Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 13 results for: ACE

Showing: 1 - 13 of 13 results

  1. Chemogenetics Guide

    Type
    Guide
    ...Receptors Exclusively Activated by Designer Drugs. ACS Chem Neurosci. 6:476–484. PMID: 25587888 (Link opens...receptors, or GPCRs. The largest class of cell surface receptors, GPCRs are seven-pass transmembrane proteins...stimulating phospholipase C, which releases intracellular calcium stores. There are currently three Gq...and activate neuronal signaling by increasing intracellular cAMP concentrations. There is currently one ... was created by replacing the corresponding intracellular region of a turkey erythrocyte β-adrenergic ...pronounced SAMs). PSAMs are engineered α7 nicotinic acetylcholine receptor (nAChR) domains that respond to specific...

  2. CRISPR Guide

    Type
    Guide
    ...The target is present immediately adjacent to a P rotospacer A djacent M otif (PAM) The PAM sequence (NGG...codons that make up a gene PAM P rotospacer A djacent M otif; Sequence adjacent to the target sequence that...referred to as CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeat) technologies. Before...for Cas-binding and a user-defined ∼20-nucleotide spacer that defines the genomic target to be modified....through interactions between the gRNA scaffold and surface-exposed positively-charged grooves on Cas9. gRNA... an active, DNA-binding configuration, with the spacer region of the gRNA left free to interact with the...Cas9 will only cleave a given locus if the gRNA spacer sequence shares sufficient homology with the target...

  3. Antibody Guide

    Type
    Guide
    ...suspended in the original media or isolated and placed in a buffer. Antibody Structure Figure 1: Structure...of 4 IgA1; IgA2 IgD Coexpressed with IgM on the surface of most immature B cells; function is unknown Monomer...by a capture antibody (sandwich). Block unused surface-binding sites in the wells with a blocking protein...sandwich ELISA, a capture antibody, bound to a surface, is used to capture the protein of interest and...In IHC, tissue sections are assayed and the extracellular matrix remains intact. The signaling molecule...enzyme. In ICC, cells have most or all of their extracellular matrix removed. Again, the signaling molecule...cells. Cells, with labeled antibodies bound to surface receptors, are “flowed” through a flow cytometer...

  4. Adenovirus Guide

    Type
    Guide
    ...included between the ITRs is limited by the physical space available inside of the adenoviral capsid and directly...packaging cell line, which not only frees up more space for genetic cargo but also improves safety. In order...ITRs and the packaging signal, which is located adjacent to the left ITR, resulting in the possibility ...contain the viral gene E4, adding 2.7 kb of packaging space. However, these constructs must be transfected into...encapsidation signal located at the left arm of the genome adjacent to the left ITR that is required for viral genome...Icosahedral Having the shape of a polyhedron with 20 faces, each being an equilateral triangle. Immunogenicity...

  5. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...included between the ITRs is limited by the physical space available inside of the small AAV capsid and directly...information to a given region. This allows researchers to trace connections between neurons, map the brain, and ...Cells AAV Purification by Iodixanol Gradient Ultracentrifugation AAV Titration by ddPCR AAV Titration by qPCR... viral vectors for enhanced gene transfer . Pharmaceutical Research, 25 (3), 489–499. https://doi.org/...by stimulating elongation and stabilizing the displaced single-stranded DNA. VA in trans Adenoviral gene...Icosahedral Having the shape of a polyhedron with 20 faces, each being an equilateral triangle. Immunogenicity...

  6. Optogenetics Guide

    Type
    Guide
    ...better tissue penetrance, which may allow you to place the optic fiber outside of the brain, rendering ... activated by the light is controlled via the placement of the optic fiber. Using viral vectors for opsin... the toxin's local concentration near the cell surface, and enabling the ion channel to function. TULIPs...

  7. Molecular Biology Reference

    Type
    Guide
    ...mL 10 µg/mL *Note: Carbenicillin can be used in place of ampicillin. Preparing Antibiotics Create a stock...Adenine, Cytosine, Guanine and Uracil (U). Uracil replaces thymine in RNA molecules. Every 3 nucleotides ...

  8. Science Guides

    Type
    Guide
    ...Read More CRISPR Class 2 C lustered R egularly I nterspaced S hort P alindromic R epeat (CRISPR) systems,...

  10. Promoters

    Type
    Guide
    ...Promoters are about 100-1000 base pairs long and are adjacent and typically upstream (5’) of the sense or coding...

  11. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...plasmids, this region is removed in 5’ LTRs and replaced with a heterologous promoter (usually CMV or RSV...

  12. Plan Your Experiment

    Type
    Guide
    ...Edit References CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) is a powerful system...

  13. Lentiviral Vector Guide

    Type
    Guide
    ...plasmids, this region is often removed in 5’ LTRs and replaced with a heterologous promoter (CMV or RSV). 5' ...
Showing: 1 - 13 of 13 results