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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15 results for: BASE;

Showing: 1 - 15 of 15 results

  1. CRISPR Guide

    Type
    Guide
    ... other base. In principle, the ability to induce base transversions means that any base-to-base conversion...handful of bases, or even a single base. These small precision edits can be done via HDR, base editing, ...main types of base edits: Base transitions - edit to same classification of nucleotide base (purine-to-purine...first classes of base editors were cytosine base editors (purine-to-purine) and adenine base editors (pyrimidine-to-pyrimidine...Overview of base editing More recently, researchers have developed base editors that can catalyze base transversions...Cas13a-based molecular detection platform. Based on this non-specific cleavage, a Cas13a-based molecular...Tagging CRISPR Base Editing To overcome low HDR efficiency, researchers have developed base editors to specifically...

  2. Plan Your Experiment

    Type
    Guide
    ...knockout Cut Nick Base Edit Generate specific base pair edits without double-strand breaks Base editor (dCas9...Cas9 nickase fusions with different base editing proteins) Many base editors fused to Cas9 exert their ...flexible PAM sites . Base Editing : Target the site of your desired edit. Many base editors exert their...confirm your edit using DNA-based methods like sequencing or digital PCR , RNA-based methods like qPCR , and...different types of edits, including point mutations, base changes, and large insertions or deletions. For ... very narrow window proximal to the PAM sequence Base Edit Prime Edit Generate targeted insertions, deletions...transcriptase (RT)) Can edit within a wider window than most base editors Editing efficiency can be low, but generally...

  3. Cloning

    Type
    Guide
    ...combining DNA fragments over 200 base pairs. Anything shorter than 200 base pairs has the potential to be..., add back the free base, and become stuck at this point (with no other free bases to add). Complementary...custom ordered DNA oligos of 60-80 base pairs in length, with 30-40 base pairs of homology to the ends of...Enzyme Cloning Restriction enzyme (endonuclease) based molecular cloning is the "classic" cloning method...specific target sequences, that range from 4 to 13 base pairs, and produce predictable resulting ends in...Gateway Cloning ) Gateway® cloning is a recombination based cloning method. The benefit of Gateway® is that ... from Plasmid 101: TOPO Cloning ) Toposiomerase based cloning, often called TOPO® cloning or TA cloning...

  4. Molecular Biology Reference

    Type
    Guide
    ... made up of 4 bases, adenine, thymine , cytosine, and guanine. The order of these bases makes up the genetic...is a double helix, where a base on one strand pairs with a particular base on the other, complementary...modified bases and a microscope captures the fluorescent color that is emitted each time a base is added...Sanger however, these modified bases can be converted back to a regular base and thus do not halt the reaction...code in addition to ambiguous bases and common epitope tags. Ambiguous bases are included in a DNA sequence...plasmid. DNA and RNA Single Letter Code: Primary bases Nucleobase A Adenine C Cytosine G Guanine T Thymine U... U Uracil Single Letter Code: Ambiguous bases Nucleobase B C, G, or T D A, G, or T H A, C, or T K G or...

  5. Chemogenetics Guide

    Type
    Guide
    ...two Gi DREADDs based on human muscarinic receptors, hM2Di and hM4Di, and a Gi DREADD based on the human ...Receptors: RASSLs The first chemogenetic receptors were based on G-protein coupled receptors, or GPCRs. The largest... Activated Solely by Synthetic Ligands (RASSLs) based on κ-opiod receptors (Coward et al. 1998). These...Activated by Designer Drugs (DREADDs), like RASSLs, are based on engineered G-protein coupled receptors, but unlike...calcium stores. There are currently three Gq DREADDs based on human muscarinic receptors: hM1Dq, hM3Dq, and...of G proteins. This DREADD, termed Rq(R165L), is based on the human M3 muscarinic receptor and has not ... properties. DREADD Ligands Muscarinic-receptor based DREADDs were engineered to respond to nM concentrations...

  6. Antibody Guide

    Type
    Guide
    ...increase flexibility or specificity in antibody-based methods. Antibodies can recognize and bind other...produce the same antibody clone, or from plasmid-based expression. Monoclonal antibodies are necessary .... The complexity of this can vary significantly based on the antibody and conjugate chosen. Signal Amplification...assay. Overview of Antibody Applications Antibody-based applications can be generally classed into four ...Enzyme-linked immunosorbent assay (ELISA) is a plate-based assay that uses standard curves to quantify exact...strategy. Figure 5: Immunoprecipitation using bead-based antibody capture to isolate specific proteins from... cells are then slotted in a specific direction based on the emission(s) read by the machine. Controls...

  7. Optogenetics Guide

    Type
    Guide
    ...activated. Optogenetics tools can be broadly classified based on their functions into two groups: Actuators are...Phytochrome, cryptochrome, and LOV (light oxygen voltage)-based systems have been used in many experimental contexts...Blue-light–mediated induction of protein interactions based on Arabidopsis thaliana cryptochrome 2 and CIB1 ...to control calcium channels ~488 LOV domains LOV-based Light-oxygen-voltage-sensing (LOV) domains are protein...LINUS light-inducible nuclear localization signal based on the LOV2 domain of Avena sativa phototropin 1...blue light. 470 Fluorescent Protein domains Dronpa based Light-inducible Dronpa mutant domains that associate...profile 282 References Optogenetic Switches from OptoBase, W. Weber Lab Edward Boyden's Lab: Synthetic Neurobiology...

  8. Addgene's eBook Collection

    Type
    Guide
    ...Plasmids 101 September 2023 | Fourth Edition About Based on our popular Plasmids 101 blog series, this eBook...practical introductions and helpful tips for antibody-based applications, from flow to IHC! Close About Overlay...

  9. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...produce AAV vectors. Additional production systems based on other human cell lines, such as HeLa and A549...low risk of insertional mutagenesis. Several AAV-based gene therapies have been approved by the US Food...muscular dystrophy. Many clinical trials of AAV-based gene therapies are underway and are promising for...one of the most common methods for in vivo CRISPR-based genome editing. Packaging the Cas9 from Streptococcus...the rAAV capsid, such as Cas9-derived plasmids and base/prime editors, is to create split AAVs. Here, a ...genes provide a helper function for transfection-based adeno-associated virus vector production . Journal...

  10. Adenovirus Guide

    Type
    Guide
    ...genetic cargo into cells. Most rAdV vectors are based on adenovirus type 5 (Ad5) and have been produced... adenoviral vector production system. Figure is based on the use of pAdEasy-1. Created with BioRender....be very useful for designing and producing rAdV-based vaccines. Adenoviral vectors are excellent vectors...delivery: potential applications for gene and cell-based therapies in the new era of personalized medicine...

  11. Promoters

    Type
    Guide
    ... expressed. Summary Promoters are about 100-1000 base pairs long and are adjacent and typically upstream...The proximal promoter is found approximately 250 base pairs upstream from the TSS and it is the site where...regulatory elements such as enhancers several kilobases away from the TSS. Eukaryotic promoters are so...

  12. Science Guides

    Type
    Guide
    ...highly specific binding to other proteins. Antibody-based applications, which often use antibodies conjugated...

  13. Lentiviral Vector Guide

    Type
    Guide
    ...including knockout, inhibition/activation, and prime and base editing. Browse Addgene's collection of CRISPR libraries...virus. The potential for oncogenesis is largely based on whether or not the specific insert contained ... Swaminathan, T. A., & Braun, S. E. (2021). HIV-based lentiviral vectors: Origin and sequence differences...

  14. Sequencing Primers

    Type
    Guide
    ...plasmids in our repository, we've chosen primers based on the plasmid backbone and insert. To identify ...

  15. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...virus. The potential for oncogenesis is largely based on whether or not the specific insert contained ...
Showing: 1 - 15 of 15 results