We narrowed to 535 results for: try;
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TypeBlog Post...protein of interest (POI), that facilitate immunohistochemistry (IHC) experiments with tag-specific antibodies...
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Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog Post...the BDNF hypothesis of depression?." Molecular psychiatry 12.12 (2007): 1079. PubMed PMID: 17700574. Hare... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog Post... barrier (BBB). It’s the BBB’s job to block the entry of pathogens to the CNS, but this also stops viral... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post... visualizing neuronal differentiation by immunohistochemistry, it is not amenable to FACS. Thus, the ... -
Supporting Reproducibility with a Connected ELN
TypeBlog Post... into the desired repository. They also support entry of required metadata, with the option of adding ... -
Antibodies 101: Immunoprecipitation
TypeBlog Post... Basics of Western Immunoblotting.) For mass spectrometry, elute in a urea-containing lysis buffer. Alternatively... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog Post...-based biosensors.” TrAC Trends in Analytical Chemistry 27(2):108-117 · February 2008. 11. Svensen, Nina... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog Post...Initiation of Replication. Journal of Biological Chemistry, 273(14), 8447–8453. https://doi.org/10.1074/jbc... -
Viral Vectors 101: Pseudotyping
TypeBlog Post...virus is to delete the glycoprotein required for entry into the cell (rabies dG). Because rabies dG can... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog Post...neurons: differential participation in reward circuitry." Cell reports 8.6 (2014): 1857-1869. Pubmed PMID... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog Post...Single Chain Fv Protein." Journal of Biological Chemistry (2000). PubMed PMID: 10748019. 8. Lotze, Jonathan... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog Post...effective, in both academic circles as well as in industry. The news is good. In their ideal form, ELNs provide... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog Post...characterization of dedicated sensors for FLIM, for ratiometry and with high affinity." PloS one 10.4 (2015)... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog Post...gene-silencing machine. Journal of Biological Chemistry, 284(27), 17897–17901. https://doi.org/10.1074... -
Hot Biosensors 2022: Year-End Roundup
TypeBlog Post...’s headquarters, the skies are getting dark and wintry with the end of year approaching. We’re always ... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog Post...matrix metalloproteinases." Journal of Biological Chemistry 290.8 (2015): 4728-4740. You can find more info... -
Antibodies 101: The Basics of Western Blotting
TypeBlog Post...antibody and radioiodinated protein A. Analytical Biochemistry 112:195–203 . https://doi.org/10.1016/0003-2697... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog Post...study. These are highly useful for the biotech industry, which is in the process of capitalizing on phage... -
Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog Post...and high throughput state detection with flow cytometry. However, expressing reporters will consume cellular... -
Quick Guide to All Things Lentivirus
TypeBlog Post...marker can be separated by an internal ribosome entry site (IRES) and expressed under the same promoter... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog Post...work (now recognized by the 2020 Nobel Prize in Chemistry), and Victor Ambros and Gary Ruvkun for their ... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog Post...fluorescent protein into a photoactivatable probe." Chemistry & biology 12.3 (2005): 279-285. PubMed PMID:15797211... -
Typing CRISPR Systems
TypeBlog Post... the most complex prokaryotic immune system. Biochemistry (Moscow), 86(10), 1301–1314. https://doi.org... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog Post...understanding of cellular mechanisms. Advancements in dye chemistry have led to the development of more stable, brighter... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post...transgenic mice for optogenetic dissection of neural circuitry function. Nature methods. 2011;8(9):745-52. PMID... -
Promoters
TypeGuide...metabolic operon trp Repressible by tryptophan Promoter from E. coli tryptophan operon Ptac Regulated like the... The trp operon involved in the production of tryptophan is an example of a positively controlled operon... -
Cloning
TypeGuide... a proprietary BP clonase (creating an entry clone). The entry clone now has recombined attL sites flanking...reducing ligation efficiency. TOPO® ready Gateway® Entry plasmids are also available, allowing for rapid ... -
15 Hot Plasmids from 2017
TypeBlog Post...diminished KLF4 expression levels; this altered stoichiometry impacted reprogramming and global gene expression... -
Technical Design of a Western Blot
TypeBlog Post...recommend doing a little more research into gel chemistry and/or specialty gel options. Table 1: Types of... -
Adeno-associated virus (AAV) Guide
TypeGuide...Needle sticks and ripped gloves are common points of entry. Biosafety should always be considered with respect...Blood-brain barrier (BBB) Layer of cells that prevent the entry of toxins and pathogens from the blood to the CNS...packaging cell line). Infection The natural process of entry and multiplication of a pathogen, like viruses or... -
Gamma-Retroviral Vector Guide
TypeGuide...Needle sticks and ripped gloves are common points of entry. Biosafety should always be considered with respect...packaging cell line). Infection The natural process of entry and multiplication of a pathogen, like viruses or... -
Adenovirus Guide
TypeGuide...Needle sticks and ripped gloves are common points of entry. Biosafety should always be considered with respect...packaging cell line). Infection The natural process of entry and multiplication of a pathogen, like viruses or... -
Lentiviral Vector Guide
TypeGuide...Needle sticks and ripped gloves are common points of entry. Biosafety should always be considered with respect...packaging cell line). Infection The natural process of entry and multiplication of a pathogen, like viruses or... -
Viral Vectors 101: Systemic Capsids
TypeBlog Post...your strain of interest, check the mechanism of entry if known, and, as always, validate the capsid for... -
CRISPR Guide
TypeGuide...associated with the locus can be identified by mass spectrometry (proteins), RNA-sequencing (RNAs), and NGS (... & Liu, D. R. (2018). Base editing: precision chemistry on the genome and transcriptome of living cells...