We narrowed to 253 results for: CIL

1. MXS Chaining

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   Blog Post

   ... 3. The goal of high throughput cloning is to facilitate the construction of plasmids with many components...

2. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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   Blog Post

   ... research has shown that a Cas9 protein from Geobacillus stearothermophilus is more stable in human plasma...

3. CRISPR Antimicrobials

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   Blog Post

   ...opportunistic infection like for organisms like C. difficile. In 2014, Citorik et al. and Bikard et al. developed...

4. A Career in Grants: How To Become a Grant Professional

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   Blog Post

   ...national government offices, schools, healthcare facilities and with private businesses. How do you get the...

5. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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   Blog Post

   ...deficiencies found in the wildtype protein and facilitating its widespread use in research. Many other mutations...

6. Negotiating Work and Life: How to Find the Joy

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   Blog Post

   ... reminded her doctor that she was allergic to penicillin – I am much less worried knowing that she can...

7. Fluorescence Titering Assay

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   Protocol

   ...vial of cells after 20–30 passages. Do not add penicillin/streptomycin to the media, as it can affect viability...

8. Bioinformatics at Addgene

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   Blog Post

   ...we have moved to full plasmid sequencing at our facility using Illumina MiSeq Next Generation Sequencing...

9. CRISPR-based Genome Editing Systems For Editing in E. coli

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   Blog Post

   ...strain library with different gene dosages to facilitate swift optimization of strains. Multiple induction...

10. Hot Plasmids - August 2020

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    Blog Post

    ...expression. This includes a CRISPRi-based synthetic oscillator, bistable network, stripe pattern-forming incoherent...

11. AAV Titers: Where Do They Come From And What Do They Mean?

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    Blog Post

    ... Addgene and other viral vector manufacturing facilities report the physical titer of a viral solution...

12. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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    Blog Post

    ...model systems, and their resistance (so far) to facile CRISPR/Cas9-mediated insertion of foreign DNA. ...

13. 5 Reasons to Use Reddit for Science Communication

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    Blog Post

    ...r/Science has a great team of moderators who facilitated scheduling, filtered out inflammatory questions...

14. Important Considerations in Optogenetics Behavioral Experiments

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    Blog Post

    ...transmission from the amygdala to nucleus accumbens facilitates reward seeking." Nature 475.7356 (2011): 377-...

15. Plasmids 101: E. coli Strains for Protein Expression

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    Blog Post

    ...thioredoxin reductase and glutathione reductase to faciliate proper folding; may increase multimer formation...

16. Data Freedom: The Expansion of Data Sharing in Research Publications

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    Blog Post

    ...reasonable, sensitive to the data type and made as facile for both the author and the reader as possible....

17. Antibodies 101: Introduction to Gating in Flow Cytometry

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    Blog Post

    ...if you have access to a professional flow core facility, it’s always good to ask around for advice before...

18. Antibodies 101: Single Chain Fragment Variables (scFvs)

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    Blog Post

    ...intracellular T cell receptor portion of the CAR facilitates signal transduction and release of cytotoxic ...

19. Five Popular Model Organisms, Part 2

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    Blog Post

    ...research is The National Xenopus Resource, a breeding facility for X. laevis and X. tropicalis, the two most ...

20. Replacing Paper: Tips for Choosing an Electronic lab Notebook

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    Blog Post

    ...with them including reports and protocols. To facilitate this, some ELNs allow you to invite people and...