We narrowed to 700 results for: BASE;

...Resources Two classes of base editors — cytosine base editors (CBEs) and adenine base editors (ABEs) — can... CRISPR Base Edit CRISPR Plasmids: Base Edit Mammalian... to generate single base pair edits without double stranded breaks. Cytosine base editors are created ...cytosine base editors, the evolved TadA domain is fused to a Cas9 protein to create the adenine base editor...Overview of CRISPR base editing. Browse, sort, or search the tables below for CRISPR base editing plasmids...precisely editing DNA bases without inducing a DNA break. CRISPR...Cas9 (dCas9) to a cytidine deaminase like APOBEC. Base editors are targeted to a specific locus by a gRNA...

...knockout Cut Nick Base Edit Generate specific base pair edits without double-strand breaks Base editor (dCas9...Cas9 nickase fusions with different base editing proteins) Many base editors fused to Cas9 exert their ...flexible PAM sites . Base Editing : Target the site of your desired edit. Many base editors exert their...confirm your edit using DNA-based methods like sequencing or digital PCR , RNA-based methods like qPCR , and...different types of edits, including point mutations, base changes, and large insertions or deletions. For ... very narrow window proximal to the PAM sequence Base Edit Prime Edit Generate targeted insertions, deletions...transcriptase (RT)) Can edit within a wider window than most base editors Editing efficiency can be low, but generally...

... other base. In principle, the ability to induce base transversions means that any base-to-base conversion...handful of bases, or even a single base. These small precision edits can be done via HDR, base editing, ...main types of base edits: Base transitions — edit to same classification of nucleotide base (purine-to-purine...first classes of base editors were cytosine base editors (purine-to-purine) and adenine base editors (pyrimidine-to-pyrimidine...Overview of base editing. More recently, researchers have developed base editors that can catalyze base transversions...Cas13a-based molecular detection platform. Based on this non-specific cleavage, a Cas13a-based molecular...Tagging CRISPR Base Editing To overcome low HDR efficiency, researchers have developed base editors to specifically...

...ADP based on tetramethylrhodamine-labeled ParM A fluorescent, reagentless biosensor for ADP based on tetramethylrhodamine-labeled...fluorescent protein-based cAMP indicator (Pink Flamindo) Red fluorescent protein-based cAMP indicator applicable...(GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-Based cGMP ...peroxiredoxin-based probes Real-time monitoring of basal H 2 O 2 levels with peroxiredoxin-based probes. Nat...FUCCI: fluorescent ubiquitination-based cell cycle indicators RNA-based aptamer sensors for metabolites ...GENIE Project Calcium jGCaMP7 High-performance GFP-based calcium indicators (Constitutive or Cre-dependent...924. Yubin Zhou Calcium Twitch calcium indicator, based on C-terminal domain of troponin C Griesbeck Lab...

...genetic information into a specific DNA site. Base Edit Base editors precisely edit DNA without promoting...your first CRISPR experiment. CRISPR 101 eBook : Based on our popular CRISPR 101 blog series, we've organized...Editing Cas13 enzymes fused to deaminases can edit bases in RNA. Other Applications Screen CRISPR libraries...Vectors Select an empty vector for your experiment based on a variety of factors. Browse Plasmids by Model...

...genes and watermelon mutant clones for your course-based undergraduate research experience (CURE) projects...CUREs Community (MCC) Collection Blog post: Course-Based Undergraduate Research Experiences (CUREs) (Link...in a new window) develops protein-centric Course-based Undergraduate Research Experiences (CUREs) centered...the search bar or sort buttons to find plasmids based on: Organism or species (e.g., human, watermelon...36206327 (Link opens in a new window) Length of course-based undergraduate research experiences (CURE) impacts...

... zinc finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make the zebrafish a stalwart research...expression plasmids - Keith Joung Lab CRISPR/Cas9-based conditional mutagenesis in zebrafish - Wenbiao Chen...Disruption in Zebrafish - Leonard Zon Lab Programmable base editing of zebrafish genome using a modified CRISPR-Cas9...zebrafish. NTR 2.0 - Jeff Mumm Lab. NTR 2.0/MTZ-based cell ablation system that allows sustained cell ... Kit - Hai-Ning Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS), bearing either...Zebrafish International Resource Center (ZIRC) - A US-based repository for zebrafish strains and materials and...Zebrafish Resource Center (CZRC) - A resource center based in China focused on collecting existing zebrafish...

... not rely on NHEJ or HDR: Base editors : Base editors directly convert bases without creating a double-strand...editing. The first base editors converted cytidine to thymidine; newly engineering base editors convert ... classification defines 5 types and 16 subtypes based on shared characteristics and evolutionary similarity...similarity. These are grouped into two large classes based on the structure of the effector complex that cleaves...2013, Cong et al. and Mali et al. described CRISPR-based genome editing in mammalian cell culture. Five years...Côté J, Doyon Y. 2015. A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human..., Liu DR. 2016. Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage...

... Addgene's collection: HIV-based lentiviral particles Murine leukemia-based retroviral particles Vesicular... Express firefly luciferase. Used in lentivirus-based SARS-CoV-2 related pseudovirus assay. David Nemazee...pCMV-FLuc Express firefly luciferase. Used in MLV-based SARS-CoV-2 pseudovirus assay. David Nemazee 161030...

...opens in a new window) — Database of gene expression profiles in A. thaliana based on RNA-seq analysis. Figshare...rapidly assembling plant transformation constructs, based on the Golden Gate method. Golden Gate (GreenGate...Plasmids for gene editing using Cas nucleases and base editors in monocots and dicots. CRISPR Vladimir ... in plants. JoinTRV , an improved vector system based on tobacco rattle virus (TRV) that simplifies gene...fluorescent proteins. A set of fluorescent protein-based markers expressed from constitutive and arbuscular...Plant Genome Using CRISPR/Cas9 CRISPR-mediated Plant Base Editors RNA Interference in Plant Biology: New Tools...(Link opens in a new window) — TAIR includes a database of genetic and molecular biology data for A. thaliana...

...Reactivity is based on validation data from Addgene or trusted partner labs or is predicted based on sequence...Addgene supplies a list of recommended applications based on our in-house testing and data provided by trusted...

... information on Addgene's collection of plasmid-based antibodies, including monoclonal antibodies, scFvs...super-resolution microscopy. Two commonly used plasmid-based antibody fragments are scFvs and single-domain antibodies... that can be used to create and express plasmid-based antibodies. Description Article PI One-step assembly.... 2015 Jan 2;456(1):527-33. Gavin Wright pET-30-based vector dedicated to efficient scFv expression, which...

...Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation ...Myers labs have deposited plasmids for a CRISPR-based system to add a tag (currently using FLAG) to endogenous... A vector containing Cas9 and a validated gRNA, based on the Zhang lab's PX458 . Multiple gRNA plasmids... deposited the PITCh system plasmids for CRISPR-based knock-in of EGFP-2A-PuroR cassette to the C-terminus...two components: An all-in-one CRISPR/Cas9 vector based on the Zhang lab's PX330 , simultaneously targeting... has developed a simple and scalable CRISPR/Cas-based method to tag endogenous proteins by using donor...

... antibody plasmids to create or express plasmid-based antibodies. Most antibodies in the collection have...developed using a platform (Link opens in a new window) based on yeast display technology (Link opens in a new...plasmids can be used to create or express plasmid-based antibodies. ID Plasmid Description Return to top...

... CRISPR Plants CRISPR Plasmids: Plants Activate Base Edit Cut Interfere Nick Prime Edit Resources The...Promoter Selectable Marker PI Publication Return to top Base Edit ID Plasmid Gene/Insert Promoter Selectable ...

... CRISPR Yeast CRISPR Plasmids: Yeast Activate Base Edit Cut Interfere Nick Purify Resources The following...Promoter Selectable Marker PI Publication Return to top Base Edit ID Plasmid Gene/Insert Promoter Selectable ...

...Biolabs offers non-toxic protein labeling systems based on SNAP-, CLIP-, ACP-, and MCP-tags for visualization...-tag, and GFP . Technology SNAP-tag SNAP-tag is based on the human O6-alkylguanine-DNA-alkyltransferase...MCP tags ACP- and MCP-tags are small protein tags based on the acyl carrier protein. Substrates are derivatives...

...CRISPR Bacteria CRISPR Plasmids: Bacteria Activate Base Edit Cut Interfere Nick Prime Edit Purify RNA Targeting...Gene/Insert Promoter PI Publication Return to top Base Edit ID Plasmid Promoter PI Publication Return to...

...off-target evaluation for base edits using adenine (ABE) and cytosine (CBE) base editors. Includes a wide...e.g. Cas9, Cas12a) and noncleaving nucleases (e.g. base editing). Developed by the Luca Pinello Lab (Link...2019) (Link opens in a new window) . MAGeCK Model-based Analysis of Genome-wide CRISPR/Cas9 Knockout (MAGeCK...window) A webtool that uses deep learning models for base editor gRNA design and efficiency prediction. Developed...Juan L. Mateo (Link opens in a new window) Labs. ACEofBASEs (Link opens in a new window) Identification of...supports a wide selection of organisms. From the Database Center for Life Science (DBCLS) in Japan. Developed...

...Expression CRISPR Plasmids: Mammalian Expression Activate Base Edit Cut Epigenetics Interfere Nick Prime Edit Purify...Promoter Selectable Marker PI Publication Return to top Base Edit ID Plasmid Gene/Insert Promoter Selectable ...