We narrowed to 967 results for: Cre
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TypeCollection...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ...
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mTOR Pathway
TypeCollection... Tackling Cancers’ Drug Resistance with a New Screening Kit Background mTOR Pathway Plasmids mTOR Gene...mTORC1 promotes protein and lipid synthesis, and increases energy production through glycolysis to fuel these... Tackling Cancers’ Drug Resistance with a New Screening Kit (from David Sabatini & Kris Wood) References... -
Plant Plasmids and Resources
TypeCollection...Cloning The following collections are designed for creating plant expression vectors: A modular plasmid assembly... - Martin Parniske. GreenGate Cloning System to create plant expression vectors containing several cassettes... to understand how scientific publications are created. Papers from the plant biology literature are discussed... -
Protocol - How to Design Primers
TypeProtocol...also needs to avoid primer-primer annealing which creates primer dimers and disrupts the amplification process... -
Protocol - Over-Agar Antibiotic Plating
TypeProtocol... to do the spreading; the tip is gently bent to create an “L” shape, and then used like a cell spreader... -
Protocol - How to Purify DNA from an Agarose Gel
TypeProtocol...better resolution of bands? A couple simple ways to increase the resolution (crispness) of your DNA bands include... -
Protocol - How to Perform a Diagnostic Digest
TypeProtocol...with both and seeing both patterns you can be incredibly confident that you have the correct plasmid. ... -
Fluorescence Titering Assay
TypeProtocol... remove cells and debris. Lentiviral titer can decrease during cycles of freeze-thaw. If you are freezing... -
Molecular Biology Protocol - Restriction Digest of Plasmid DNA
TypeProtocol...(for instance, in a diagnostic digest), you can create a "Master Mix" consisting of all of the reaction... -
Protocol - How to Ligate Plasmid DNA
TypeProtocol...the reaction size as necessary - being sure to increase the amount of buffer proportionally. 1μL of ligase... -
Michael J Fox Foundation (MJFF) Plasmid Collection
TypeCollection...expressing genes relevant to Parkinson's disease created by the Michael J. Fox Foundation...topic. Michael J. Fox Foundation Plasmids MJFF has created a number of plasmids for expression of variants... -
CRISPR Plasmids - dCas9-FokI
TypeCollection...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs)....Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... -
KLF Research Plasmids
TypeCollection...plasmids and learn more about the scientists who have created and shared these plasmids....of this shared resource on Addgene. If you have created plasmids containing KLF, we encourage you to contribute... -
CRISPR Plasmids - Epigenetics
TypeCollection...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort... -
CRISPR Plasmids - Repress Gene Expression
TypeCollection...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort... -
CRISPR Plasmids - RNA Targeting
TypeCollection...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort... -
Structural Genomics Consortium Plasmids
TypeCollection...AmpR 26095 pFHMSP-LIC-N N/A Expression vector for secreted proteins with N-terminal hexahistidine tag and...AmpR 26100 pFHMSP-LIC-C N/A Expression vector for secreted proteins with C-terminal hexahistidine tag and... -
CRISPR Plasmids - Zebrafish
TypeCollection...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific... -
CRISPR Plasmids - Parasites
TypeCollection...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific... -
CRISPR Plasmids - CRISPR Transposases (CAST)
TypeCollection...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...Figure 1: Overview of the basic CAST mechanism. Created with BioRender.com. Browse, sort, or search the...