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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 709 results for: BASE;

Showing: 201 - 220 of 709 results

  1. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...School, he developed the first lentiviral microRNA-based system for polymerase II-regulated RNA interference...virology,” he says. He was working in an HIV lab and based on that experience, soon directed himself to finding...months, the lab began a project to develop an AAV-based COVID-19 vaccine.  Vandenberghe’s work with AAVs...

  2. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...from some labs have developed fluorescent-protein based DA sensors (Figure 2). The dLight1 dopamine sensor... dLight1 sensor is a single fluorescent protein-based sensor. This sensor was engineered from a GFP variant...sensor Yulong Li’s lab has created the GRABDA sensor based on a similar strategy: a GFP variant bound to a ...

  3. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ...polymerase, the construction of specific sequences for base pair overhangs, insert and vector purification, ...specific to the insert with an additional 15-17 bases that are complementary to the overlapping vector...introduced into my inserts. However, with any PCR-based cloning technique there is a chance of introducing...

  4. 8 Great Depositor Research Stories

    Type
    Blog Post
    ...tells us about a new tool for efficient multiplex base editing and multiplex prime editing (requiring minimal...dCas9 (with no modifications!) as a CRISPR/dCas9-based tool for DNA demethylation. AAV Q&A with Tim Miles...

  5. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Flag for bacterial expression pFA6a vectors - PCR-based C-terminal tagging in S. cerevisiae pCS2FLAG - N...expression pnCasPA-BEC - A cytidine deaminase-mediated base-editing plasmid in Pseudomonas species Vectors for...agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector GFP Varies pMKO.1 GFP...

  7. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...short ncRNAs, such as microRNAs that are around 20 base pairs long. Using a single guide RNA (sgRNA) to ...Figure 1: A schematic illustration of CRISPR/Cas9-based approaches to study ncRNAs. (A) Transcription start...your experiments, there are plenty of other CRISPR-based options that can target ncRNAs.  CRISPRi and CRISPRa...10.1126/science.1225829  Hazan J, Bester AC. CRISPR-Based Approaches for the High-Throughput Characterization...

  8. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...American, Africa, or South Asian) used in the screens based on the cells’ germline genetic variations. They ...generating genetic dependency data with an ancestry-based bias.   The correction process does not stop there...genome-wide screens, introducing bias into all work based on these and any other CRISPR screens using the ...variation contributes to false negatives in CRISPR-based experiments with varying burden across ancestries...Cancer Dependency Map (DepMap), a cancer dependency database used to make this information accessible to the...

  9. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...therapeutic potential. Note: constructs discussed are based on Adenovirus type 5. Three generations of adenoviral...delivery: Potential applications for gene and cell-based therapies in the new era of personalized medicine...formation assay on A549 cells . Alternatively, antibody-based assays are commercially available for faster detection...

  10. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...packaging systems have been developed over the years based on HIV-1; however, the 1st-generation vectors are...defines 2nd- or 3rd-generation transfer vectors based on whether or not they have a chimeric 5'LTR. Second-generation...? What percent of the vector components are HIV-based? A7: No single plasmid contains all the components...insertional mutagenesis. These risks are largely based upon the vector system used and the transgene insert...

  11. CRISPR Plasmids - RNA Targeting

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    Collection
    ...Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation ...

  14. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    ...and jargon Hemingway Editor  This is a free web-based tool for detecting the readability of your writing...or dissertations. Phrases are categorized either based on the section of a manuscript they might be found...to work with collaborators. Zotero is a desktop based program that’s compatible with Mac, Windows, and...directly import papers from web pages and academic databases.  Downloading Mendeley Cite lets you cite papers...

  15. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...While there are more and more molecular biology-based activities making their way into high school classrooms...BioBits ExplorerTM and BioBits BrightTM, biology kits based on freeze-dried, cell-free (FD-CF) components. Making...and is interested in developing synthetic biology based tools for science outreach and advocacy. You can...

  16. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...plexWell™ Library Preparation Technology for NGS-based sequencing and confirmation of Addgene’s large and...plexWell™ workflow also offers a highly efficient NGS-based alternative to Sanger sequencing because the sample... To date, this has resulted in high quality NGS-based assemblies for over 3000 plasmids in the Addgene...

  17. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...This version of the spike protein was developed based on 100 structure-guided spike designs and has improved...already developed nucleic acid detections tools based on Cas12a and Cas13  which could be repurposed for...JA, Finkelstein IJ, McLellan JS (2020) Structure-based design of prefusion-stabilized SARS-CoV-2 spikes... CM (2020) Open Sharing During COVID-19: CRISPR-Based Detection Tools. The CRISPR Journal 3:142–145. https...

  18. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...libraries utilize optimized sgRNAs that were designed based on the rules identified in Doench et al., 2014 to...assess library performance in viability screens. Based on these metrics the Brunello library was more effective...selected sgRNAs in this optimal window and ranked them based on their optimized sgRNA rules and potential off-target...Dolcetto library, sgRNAs were selected and ranked based on the previously established sgRNA rules and  potential...

  19. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...potential candidate genes that fell into 3 categories based on the architecture of the CRISPR protein in question...LshCas13a, on the other hand, uses only a short ~24 base crRNA that interacts with the Cas13a molecule through...spacer sequence and consists of a single A, U, or C base pair. In bacteria, once Cas13a has recognized and... the new development of Cas13b as a tool for RNA base editing,, and we are excited to see this group of...

  20. CRISPR Antimicrobials

    Type
    Blog Post
    ...developed sequence-specific, antimicrobial, plasmid-based, CRISPR systems (Find the Citorik Plasmids here)...encoded quinolone resistance, mediated by a single base pair change in DNA gyrase, CRISPR phagemids were...crRNAs) targeting either genomic (blue) or plasmid-based sequences (red). Treating a mixed population, represented... al. recently engineered synthetic phages, each based on the well-studied T7 phage, to target various ...
Showing: 201 - 220 of 709 results