genetically engineered neurotropic viruses emerged as powerful tools...Watabe-Uchida et al., 2012). They could also quantify the strength of the inputs by counting the monosynaptic inputs...

  36. Plasmids for Endogenous Gene Tagging in Human Cells

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    ...Institute website at alleninstitute.org. A classic challenge in cell biology is making sure that what we observe...of the protein gleaned from the literature and engagement with researchers who study the protein or structure...

  38. Hot Plasmids February 2024

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    Blog Post
    ...here!   Zhang, Z., et al. (2023). Efficient engineering of human and mouse primary cells using peptide-assisted...Researchers in the Gradinaru lab have developed an engineered adeno-associated virus (AAV) vector named AAV.CAP-Mac...

  39. 5 Tips for Troubleshooting Viral Transductions

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    ...tasks, both of which are factors in the technical challenges they can present. Many researchers find themselves....2 (2003): 141. PubMed PMID: 12705861. 3. Chen, Sifeng, et al. "Freeze-thaw increases adeno-associated...

  40. Plasmids 101: FLEx Vectors

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    ...Hongkui Zeng Additional Resrouces on the Addgene Blog  Read about Other Means of Genome Engineering Using...

  42. Experimenting in an Open Source lab: from CRISPR to Cats

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    ...Makerspace is composed of computer scientists, engineers, and biologists working together on a variety ... case.   Funding Makerspaces One of the main challenges when starting a new project at a makerspace is...

  44. How-to: 5 Steps to a Great Panel Discussion

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    ...discussions with both panelists and audience fully engaged. They can also be dull and painful to sit through...well-planned approach to making the event interesting and engaging. Long before the event... Choose a moderator ...

  45. The AAVantages of AAV in CRISPR Screens

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    ...identified in nature, while many more have been engineered in labs. This vastly increases the possibilities...S. E., Muller, A., Sousa, A. A., Tsai, S. Q., Bengtsson, N. E., Lööv, C., Ingelsson, M., Chamberlain, ...

  46. Production of Virus in Insect Versus Mammalian Cells

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    ...Come From and What Do They Mean? Overcoming the Challenges of Lentivirus Production Tips for Titering Your...recombinant proteins and viral vectors. Frontiers in Bioengineering and Biotechnology, 9. https://doi.org/10.3389...

  48. Transferable Skills: Negotiation

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    Blog Post
    ...natural. Creative negotiation skills will help you strengthen relationships with your colleagues and supervisors...help you get more of what you want and can also strengthen your relationships with others who know that ...

  49. Fluorescent Tagging of Endogenous Genes with SapTrap

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    Blog Post
    ...and protein turnover rates. Additionally, by engineering FP fusions associated with cellular organelles...the tag for each insertion. Also, it remains challenging to screen for the genetically modified strains...
Showing: 501 - 550 of 801 results