We narrowed to 835 results for: ANG

...trolls. People don’t like to connect with complete strangers as it borders on my first rule of networking--...a useful way to build or use a network. Total strangers can’t really help or refer you. Do not ask people... lot about requesting connections to complete strangers for informational interviews. Many have had success...game for connecting to others.  Pro tip: You can change the option for sending LinkedIn messages to Click...considered intrusive and it’s still connecting with a stranger so is highly discouraged. You should be using ...Turn off notifications to your network of profile changes. If you are working, and all of a sudden you start...

...all incoming plasmids was a big change for Addgene - we had used Sanger sequencing for quality control ...fall within a 10 fold range. Plasmids that don't meet the lower bound of this range are “failed” and removed.... Priority number one was to ensure that this changeover had no impact on our ability to provide requesting...Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F (2017) Genome-scale CRISPR-Cas9 knockout and transcriptional...

...resolve this issue, Shangqin Guo and her colleagues at Yale University fused a color-changing fluorescent timer...proteins system for Caenorhabditis elegans By Jennifer Tsang Fluorescent proteins (FPs) traditionally introduced...’s native locus, but introducing large genetic changes in C. elegans with CRISPR can be difficult since...synthesis and, after a few hours, a conformational change causes the protein to fluoresce red. H2B-FT-red...j.celrep.2020.107804 The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR plasmids...

...of [after undergrad] was to learn and speak the language of science. I didn’t come out of [undergrad] feeling...work,” she said. “School will always be there.” Changing your path! It’s okay! Our blog writer, Beth Kenkel...research and technical assistant, when her life changed––she had a daughter. Needing a career switch because...thought, ‘Let me pursue this, let me make a slight change so I can have flexibility and be up to date with...Bioinformatics it is.’” Though she misses the bench, the change gave her the balance she needed. It is incredibly...incredibly helpful to me to know that you can make big changes in your career path and still find comfort and ...

...•T-to-G•C base changes (Figure 1), but it can be challenging to make these base changes, especially in... protospacer, the editing capacity of the ABE8s ranged from 1.5x higher for positions A5-A7 to 3.2x higher...hemoglobin. Previous studies have shown that one base change in the promoter region of gamma hemoglobin (HGB1...effects. ABEs have been described to introduce base changes in cellular DNA and RNA dependent or independent...authors tested ABE8.17-m and found that one base change mutation (ABE8.17-m+V106W) (Gaudelli et al., 2020...

...:10.1126/science.aas9129   Zhang W, Yin J, Zhang-Ding Z, Xin C, Liu M, Wang Y, Ai C, Hu J. In-depth assessment...patient mutation or need to make a precise amino acid change, there is very little flexibility in the location...shown to be less effective at NGH PAMs, though (Zhang et al, & Walton et al). Additionally, SpG Cas9 with...NGAG - xCas9 NG Less effective at NGH PAMs (Zhang et al, 2021) NG Cas9 NG - SpG Cas9  NG  ...

...there were many changes to the  Board of Directors and the Executive Director changed twice. I had to ...liaise with the Executive Director. As members changed, I had to learn how to develop new working relationships... had different styles from their predecessors. Changes were implemented and I had to adapt accordingly...write on behalf of the Advocacy Committee would change format depending on the particular style and requirements...growing too and with that would come necessary changes. I also learnt to adapt my leadership style according...

...O'Neill, P. S., Villette, V., Bradley, J., Wang, Z., Jiang, D., Zhang, G., … Lin, M. Z. (2024). A fast and ... J., Luebbers, A., Green, E., Philibert, C. E., Zhang, H., Layne, M. D., Wu, J. C., & Garcia-Marcos, M...diseases (Diabetes & cancer). Image adapted from Wu and Wang 2024 under a CC-BY 4.0 license. Find more neuroscience... neuroscience antibodies here! Wu, Z., Wang, W. (2024). Anti-APP [6E10-A5] in Immunohistochemistry (Human...

... are sensitive to pH. Anything between orange and light orange usually indicates that the cells have grown... 37 °C and 5% CO2 and get agitated with sudden changes in temperature (especially if you have air conditioning... to recover after they are passaged. This could change their growth rates and morphology, rendering their...culture media contain the pH indicator phenol red (pH range 6.0 (yellow) - 8.0 (red)). Blood pH normally lies...numbers Keep an eye on the passage number. Cells change their growth rate and morphology at high passage...

...CFD scores (which range between 0 and 100) are then converted to a weight (which ranges between 0 and 1)...These values (ranging from -40 bp to 40 bp) are also converted to a weight (which ranges between 0 and...cut-to-insert distance) This gives each guide a score ranging between 0 and 1. The cutting frequency determination...specificity of a guide to the target. The CFD score ranges between 0 and 100 for each guide, with 100 being...

...prepping. Be sure to stay within the recommended range of the filter or you might inadvertently lower the... the culture medium. Improve the culture media Changing to a richer media or adding supplements can improve... low copy plasmids or slower growing strains, a change in media may be advantageous. Reduce the antibiotic...for high copy plasmids in standard growth strains range from 12-16 hours, but cultures with lower copy plasmids...oxygen for optimal growth, as insufficient gas exchange will prevent your cultures from reaching the desired...shaker speed is fast enough for sufficient gas exchange in the overnight culture. Generally, large cultures...

...players in the CRISPR field. Just a year after Feng Zhang’s lab identified Cas13a (C2c2) (Abudayyeh et al.,...end joining (NHEJ). Designing an RNA editor The Zhang lab envisioned a two-component RNA editor: a Cas13...adenosine to inosine in RNA, creating a functional A->G change. They fused ADAR deaminase domains (ADARDD) to ...introducing a C opposite the target A to be edited. This change specifies the edit to be made when multiple As ...Capabilities through RESCUE Two years later, the Zhang lab expanded the RNA editing toolkit to allow C ... their RESCUE (RNA Editing for Specific C-to-U Exchange) system (Abudayyeh et al., 2019). To avoid some...

... dilution should fall within the range of the assay for a wide range of titers. We usually dilute our ...determine if your titers are within the expected range. We have also found that generating the standard...virus. It is important to note that the dynamic range of the ddPCR is between 1 and 100,000 genome copies...per reaction. Since AAV titers tend to be in the range of 1012 to 1013 GC/mL, you must serially dilute .../s is recommended to ensure an even temperature change among all of the droplets, but you can go as low...

...preferred buffer. If that’s the case, a buffer exchange can be performed using a desalting column or an...western blot. This can be due to conformational changes in the antigen or lower detection sensitivity of...antibodies may impact their quality and efficacy (Wang et. al., 2018). Aggregation can arise from a shift.... PMID: 23908655; PMCID: PMC3725456. Schwarz H, Zhang Y, Zhan C, Malm M, Field R, Turner R, Sellick C,...j.ijbiomac.2015.03.027. Epub 2015 Mar 25. PMID: 25819219. Wang W, Roberts CJ. Protein aggregation - Mechanisms,...A. Pathak, Christopher J. Roberts, Prasad S. Sarangapani, Protein aggregation, particle formation, characterization...

...olds in a single evening. Grades K-5 cover a broad range of attention spans, skill levels, and STEM exposure...Chemistry activities from Quatr.us Foldscopes Steve Spangler Science The Fab Lab Middle school: supplementing... key concepts (Fig. 2). One thing I will likely change for next time is giving fewer examples and think...regulated. I did not try to sugarcoat the potential dangers of people using the technology in an unethical ...aspects of talking to 8th graders was the broad range of questions I received. I gave my presentation ...each, and each group went on its own wonderful tangents. Some groups asked about vaccines, others were...

...critical regions of plasmids deposited with us via Sanger sequencing, while more recently we have moved to...design, we were free to use multiple programming languages (Python, Perl, Java, R, etc.) in our pipeline.... to translate or convert programs to a uniform language.  The modern architecture of Nextflow included...Python scripts, queried our database with SQL or Django, and executed code in Jupyter notebooks using familiar...Since a Nextflow process can utilize any scripting language that can be executed on Linux, we created our ...through much trial and error to learn the scripting language, but our path forward was clear: to build one ...

...will always be that way. Things change and processes often have to change with them. If you can, set aside...concept and pare it down until you have something tangible and applicable to your situation.   Thinking Outside...creative when you don’t believe you have the power to change anything. You may not have the highest position...received, understand that people can be resistant to change. Ask your peers or your superiors to explain their...unworkable idea into a viable solution.  Anticipate Change Just because a process is working, doesn’t mean...

...molecular language shared by every living thing fit onto a single lecture slide. That shared language is the...to our Viral Vectors 101 series. Are broad host range plasmids shuttle vectors? While it’s technically... to say that shuttle vectors have a “broad host range,” these terms are typically used in different (though...(though overlapping) ways. Broad host range plasmids are those that can replicate in multiple kinds of...incredible that my cells “speak” the same molecular language as the tree outside my window! Whatever you use...

...increase gene expression at certain loci to create changes in phenotypes. For example, CRISPR activation has... constructs, however, Cas9 allows you to easily change the sequence targeted by the activator by simply...Voges M, Schäfer C, Spohn M, Stucka R, Hauber J, Lange UC (2016) Targeted HIV-1 Latency Reversal Using ... Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F (2014) Genome-scale transcriptional activation...doi.org/10.1016/j.cell.2014.09.039 Wong N, Liu W, Wang X (2015) WU-CRISPR: characteristics of functional...doi.org/10.1186/s13059-015-0784-0 Zetsche B, Volz SE, Zhang F (2015) A split-Cas9 architecture for inducible...

...This technique uses the trace file generated in Sanger sequencing and a simple algorithm to quantify editing... to amplify the target region of interest. Then Sanger sequence the PCR product of both an unedited population...enzyme screening is sensitive to single base pair changes, but the edit must occur at an existing restriction...simultaneously validate edits and look for off-target changes. This method requires a set of control cells to...option, you can still detect off-target effects with Sanger sequencing, provided you have some idea of where...where off target editing may occur, allowing you to Sanger sequence a manageable number of the higher probability...