We narrowed to 253 results for: CIL

1. Fluorescence Titering Assay

   Type

   Protocol

   ...vial of cells after 20–30 passages. Do not add penicillin/streptomycin to the media, as it can affect viability...

2. CRISPR 101: Ribonucleoprotein (RNP) Delivery

   Type

   Blog Post

   ... research has shown that a Cas9 protein from Geobacillus stearothermophilus is more stable in human plasma...

3. MXS Chaining

   Type

   Blog Post

   ... 3. The goal of high throughput cloning is to facilitate the construction of plasmids with many components...

4. CRISPR 101: Making a Knock-In Cell Line

   Type

   Blog Post

   ...carefully (Lin et al., eLife)! Use a Cas that facilitates HR What if promoting HR was as simple as swapping...

5. A Guide to Starting Your Own Journal Club

   Type

   Blog Post

   ...level of critique and detail? Who is/are the facilitator(s) of the journal club? Where will the journal...

6. CRISPR Antimicrobials

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   Blog Post

   ...opportunistic infection like for organisms like C. difficile. In 2014, Citorik et al. and Bikard et al. developed...

7. A Career in Grants: How To Become a Grant Professional

   Type

   Blog Post

   ...national government offices, schools, healthcare facilities and with private businesses. How do you get the...

8. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

   Type

   Blog Post

   ...deficiencies found in the wildtype protein and facilitating its widespread use in research. Many other mutations...

9. Negotiating Work and Life: How to Find the Joy

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   Blog Post

   ... reminded her doctor that she was allergic to penicillin – I am much less worried knowing that she can...

10. AAV Titers: Where Do They Come From And What Do They Mean?

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    Blog Post

    ... Addgene and other viral vector manufacturing facilities report the physical titer of a viral solution...

11. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type

    Blog Post

    ...model systems, and their resistance (so far) to facile CRISPR/Cas9-mediated insertion of foreign DNA. ...

12. Hot Plasmids - August 2020

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    Blog Post

    ...expression. This includes a CRISPRi-based synthetic oscillator, bistable network, stripe pattern-forming incoherent...

13. Bioinformatics at Addgene

    Type

    Blog Post

    ...we have moved to full plasmid sequencing at our facility using Illumina MiSeq Next Generation Sequencing...

14. CRISPR-based Genome Editing Systems For Editing in E. coli

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    Blog Post

    ...strain library with different gene dosages to facilitate swift optimization of strains. Multiple induction...

15. 5 Reasons to Use Reddit for Science Communication

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    Blog Post

    ...r/Science has a great team of moderators who facilitated scheduling, filtered out inflammatory questions...

16. Important Considerations in Optogenetics Behavioral Experiments

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    Blog Post

    ...transmission from the amygdala to nucleus accumbens facilitates reward seeking." Nature 475.7356 (2011): 377-...

17. Plasmids 101: E. coli Strains for Protein Expression

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    Blog Post

    ...thioredoxin reductase and glutathione reductase to faciliate proper folding; may increase multimer formation...

18. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type

    Blog Post

    ...reasonable, sensitive to the data type and made as facile for both the author and the reader as possible....

19. Colony Formation Titering Assay

    Type

    Protocol

    ...vial of cells after 20–30 passages. Do not add penicillin/streptomycin to the media, as it can affect viability...

20. Fluorescent Proteins 101: When GFP lets you down

    Type

    Blog Post

    ...Adjobo-Hermans, 2011). Structural information facilitates the choice of the proper site, although it still...