...(Shaner et al, 2008). Obligate dimers (such as dTomato) that promote interactions between the FPs interfere...CytERM-mKOkappa, CytERM-mTurquoise2, and CytERM-dTomato. When using and interpreting the results of the...proteins. Fusions of CytERM with mTurquoise2 and dTomato, which are established monomeric and dimerizing...

... Laboratory management software is not a requisite for a functioning lab, but it is for a scaleable lab...about what steps are most likely to fail. Our laboratory manager would definitely be interested to see...this case, the physical considerations of the laboratory make object composition a smarter choice than...

...Library, deposited by Carrie Bhang, a research investigator in the In Vivo Pharmacology group at Novartis...ClonTracer Since starting her new role as an investigator in the In Vivo Pharmacology group at Novartis...Addgene community! Carrie Bhang is a research investigator in In Vivo Pharmacology at Novartis Oncology...

...), Antibodies: a Laboratory Manual, 2nd Edition. 2104. Cold Spring Harbor Laboratory Publications, New...production and subsequent purification.  Academic laboratories and companies have already begun creating plasmids...

...They play important roles in many cellular and regulatory processes, but much of their functionality is...effects can be minimized by disrupting lncRNA regulatory elements that are crucial for transcription and..., on the other hand, recruits transcriptional activators such as VP64, SAM, or SunTag to specific ncRNA...

...with a terminator. A simple TU could be comprised of just three parts: promoter, gene, and terminator.   ...this category also includes promoters, UTRs, terminators, etc. Once selected, individual units can be ...

...the field. 3. Highlight your network and your collaborators It’s a good idea to move around to different...Networking for Scientists Series 4. Become a science communicator More and more fellowship applications are asking...on your grant application to your peers and collaborators can also help you get some critical insight ...

...important supporting roles. Enhancers are cis regulatory DNA sequences that, when bound by transcription...CAPTURE (CRISPR Affinity Purification in situ of Regulatory Elements) method. The original CAPTURE method...2020). CRISPR Affinity Purification in situ of Regulatory Elements: The original CAPTURE method The original...

...multiple cell types of the same lineage. Blood (hematopoietic) stem cells are a good example of multipotent...numbers or replenish dying cells. For example, hematopoietic stem cells found in the bone marrow only differentiate...three germ layers, form embryoid bodies, and form teratomas [11]. iPSCs also possess similar morphologies,...

...air conditioning on) so leave them in a sterile incubator. This also means that any medium or washing buffer...entering the hood and when putting them into the incubator). It also helps to get familiar with the morphology...results. Most cell culture media contain the pH indicator phenol red (pH range 6.0 (yellow) - 8.0 (red))...

...  Is this department/laboratory collaborative with other departments/laboratories? How long is the average...by meeting with professors - a/k/a Principle Investigators (PIs) - whose labs does research that aligns...

...localization, dynamics, and behavior of a POI. As an investigator, you want a fusion tag to be inert, to not produce...has gone into making FPs monomeric, yet many investigators remain ignorant of FP dimerization. Equally ...Einstein College of Medicine in the Department of Anatomy and Structural Biology. His interests include the...

...expressing eGFP under the human ubiquitin C promoter. Anatomical abbreviations: Gr= Gracile tract, Cu= Cuneate...  The first successful attempt to render fixed anatomical preparations transparent was done by Walter Spalteholtz...protein (GFP) (6).   The Dodt and Frank Bradke laboratories later developed the 3DISCO (3-dimensional imaging...

...genetically encoded calcium indicators (e.g. GCaMP). Others include: pHluorin & pHTomato (pH sensors), HyPer ...fluorescent intensity upon pH changes (e.g. pHluorin, pHTomato). Keep this list handy as you plan your next ...

...available by both the Jacob Corn and Alex Schier laboratories. Delivering Cas9-gRNA ribonucleoproteins  An...and purification, which will require additional laboratory reagents, equipment, and expertise. Final thoughts...production of the Cas9 protein and a gRNA in your laboratory, once these have been acquired, genomic editing...

...communication should be fun and engaging for the communicator as well as the audience, so pick a mode of communication...Otherwise, you’re going to drop your project like a hot potato or burn yourself out trying to slog through unappealing... Low barrier to entry for both audience and communicators. Well-developed content creation platforms.  ...

...and debris. Affinity chromatography is a popular choice for this. Chromatography columns containing appropriate...using a desalting column or an ultrafiltration concentrator. As the name suggests, you may also concentrate...

...you possess because any potential employers, collaborators, or co-workers want to know that you are a well-rounded...situation? Maybe you demonstrated empathy for a collaborator or co-worker and showed that you really understood...Resources Cross team communication  External collaborators For further advice on highlighting your transferable...

...pictures (see figure 4). It also enables single-cell anatomical study and neuronal tracing, aka determining where...under the control of an inducer, i.e. tet-off transactivator (tTA), is injected at a high dose to maximize...An AAV expressing the inducer (i.e. tet-off transactivator) is co-injected. The dose of this AAV can be...

...do both, Addgene depositor Didier Trono and collaborators turned to the lentivirus HIV-1, well-known to...precursor protein), Pol (polymerase), Tat (viral transactivator for transcriptional activation from the 5’ ...trials for other diseases, including the common hematopoietic disorders sickle cell anemia and beta-thalassemia...