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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 960 results for: Cre

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Showing: 201 - 220 of 960 results

  1. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...temperatures, but you may need to increase your primer length and increase the Tm if you are trying to clone...Bacterial Transformation Summary PCR based cloning is incredibly versatile and allows for nearly any piece of ... 2 ligations for each plasmid you are trying to create, with varying ratios of recipient plasmid to insert...

  2. Protocol - How to Streak a Plate

    Type
    Protocol
    ...of the plate, as shown in the diagram above, to create streak #1. Pro-Tips Hold your tooth pick at an ...bacteria over a second section of the plate, to create streak #2. Using a third sterile pipette tip, toothpick...bacteria over the last section of the plate, to create streak #3. Incubate plate with newly plated bacteria...

  3. Weighing Reagents Protocol

    Type
    Protocol
    ...using weighing paper, fold it in half first to create a crease and then place it unfolded onto the balance... sure you’re weighing all reagents precisely to create the solution you expect. Understanding how to obtain...

  4. Colony Formation Titering Assay

    Type
    Protocol
    ...likelihood of multiple integration events decreases as the dilution increases. Calculate the transduction units... remove cells and debris. Lentiviral titer can decrease during cycles of freeze-thaw. If you are freezing...

  5. TREAT-AD Plasmid Collection

    Type
    Collection
    ...validated resources for emerging targets in AD and creating high-quality, well-characterized lead molecules...

  8. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...adenosine deaminase to catalytically dead Cas13b creates a programmable RNA editor that converts adenosine...

  9. Depositor Collections

    Type
    Collection
    ...Rinehart FreeGenes Project Open Enzyme Collection Screening Multiplexed Overexpression of Regulatory Factors...

  10. MAPK Plasmids

    Type
    Collection
    ... plasmid collection for community. If you have created plasmids containing MAPK and would like to add ...

  12. Fluorescent Protein Guide

    Type
    Collection
    ...community-editable database for fluorescent proteins created by Talley Lambert. Nikon MicroscopyU (Link opens...

  20. Chemogenetics Plasmids

    Type
    Collection
    ...PSEMs). PSAMs are coupled to an ion pore domain to create a ligand-gated ion channel. Whether a PSAM is activating...
Showing: 201 - 220 of 960 results