...AdV surface proteins (especially fiber’s knob domain, and hexon), as well as other intracellularly-exposed...pediatric population. Once ~400 cases had amassed in places around the world, hypotheses began to develop that...AdV genome in them, including the E1 region). The space in the viral particle’s capacity that is made available... G. R. (2015). Adenovirus Tales: From the Cell Surface to the Nuclear Pore Complex. PLoS Pathog, 11(6)...

...Jonathan Gootenberg, PhD, focuses exclusively on this space — developing tools for cellular targeting. Lately...and cleaving RNA at positions defined by the target:spacer duplex without detectable non-specific activity...mutations wouldn’t matter – the mutated copy could be replaced by simply dragging and dropping in a functional...allowing a one-size-fits-all approach to gene replacement therapy for most patients. But how does one go...chance of the insert being “dropped” in the wrong place, while increasing the number of potential applications...

...vectors, you need an envelope protein on the virus’s surface and a corresponding receptor in the host cell. ...low-density lipoprotein receptors (LDLRs) on a cell surface to endocytose into the cell. The reason VSV-G is...preparation, VSV-G is stable and can withstand ultracentrifugation which helps scientists achieve a high titer... rabies glycoprotein now serves as a retrograde tracer so that scientists can identify the neurons that...2001).  This method can also allow scientists to trace a neuron’s projections back to the cell body. Rabies...

...commonly associated with CRISPR arrays and involved in spacer acquisition following infection, as a form of “...is reduced when 2 mismatches are present. The protospacer flanking sequence (PFS) for LshCas13a, which ...sequence for Cas9, is located at the 3’ end of the spacer sequence and consists of a single A, U, or C base...each DNA target have high sequence identity to the spacer sequence and contain the appropriate PAM sequence...cells. LwaCas13a-msfGFP can mediate cleavage with spacers from 20-28 nucleotides in length and does not require...

... public speaking where I enjoy communicating face to face with the audience. I post on LinkedIn where ...thoughts on why we decided to do an AMA in the first place and give you some reasons why you should consider...realized that the r/Science AMA series was the best place for us. We’re definitely glad we did - you can see...lot of links and content at hand in three useful places: I had posted content on many of the requested...

...nose-poke at the active hole [1, 2].    Place preference/place aversion also models reinforcement but ...include: intracranial self-stimulation (ICSS) and place preference. The lab I work in (the Kreek lab) focuses...ideal behaviors to test reinforcement are ICSS and place preference.   Historically, ICSS was done by implanting...hippocampal neurons are activated when the mouse is placed in a novel context. As a proof-of-principle test...

...suspended in the original media or isolated and placed in a buffer. Antibody Structure Figure 1: Structure...of 4 IgA1; IgA2 IgD Coexpressed with IgM on the surface of most immature B cells; function is unknown Monomer...by a capture antibody (sandwich). Block unused surface-binding sites in the wells with a blocking protein...sandwich ELISA, a capture antibody, bound to a surface, is used to capture the protein of interest and...In IHC, tissue sections are assayed and the extracellular matrix remains intact. The signaling molecule...enzyme. In ICC, cells have most or all of their extracellular matrix removed. Again, the signaling molecule...cells. Cells, with labeled antibodies bound to surface receptors, are “flowed” through a flow cytometer...

... to you: our first, second, and third place winners!  1st Place: Krampus Saves Christmas! created by the... Xi Chen (@XiChen7889) December 10, 2021   2nd Place: The Winter and the Falcon Tube created by the team...Heng Chooi (@YitHengChooi) December 4, 2021   3rd Place: O! Beaker Tree Created by Joanne Jary from Lab ...

...activation p300 acetyltransferase dCas9 fused to the catalytic domain of p300 acetyltransferase increases levels...modifications, researchers used blunt tools like histone deacetylases, but targeted epigenetic modification was impossible...light-regulated histone methyltransferases and deacetylases. CRISPR and epigenetics As with many TALEN-based... Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers...

...culture through various sources that are difficult to trace. These include the laboratory personnel, the serum...the flasks (unless the source of culture is irreplaceable or highly expensive). Detecting mycoplasma contamination...mycoplasmas, contamination by other bacteria or extracellular fluorescent signals produced by these bacteria...culture flasks. In cases where the culture is irreplaceable or very expensive, there are some ways to rescue...cultures because you are not the first person to face this kind of problem. Educate others when you can...

...important of those is to always look closely at the trace file (or chromatogram) of the sequencing results...sequence (no Ns in sight). If you never looked at the trace you would be happy. But look closer, the overlapping... 2. You should see individual, sharp and evenly spaced peaks …like these…. 3. Expect to get 500-700 bases...you can purify the product either via the Sodium Acetate/isopropanol precipitation method or using a silica...

...or by project, if that works better for you) and place them on an easy to reach shelf. Make sure all notebooks... Post-its and write brief descriptions on each. Place the notes sticking up out of the top of the notebooks... notebooks (ELNs) have gained traction with pharmaceutical companies, academic labs have been slower to...find to where the binder was moved. You’ll have peace of mind knowing the information is backed up and...

...to choose a target sequence with a nearby protospacer-adjacent motif (PAM)? Cas9 is becoming an ever more... structure to interrogate the sequence isn’t in place yet.     Figure 4: S. pyogenes Cas9 in the...perfect guide RNA will do you no good if there is no adjacent PAM to anchor it. However, Cas9 only checks the...HNH domain, ensuring all binding partners are in place before cleavage can occur (Jiang & Doudna, 2017;...

...talk about Addgene’s internship opportunity taking place next summer.  I applied to Addgene and began the...first week at Addgene, I had to get used to the workspaces, as well as complete the lab safety and cybersecurity... clones because the bands were all in the wrong place. Except for one. My manager speculated that the ...safety, general work skills — such as using Google workspace — and got the chance to work on my very own research...

...best place for the biosensor to be expressed. Are you looking at an intracellular or extracellular molecule...calcium sensor CMV-mito-GEM-GECO1  and blue flame extracellular glutamate sensor pCMV(MinDis).iGluSnFR. Picking...

...special words about managing scientists because, let’s face it, we are special. Below are some special characteristics...characteristics of scientists in the work place as described by Sebastiano Massaro in his Nature Biotechnology...approached as peers rather than subordinates Need mental space and dislike intrusions (this actually varies, a ...scientists) Are highly mobile and can move to a new workplace if opportunities for learning and personal growth...

...science writing in magazines or newspapers or other places online, and consider how those stories are being...their blogs. 5)   Local science museums are a great place to find opportunities for science outreach. Here...institution; the news or communications office is a good place to start. It’s not unusual to find science writers...Mass Media Fellowship or maybe an internship at a place like Science News. Ask your institutional news office...

...genetic material next to the species-specific protospacer adjacent motif (PAM). Once the CRISPR/Cas complex...blockbuster known as the CRISPR (clustered regularly interspaced palindromic repeats) Cas (CRISPR associated genes...genes. These data highlight an interesting "arms race" between phages and prokaryotes; the ultimate victor...

...some marketing experience, with little emphasis placed on the marketing or business background of the ...focus on the list of conferences I presented at and placed them under headlines entitled “Presentation Experience...more than necessary for my interview. The simplest place I could think of to start was “Marketing for Dummies...outside of academia, sales and marketing can be great places to start. Depending on what type of career you ...

...remember that industry career paths are not the only place to develop “industry related skills”. Many career...self-driving cars, tissue engineering for organ replacement).  In addition to presenting your expert skills...Google Scholar account Sanitize your LinkedIn, Facebook profile, online presence (if you need to).  Immigration...for international candidates. Being able to “put a face to a name” will help a candidate get looked at and...investment banking and venture capital areas.  We place individuals into roles in areas including: research...